167,825 results

1. pU6-pegRNA-GG-acceptor

   Plasmid

   #132777

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   Exchangeable cassette
   

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-mCherry-Nanobody

   Plasmid

   #70696

   Purpose

   Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   mCherry-Nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   March 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTM_YR375_SUMO_Protease_001

   Plasmid

   #190063

   Purpose

   Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   ULP1_YEAST (ULP1 Budding Yeast)
   

   Tags

   8X His-MBP (Maltose Binding Protein)

   Expression

   Bacterial

   Mutation

   Construct contains residues 347 – 621

   Promoter

   T7 lac

   Available Since

   Jan. 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAAV-syn-dLight1.3b (AAV9)

   Viral Prep

   #135762-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-syn-dLight1.3b (#135762). In addition to the viral particles, you will also receive purified pAAV-syn-dLight1.3b plasmid DNA. Syn-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Promoter

   Syn

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

5. Cytoplasmic PancACe

   Plasmid

   #215708

   Purpose

   Expresses acetyl-CoA biosensor in the cytoplasm

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and cytoplasmic localization sequence

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-2xFYVE

   Plasmid

   #140047

   Purpose

   Expresses GFP-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available Since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-hM3D(Gq)-mCherry (AAV8)

   Viral Prep

   #50474-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGP-AAV-syn-iGABASnFR2-WPRE (AAV5)

   Viral Prep

   #218874-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-syn-iGABASnFR2-WPRE (#218874). In addition to the viral particles, you will also receive purified pGP-AAV-syn-iGABASnFR2-WPRE plasmid DNA. Syn-driven expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Promoter

   Syn

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

9. pCRIS-PITChv2-dTAG-Puro (BRD4)

   Plasmid

   #91796

   Purpose

   PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   FKBP_F36V-2xHA-P2A-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7II-2N4Rtau

    Plasmid

    #177653

    Purpose

    Recombinant protein synthesis of human Tau

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    Tau (2N4R) (MAPT Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

    Plasmid

    #110854

    Purpose

    Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    sgRNA scaffold with spacer
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    U6

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209722

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #49425

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

    Depositor

    Daniel Klionsky

    Article

    Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Promoter

    ATG8

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-TO-NFIA.1-SOX9

    Plasmid

    #182310

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-CMV-jGCaMP8f

    Plasmid

    #162373

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MoClo Yeast Toolkit

    Plasmid Kit

    #1000000061

    Purpose

    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes

    Depositor

    John Dueber

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

20. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

    Plasmid

    #168291

    Purpose

    optimized miniCMV (OminiCMV)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

    Plasmid

    #168305

    Purpose

    mediated knock-in of sgRNA precursor

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    SaKKH-EGFP-U6-sgRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1alpha, CBh

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid

    #183532

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Mutation

    None

    Promoter

    nEF

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV R-CEPIA1er

    Plasmid

    #58216

    Purpose

    Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    R-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV.CAG.(mito).cpSFGFP.HaloTag

    Plasmid

    #214925

    Purpose

    Expresses mitochondrially targeted non-responsive control

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).cpSFGFP.HaloTag
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-FLEX-EGFPL10a (AAV5)

    Viral Prep

    #98747-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Nathaniel Heintz, Alexander Nectow, Eric Schmidt

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Promoter

    EF1a

    Tags

    EGFP (Cre-dependent)

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

26. sortase A pentamutant (eSrtA) in pET29

    Plasmid

    #75144

    Purpose

    evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

    Depositor

    David Liu

    Article

    Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

    Insert

    sortase A pentamutant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T. Deleted amino a…

    Promoter

    T7

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCMV-PE6a

    Plasmid

    #207851

    Purpose

    Mammalian expression of PE6a prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6a
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. tet-pLKO-sgRNA-puro

    Plasmid

    #104321

    Purpose

    3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

    Depositor

    Nathanael Gray

    Article

    Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    H1/TO

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. tau/pET29b

    Plasmid

    #16316

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    tau (MAPT Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMitoTimer

    Plasmid

    #52659

    Purpose

    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

    Depositor

    Zhen Yan

    Article

    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

    Insert

    DsRed1-E5
    

    Use

    Dsred

    Tags

    human cytochrome c oxidase subunit VIII mitochond…

    Promoter

    CMV

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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31. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. CD63-pEGFP C2

    Plasmid

    #62964

    Purpose

    full length human CD63 cloned into pEGFP C2

    Depositor

    Paul Luzio

    Insert

    CD63 molecule (CD63 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CD63 has 1 silent mutation compared to the coding…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHIV-Luc-ZsGreen

    Plasmid

    #39196

    Depositor

    Bryan Welm

    Inserts

    Firefly Luciferase Luc2P
    ZsGreen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-alpha and EF1-alpha

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-myc-CUL3

    Plasmid

    #19893

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 3 (CUL3 Human)
    

    Tags

    3xmyc

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCCL-CellCycle

    Plasmid

    #132429

    Purpose

    Expressing Fucci cell cycle indicator in mammalian cells

    Depositor

    John M. Greally

    Article

    Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

    Insert

    hCdt, hGeminin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUC19_HDRT_TRAC_CD19-CAR

    Plasmid

    #183473

    Purpose

    Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

    Inserts

    TRAC (TRAC Human)
    CD19-specific CAR transgene insert
    TRAC (TRAC Human)
    

    Use

    Unspecified

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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37. Dual-sgRNA_hU6-mU6

    Plasmid

    #154194

    Purpose

    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Rtn3L-mNeonGreen

    Plasmid

    #169034

    Purpose

    Express human Rtn3L in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn3L (RTN3 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-CAG-GFP (AAV Retrograde trial size)

    Viral Prep

    #37825-AAVrg.T

    Purpose

    Ready-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

40. lenti dCAS-VP64_Blast (Lentiviral Prep)

    Viral Prep

    #61425-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from lenti dCAS-VP64_Blast (#61425). In addition to the viral particles, you will also receive purified lenti dCAS-VP64_Blast plasmid DNA.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Promoter

    EF1a

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only