164,737 results

1. Myc-Foxo1 D256

   Plasmid

   #12145

   Depositor

   Domenico Accili

   Article

   Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

   Insert

   Foxo1 (Foxo1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a deletion truncating the protein after …

   Promoter

   CMV

   Available since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti_gRNA-Puro

   Plasmid

   #84752

   Purpose

   Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCW-HA-hRb-WT-puro

   Plasmid

   #212672

   Purpose

   Express tagged hRb

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194284

   Purpose

   EF1a-dCas9-KRAB-GFP with nontarget sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPD1101 Integration Test Circuit Vector

   Plasmid

   #139251

   Purpose

   Integration vector with TUs incorporated. Cells will be red and, if treated with doxycycline, blue.

   Depositor

   Joshua Leonard

   Insert

   Tus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28C-SARS-CoV-2-Nucleocapsid

   Plasmid

   #204402

   Purpose

   E. coli expression of full-length SARS-CoV-2 nucleocapsid

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAVS1-Puro CAG J591-scFV T-CAR

   Plasmid

   #171961

   Purpose

   Donor plasmid for J591 T-CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Harris et al J Immunol Regen Med. 2023 May;20:100074.

   Insert

   Synthetic J591-scFV CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 Connexin43-GFP-APEX2

   Plasmid

   #49385

   Purpose

   Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Connexin43 - EGFP - APEX2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCV020-tetQ

   Plasmid

   #232826

   Purpose

   Random transposon mutagenesis vector for Bacteroidales species - tetracycline

   Depositor

   Athanasios Typas

   Insert

   tetQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS mouse ER81 [TJ#197]

    Plasmid

    #16282

    Depositor

    Thomas Jessell

    Insert

    ER81 (Etv1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-CAG-GFP (AAV MaCPNS1)

    Viral Prep

    #37825-MaCPNS1

    Purpose

    Ready-to-use AAV MaCPNS1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the MaCPNS1 serotype, which permits efficient transduction of the PNS in rodents and the CNS and PNS (including ENS) in marmosets and rhesus macaques. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

12. GST-RH▲-MNase

    Plasmid

    #136292

    Purpose

    Expression of catalytic inactive RNase H fused to micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    RNase HI-D10R-E48R and MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBWB162

    Plasmid

    #140634

    Purpose

    pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

    Plasmid

    #173030

    Purpose

    To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    SARS-CoV-2 Nucleocapsid phosphoprotein
    

    Use

    Lentiviral

    Tags

    3xHA and IRES-mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Gag (truncated)

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

    Plasmid

    #29722

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-BE-zCas9

    Plasmid

    #101739

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9-UGI-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

    Plasmid

    #176285

    Purpose

    Cre-dependent AAV virus

    Depositor

    Shenqin Yao, The Allen Institute for Brain Science

    Article

    Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

    Insert

    TVA66T-dTomato-rabies Glycoprotein
    

    Use

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-Sec16A

    Plasmid

    #36155

    Purpose

    Expresses Sec16A as a GFP fusion

    Depositor

    David Stephens

    Article

    Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

    Insert

    Sec16A (SEC16A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

    Plasmid

    #110057

    Purpose

    Per2 transcription fluorescence reporter

    Depositor

    Eric Erquan Zhang

    Article

    Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    Per2 promoter and Venus (Per2 Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    NLS-D2

    Expression

    Mammalian

    Mutation

    Promoter

    Per2

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-Sac-Abeta(M1-40)

    Plasmid

    #71876

    Purpose

    Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-40) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

    Plasmid

    #124494

    Purpose

    Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Caspase-1 cleavage seq) T2A mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ER-mScarletI

    Plasmid

    #137805

    Purpose

    Visualization of the Endoplasmic Reticulum

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV1)

    Viral Prep

    #26975-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII

    Tags

    mCherry

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

24. AAV-LK03

    Plasmid

    #206512

    Purpose

    AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter

    Depositor

    Mark Kay

    Article

    Lisowski et al Nature. 2014 Feb 20;506(7488):382-6. doi

    Insert

    LK03 capsid
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    p5

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-HtrA2-FLAG

    Plasmid

    #15938

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CAG-GFP

    Plasmid

    #16664

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AiP13897 - pAAV-AiE0671m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3897)

    Plasmid

    #224038

    Purpose

    AiE0671m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CaMKIIa-EGFP

    Plasmid

    #50469

    Purpose

    EGFP under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

    Plasmid

    #106120

    Purpose

    Lentiviral expression of ALDH7A1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    ALDH7A1 (ALDH7A1 Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and Myc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Sumire-pRsetB

    Plasmid

    #193361

    Purpose

    GFP variant emit 414 nm fluorescence.

    Depositor

    Takeharu Nagai

    Article

    Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

    Insert

    Sumire
    

    Use

    E.coli expression

    Tags

    His X6 tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pF CAG luc-EGFP-cre puro

    Plasmid

    #67503

    Purpose

    Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    luciferase-EGFP-cre
    

    Use

    Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBabe puro HA PIK3CA H1047R

    Plasmid

    #12524

    Purpose

    Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1047R. Tumor-derived allele.

    Promoter

    Available since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a T7pCONS TIR-1 sfGFP

    Plasmid

    #154463

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-S5E2-ChR2-mCherry (AAV1)

    Viral Prep

    #135634-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Promoter

    Tags

    mCherry

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3-HA-ERK5

    Plasmid

    #65244

    Purpose

    Expression of HA tagged ERK5 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

    Insert

    ERK5 (MAPK7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A409T

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RSRF-Luc-2wt

    Plasmid

    #31818

    Depositor

    Astar Winoto

    Article

    Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

    Insert

    MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MEF2-dependent (-307 to -242 of Nur77/NR4A1)

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

38. pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215327

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    MUC5AC Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MUC5AC

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHAGE-TO-dCas9

    Plasmid

    #75381

    Purpose

    dCas9

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    Sp dCas9
    

    Use

    Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-RAB-EKARev

    Plasmid

    #118474

    Purpose

    ddRFP-based single-color biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. mCherry-Alpha-Actinin-19

    Plasmid

    #54975

    Purpose

    Localization: Alpha Actinin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    actinin alpha 1 (ACTN1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. HA-SFPQ

    Plasmid

    #166959

    Purpose

    Expresses HA-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3.1(+)-ExRai-AKAR2(T/A)

    Plasmid

    #161754

    Purpose

    Negative-control mutant for ExRai-AKAR2 biosensor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2(T/A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCDNA5-Flag-BRD4-BD

    Plasmid

    #90005

    Purpose

    expresses BD mutant BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed 140N to 140A, 433N to 433A

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAV.CAG.FLEX.iGluSnFR3.v857.GPI

    Plasmid

    #196218

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG.FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. mCherry-Rab5DN(S34N)

    Plasmid

    #35139

    Depositor

    Sergio Grinstein

    Article

    Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Dominant Negative- S34N

    Promoter

    CMV

    Available since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCW-HA-hRb-delta-CDK-puro

    Plasmid

    #212673

    Purpose

    Express tagged hRb delta-CDK phosphosite mutant

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    Rb (RB1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    All 15 CDK phospho-sites are mutated to alanines

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGL4 mIL-17 2kb promoter

    Plasmid

    #20124

    Depositor

    Warren Strober

    Article

    Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

    Insert

    mIL-17 (Il17a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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49. mTagGFP2-Clathrin-15

    Plasmid

    #56413

    Purpose

    Localization: Clathrin Vesicles, Excitation: 483, Emission: 506

    Depositor

    Michael Davidson

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    mTagGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

50. CROPSeq-BFP

    Plasmid

    #196718

    Purpose

    CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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