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163,387 results

Showing: 6801 - 6840 of 163387 results

  2. pCDF-Mm2

    Plasmid
    #197100
    Purpose
    To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati
    Depositor
    Kensaku Sakamoto
    Article
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    PylRS variant, Pyl tRNA, kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197100-plasmid-map-sequence-id-393189

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  9. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  12. mCherry-Vimentin-N-18

    Plasmid
    #55158
    Purpose
    Localization: Intermediate Filaments, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55158-plasmid-map-sequence-id-267740

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  14. pRSET.HaloTag.v7

    Plasmid
    #226197
    Purpose
    bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification
    Depositor
    HHMI-JRC Tool Translation Team, Tim Brown
    Insert
    HaloTag.v7
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    226197-plasmid-map-sequence-id-449504

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  18. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid
    #230996
    Purpose
    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.
    Depositor
    Michael Rape
    Article
    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:
    Type
    Empty backbone
    Use
    Tags
    AcGFP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    230996-plasmid-map-sequence-id-457945

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  33. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

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  35. pmal_c5x_RNAse_Inhib

    Plasmid
    #153314
    Purpose
    Bacterial expression of an RNase inhibitor
    Depositor
    Philippa Marrack, Drew Endy
    Insert
    RNAse Inhibitor
    Use
    Tags
    Maltose Binding Protein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153314-plasmid-map-sequence-id-298771

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  36. pCDH-EF1-IRES plum

    Plasmid
    #128072
    Purpose
    gfp was exchanged to plum
    Depositor
    Oskar Laur
    Insert
    plum
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    no
    Promoter
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128072-plasmid-map-sequence-id-255193

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  39. pET29a-human histone 4-in-One

    Plasmid
    #223237
    Purpose
    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4
    Depositor
    Weixing Zhao
    Inserts
    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    Use
    Tags
    Dual StrepII, His6, TEV protease site, and VSVG
    Expression
    Bacterial
    Mutation
    A103G
    Promoter
    T7
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223237-plasmid-map-sequence-id-443806

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Showing: 6801 - 6840 of 163387 results