We narrowed to 32,487 results for: Eng;

1. pSABAD14-172

   Plasmid

   #61976

   Purpose

   PhoRadAC6 intein

   Depositor

   Hideo Iwai

   Article

   Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

   Insert

   PhoRadAC6 intein
   

   Tags

   GB1-H6

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSARSF53-165

   Plasmid

   #61817

   Purpose

   PhoRadAdC6 intein

   Depositor

   Hideo Iwai

   Article

   Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

   Insert

   PhoRadAdC6 intein
   

   Tags

   H6-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ134A

   Plasmid

   #69276

   Purpose

   Golden Gate entry vector; 4th gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRRL-MHCK7-R-GECO

   Plasmid

   #109374

   Purpose

   Expresses calcium indicator R-GECO in muscle cells

   Depositor

   Nenad Bursac

   Article

   Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

   Insert

   R-GECO
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS4083

   Plasmid

   #131739

   Purpose

   Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

   Depositor

   Christina Smolke

   Article

   Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

   Insert

   sTRSV
   

   Expression

   Mammalian

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPS-6803

   Plasmid

   #80458

   Purpose

   IPTG inducible expressoin of sucrose phosphate synthase derived from Synechocystis sp. PCC6803

   Depositor

   Daniel Ducat

   Article

   Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

   Insert

   Sucrose phosphate synthase
   

   Expression

   Bacterial

   Available Since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET24b-BR1.4

   Plasmid

   #199436

   Purpose

   expresses BAmRed1.4 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMRed1.4
   

   Tags

   his tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mEosEM-Lamin A

   Plasmid

   #132707

   Purpose

   application in Correlative light-electron microscopy

   Depositor

   Pingyong Xu

   Article

   Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   lamin A
   

   Tags

   mEosEM

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS4102

   Plasmid

   #131742

   Purpose

   Xanthine switch Xan-ACGAG

   Depositor

   Christina Smolke

   Article

   Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

   Insert

   Xanthine switch Xan-ACGAG
   

   Expression

   Mammalian

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLSLGFP

    Plasmid

    #51494

    Purpose

    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    GFP
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    NLS and flag

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPRyl_D17

    Plasmid

    #84611

    Purpose

    Introduces DSB at D17 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPRyl_A08

    Plasmid

    #84610

    Purpose

    Introduces DSB at A08 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET24b-BG1.3

    Plasmid

    #199437

    Purpose

    expresses BAmGreen1.3 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen1.3
    

    Tags

    his tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pECO200

    Plasmid

    #169765

    Purpose

    Express SpCas9 and Basta resistance gene

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Plant binary vector

    Expression

    Plant

    Promoter

    35S, AtU6

    Available Since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCVL SFFV d14GFP Donor

    Plasmid

    #31475

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14GFP
    

    Use

    Lentiviral

    Mutation

    Deleted 14 amino acids on the c terminus of eGFP

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPRyl_MFE1

    Plasmid

    #84612

    Purpose

    Introduces DSB at MFE1 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5F Smad3

    Plasmid

    #12625

    Depositor

    Rik Derynck

    Article

    Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-iRFP720

    Plasmid

    #89687

    Purpose

    pCAG promoter driving iRFP720

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iRFP720
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pECO300

    Plasmid

    #169766

    Purpose

    Express SpCas9 and Kanamycin resistance gene

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Plant binary vector

    Expression

    Plant

    Promoter

    35S, AtU6

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT_rHCaMP

    Plasmid

    #187107

    Purpose

    Expression of rHCaMP in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    rHCaMP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJPC12-ΔM13-mCherry-PR/PRM-GFP

    Plasmid

    #80859

    Purpose

    Reporter plasmid (mCherry and GFP)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    mCherry
    GFP
    

    Expression

    Bacterial

    Promoter

    Lambda Pr and Lambda Prm

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT7RNAP

    Plasmid

    #170101

    Purpose

    expresses T7 RNAP

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    T7-RNA polymerase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    p70a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHR_EF1a-->GssmCherry-PNE

    Plasmid

    #162157

    Purpose

    Lentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLNC Wnt-5bHA

    Plasmid

    #18034

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-5b (Wnt5b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pV1025

    Plasmid

    #111424

    Purpose

    Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAPInt-Dendra2-Nat

    Plasmid

    #236494

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    Dendra2
    

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJW1219

    Plasmid

    #61250

    Purpose

    CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6 promoter

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pST1374

    Plasmid

    #13426

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSETB-roGFP1-R12

    Plasmid

    #83311

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

    Insert

    roGFP1-R12
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

    Promoter

    T7

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pHAGE-TO-MCP-3XBFPnls

    Plasmid

    #75383

    Purpose

    MCP-3XBFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    3XBFP

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYPQ133D

    Plasmid

    #69289

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNJB91

    Plasmid

    #51519

    Purpose

    Gateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Type

    Empty backbone

    Use

    Gateway entry vector

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p3s-Cas9HC

    Plasmid

    #43945

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA epitope and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MSCV-FoxP3-R337Q/A372S-IRES-Thy1.1

    Plasmid

    #229619

    Purpose

    Mammalian expression of full length mouse FoxP3 (R337Q, A372S)

    Depositor

    Sun Hur

    Insert

    full length FoxP3, carrying double mutation R337Q/A372S (Foxp3 Mouse)
    

    Expression

    Mammalian

    Mutation

    R337Q/A372S

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p4xEGFP-N1

    Plasmid

    #122164

    Purpose

    Expression of 4xEGFP

    Depositor

    Georg Mayr

    Article

    Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

    Insert

    4xEGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p2lox-cAMPr R739A

    Plasmid

    #102618

    Purpose

    cAMPr with a point mutation R739A which theoretically increases its affinity for cAMP

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    R739A

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMEG_YLCas9_empty

    Plasmid

    #117826

    Purpose

    CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlpTEF_hCas9_YlCyc1TT
    

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-TRE3G-tdTomato

    Plasmid

    #194644

    Purpose

    Expresses tdTomato under the control of TRE3G

    Depositor

    Jun-Hyeong Cho

    Article

    Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

    Insert

    TRE3G-tdTomato
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJW1285

    Plasmid

    #61252

    Purpose

    CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E) targeting pha-1
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGP-FF

    Plasmid

    #13480

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Tags

    Gal11P

    Expression

    Bacterial

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAG504-frFAST

    Plasmid

    #160055

    Purpose

    Expresses frFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

    Insert

    frFAST
    

    Expression

    Mammalian

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGD425

    Plasmid

    #132381

    Purpose

    contains the sequence of mTurquoise2 for C-term fusions in the pGGD000 backbone (GreenGate)

    Depositor

    Rüdiger Simon

    Article

    Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

    Insert

    linker-mTurquoise2
    

    Use

    Greengate entry plasmid

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGEX Smad3

    Plasmid

    #12630

    Depositor

    Rik Derynck

    Article

    Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

44. pCA0.421

    Plasmid

    #203950

    Purpose

    Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pNBU2_erm_P1T_DP-GH023

    Plasmid

    #90317

    Purpose

    Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pIF4

    Plasmid

    #196069

    Purpose

    Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae

    Depositor

    Kabin Xie

    Article

    Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Promoter

    MoRP27 promoter

    Available Since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBTK1050

    Plasmid

    #209540

    Purpose

    BTK Type 1 homology arm for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    

    Use

    Synthetic Biology

    Available Since

    Feb. 9, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. pBTK1051

    Plasmid

    #209541

    Purpose

    BTK Type 5 homology arm for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    

    Use

    Synthetic Biology

    Available Since

    Feb. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. pADS

    Plasmid

    #19040

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    codon-optimized synthetic amorphadiene synthase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

50. BDS-2 3x WT (p53 binding site)

    Plasmid

    #16515

    Depositor

    Bert Vogelstein

    Article

    Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

    Insert

    p53 binding site (SFN Human)
    

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only