275,538 results
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Plasmid#12240DepositorPublicationInsertBmi1 (Bmi1 Mouse)
UseCre/Lox and LentiviralExpressionMammalianMutationCMV promoter upstream of mBmi-1. LoxP in 3' …Available sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLOX-CWBmi1
Plasmid#12240DepositorPublicationInsertBmi1 (Bmi1 Mouse)
UseCre/Lox and LentiviralExpressionMammalianMutationCMV promoter upstream of mBmi-1. LoxP in 3' …Available sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-NMHC II-C
Plasmid#10843DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNMHC II-C (Myh14 Mouse)
TagsEGFPExpressionMammalianAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-NMHC II-C
Plasmid#10843DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNMHC II-C (Myh14 Mouse)
TagsEGFPExpressionMammalianAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX_305_Ovalbumin
Plasmid#184924PurposeLentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.DepositorInsertOvalbumin (OVAL Chicken)
UseLentiviralExpressionMammalianMutationOur vector has an A188T mutation that does not im…PromoterPGKAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE (AAV8)
Viral prep#130995-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE (#130995). In addition to the viral particles, you will also receive purified pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE plasmid DNA. hSyn-driven, soma-targeted ChRmine-mScarlet expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmScarletAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV mGAD65(delE1) GFP WPRE SV40pA
Plasmid#177316PurposeTo produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.DepositorInsertmGAD65(delE1) promoter, GABAergic interneurons specific promoter
UseAAVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO mCherry (AAV Retrograde)
Viral prep#114471-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Flp-dependent)Available sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
MoClo CRISPR/Cas Toolkit for Plants
Plasmid kit#1000000159PurposePlasmids for gene editing using Cas nucleases and base editors in monocots and dicots.DepositorApplicationCloning and Synthetic Biology, Genome EditingVector typePlant ExpressionEditing typeCRISPRCloning typeGolden Gate (MoClo)Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP396
Plasmid#149336PurposeBacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobodyDepositorInsertanti-GFP nanobody
TagsBiotin acceptor peptide (Avi-tag), His14, and SUM…ExpressionBacterialAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 NSP16
Plasmid#141269PurposeGateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDONR223 SARS-CoV-2 NSP16
Plasmid#141269PurposeGateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Cbh_v5 AAV-CBE N-terminal
Plasmid#137175PurposeAAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE N-terminal; U6-protospacer
UseAAVMutationCas9 D10APromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cbh_v5 AAV-CBE N-terminal
Plasmid#137175PurposeAAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE N-terminal; U6-protospacer
UseAAVMutationCas9 D10APromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pREP-8xARE-GFP-SV40-BFP
Plasmid#134910PurposeNRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserts8xARE- minimal promoter - GFP
TagBFP
ExpressionMammalianPromoter8xARE - minimal promoter and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE
Plasmid#135391PurposeCre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.DepositorInsertTeLC-P2A-EYFP
UseAAVExpressionMammalianPromoterhSynapsinAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE
Plasmid#135391PurposeCre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.DepositorInsertTeLC-P2A-EYFP
UseAAVExpressionMammalianPromoterhSynapsinAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMAL-CellTag-V1 (Concentrated Lentiviral Prep)
Viral prep#115643-LVCPurposeReady-to-use Concentrated Lentiviral Prep particles produced from pSMAL-CellTag-V1 (#115643). In addition to the viral particles, you will also receive purified pSMAL-CellTag-V1 plasmid DNA. <p><p>Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.</p></p>DepositorPromoterMinimal CMVTagsEGFPAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-km
Plasmid#121999PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSGAb-km
Plasmid#121999PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HP17_KO7
Plasmid#120346PurposeHelper plasmid for production of phagemid ssDNA, based on M13KO7DepositorInsertCoding part of the M13KO7 helper phage genome
ExpressionBacterialPromoternative phage promotersAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HP17_KO7
Plasmid#120346PurposeHelper plasmid for production of phagemid ssDNA, based on M13KO7DepositorInsertCoding part of the M13KO7 helper phage genome
ExpressionBacterialPromoternative phage promotersAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-mCherry-(F30-2xPepper)10
Plasmid#129401PurposemCherry mRNA reporter tagged with (F30-2xPepper)10DepositorInsertmCherry-(F30-2xPepper)10
ExpressionMammalianPromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-mCherry-(F30-2xPepper)10
Plasmid#129401PurposemCherry mRNA reporter tagged with (F30-2xPepper)10DepositorInsertmCherry-(F30-2xPepper)10
ExpressionMammalianPromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
Tags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
Tags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-Halotag-FRB-EX-nes_pLX304
Plasmid#120913PurposeLentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosolDepositorInsertHA-Halotag-FRB-EX-NES
UseLentiviralExpressionMammalianAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-Halotag-FRB-EX-nes_pLX304
Plasmid#120913PurposeLentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosolDepositorInsertHA-Halotag-FRB-EX-NES
UseLentiviralExpressionMammalianAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP1039
Plasmid#119239Purposeexpresses Tn7 transposaseDepositorInserttnsABCD
ExpressionBacterialAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP1039
Plasmid#119239Purposeexpresses Tn7 transposaseDepositorInserttnsABCD
ExpressionBacterialAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmGFP-ADAR2
Plasmid#117929PurposeADAR2 expression plasmid.DepositorAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmGFP-ADAR2
Plasmid#117929PurposeADAR2 expression plasmid.DepositorAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-NLS-GFP
Plasmid#104061PurposeAAV expression of nuclear localized GFP with SV40 NLSDepositorHas serviceAAV PHP.eBInsertGFP
UseAAVPromoterCAGAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-NLS-GFP
Plasmid#104061PurposeAAV expression of nuclear localized GFP with SV40 NLSDepositorHas serviceAAV PHP.eBInsertGFP
UseAAVPromoterCAGAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)
Viral prep#100853-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases
Pooled library#101927PurposeBassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases. Coexpresses mCherry.DepositorExpressionMammalianUseCRISPRAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (AAV1)
Viral prep#100846-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (#100846). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV1)
Viral prep#26975-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
IGI-P0492 pHR-dCas9-NLS-VPR-mCherry
Plasmid#102245PurposeLentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.DepositorInsertdCas9-VPR-mCherry
UseCRISPR and LentiviralTagsNLS-VPR-mCherryExpressionMammalianPromoterCAGAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IGI-P0492 pHR-dCas9-NLS-VPR-mCherry
Plasmid#102245PurposeLentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.DepositorInsertdCas9-VPR-mCherry
UseCRISPR and LentiviralTagsNLS-VPR-mCherryExpressionMammalianPromoterCAGAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hLbCpf1(TYCV) (pY230)
Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hLbCpf1(TYCV) (pY230)
Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJYS3_ΔcrtYf
Plasmid#85542PurposeConstitutive transcription of FnCpf1 and crRNA of crtYfDepositorInsertsCpf1
crRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…ExpressionBacterialAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJYS3_ΔcrtYf
Plasmid#85542PurposeConstitutive transcription of FnCpf1 and crRNA of crtYfDepositorInsertsCpf1
crRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…ExpressionBacterialAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
Plasmid#63931PurposeEmpty AAV-RAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVPromoterpRAM and TREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
Plasmid#63931PurposeEmpty AAV-RAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVPromoterpRAM and TREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Talin-TS
Plasmid#83376PurposeFRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted between V447 an…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Talin-TS
Plasmid#83376PurposeFRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted between V447 an…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by