Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 11,255 results for: NSI

Showing: 6921 - 6960 of 11255 results

  3. Scon_PheRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228372
    Purpose
    Expresses N-term transit peptide from Silene conica PheRS (Warren et al., 2023) with demonstrated plastid targeting; proof-of-concept for Plasmid 228371
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from Silene conica PheRS (Warren et al., 2023) with demonstrated plastid targeting.
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228372-plasmid-map-sequence-id-455843

    Enlarge

  4. Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228374
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228374-plasmid-map-sequence-id-455849

    Enlarge

  5. Shim_CytC_87.18_SHI04112_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228375
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Cytochrome C with demonstrated mito targeting; proof-of-concept for Plasmid 228371
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana Cytochrome C with demonstrated mito targeting.
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228375-plasmid-map-sequence-id-455854

    Enlarge

  6. Shim_GluRS_87.31.3_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228376
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Glutamate aminoacyl-tRNA synthetase evm.model.fragScaff_scaffold_87.31.3; determination of S. himalayana GluRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana GluRS evm.model.fragScaff_scaffold_87.31.3
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228376-plasmid-map-sequence-id-454891

    Enlarge

  7. Shim_MetRS_440.109_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228378
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Methionine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 440.10; determination of S. himalayana MetRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana MetRS evm.model.fragScaff scaffold 440.10
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228378-plasmid-map-sequence-id-455870

    Enlarge

  8. Shim_ThrRS_3895.3_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228380
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Threonine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3895.3; determination of S. himalayana ThrRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana ThrRS evm.model.fragScaff scaffold 3895.3
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228380-plasmid-map-sequence-id-455872

    Enlarge

  9. Shim_TrpRS_2406.7.1_SHI16564_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228381
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Tryptophan aminoacyl-tRNA synthetase (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA); to determine S. himalayana TrpRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana TrpRS (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228381-plasmid-map-sequence-id-455873

    Enlarge

  12. HIV-1-GFP ΔEnv CA E45A

    Plasmid
    #217438
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).
    Depositor
    Jarrod Johnson
    Article
    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    CA E45A
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    217438-plasmid-map-sequence-id-426692

    Enlarge

  13. HIV-1-GFP ΔEnv CA M66I

    Plasmid
    #217440
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).
    Depositor
    Jarrod Johnson
    Article
    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    CA M66I
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    217440-plasmid-map-sequence-id-426705

    Enlarge

  15. pSCrhaB2_ApyOAHIDS

    Plasmid
    #226246
    Purpose
    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS
    Depositor
    Wilfred van der Donk
    Article
    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103
    Inserts
    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    Use
    Tags
    Hexa-Histidine
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    226246-plasmid-map-sequence-id-450056

    Enlarge

  21. pIRGAL43C

    Plasmid
    #218278
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    GAL3 (S509P)
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218278-plasmid-map-sequence-id-433680

    Enlarge

  22. pIRGAL4A

    Plasmid
    #218277
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218277-plasmid-map-sequence-id-433678

    Enlarge

  23. pIRGAL3C

    Plasmid
    #218275
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    Use
    Tags
    Expression
    Yeast
    Mutation
    GAL3 (S509P)
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218275-plasmid-map-sequence-id-433661

    Enlarge

  26. pETDUET-GST AO7RE ∆238-245

    Plasmid
    #139318
    Purpose
    GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria
    Depositor
    Allan Weissman
    Article
    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3
    Insert
    AO7 aa 126-258 (RNF25 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    AO7 cloned into the first multiple cloning site o…
    Promoter
    T7 promoter-1
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    139318-plasmid-map-sequence-id-275576

    Enlarge

  37. mEos3.2trLAT-P

    Plasmid
    #203747
    Purpose
    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLAToC27A (Lat Mouse)
    Use
    Tags
    ER translocation signal - mEos3.2
    Expression
    Mammalian
    Mutation
    C27A
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203747-plasmid-map-sequence-id-403346

    Enlarge

  38. trLATm1PtoLmEos3.2

    Plasmid
    #203749
    Purpose
    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLATm1 (PtoL) (LAT Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    P8L, P17L
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203749-plasmid-map-sequence-id-403341

    Enlarge

  40. pLVX-mCherry-SPASTIN, L177D

    Plasmid
    #180649
    Purpose
    Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    SPASTIN
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Starts at M87, L177D mutation, has siRNA resistan…
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180649-plasmid-map-sequence-id-360019

    Enlarge

Showing: 6921 - 6960 of 11255 results