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Plasmid#1654DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRNAiTagsT7p, T7p, lacZN, OriF1>>, OriF1<<ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only
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pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE
Plasmid#60906PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Dual-sgRNA_hU6-mU6
Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP(1-10)
Plasmid#70219PurposeExpresses GFP(1-10) in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertGFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tdTomato
Plasmid#83029Purposeexpresses tdTomato fluorescent protein under the CAG promoterDepositorInserttdTomato
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Glycogene CRISPR Library
Pooled Library#140961PurposeTargets human genes involved in cellular glycosylation.DepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Nucleolin
Plasmid#28176DepositorInsertNucleolin (NCL Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-CMV-bArrestin2-TEV
Plasmid#107245PurposeVector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.DepositorInsertARRB2
UseTagsTEV(NIA), tobacco etch virus NIAExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-GW
Plasmid#60323PurposeThis is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).DepositorTypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV8)
Viral Prep#154868-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-10His-mStayGold (E138D)
Plasmid#211363PurposeMammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
UseTags10xHis-tagExpressionMammalianMutationE138DPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1 and PGKAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF)
Pooled Library#1000000111PurposeGenome-wide CRISPR gRNA pooled library for activation of human genesDepositorAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (AAV8)
Viral Prep#50454-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (#50454). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine plasmid DNA. Synapsin-driven, Cre-dependent, excitatory hM3D DREADD expression, with physically separate mCitrine expression for cell body labeling. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCitrine (Cre-dependent)Available sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2
Plasmid#132775PurposePrime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3bDepositorInsertPE2
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagExpressionMutationPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCAS9-VP64_GFP
Plasmid#61422PurposeExpresses dCAS9-VP64 activator with 2A GFPDepositorHas ServiceConcentrated Lentiviral PrepInsertdCAS9(D10A,H840A)-VP64_2A_GFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PPRE X3-TK-luc
Plasmid#1015PurposePPAR reporter. 3xDR1 sites upstream of a luciferase reporter.DepositorInsertPPRE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8m-WPRE (AAV9)
Viral Prep#176751-AAV9PurposeReady-to-use AAV9 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaTagsNoneAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (AAV8)
Viral Prep#130909-AAV8PurposeReady-to-use AAV8 particles produced from AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (#130909). In addition to the viral particles, you will also receive purified AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. CAG-driven, Flp-dependent expression of ChrimsonR-tdTomato. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Flp-dependent)Available sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-iGluSnFR4s-NGR-WPRE
Plasmid#234437PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4s-NGR
UseAAVTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
FRESCA-syntide
Plasmid#132415PurposeFRET-based sensor for CaMKII activityDepositorInsertFRESCA-syntide
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag
Plasmid#208051PurposeEmpty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with FlagDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL2-p21 promoter-Luc
Plasmid#33021DepositorInsertp21 promoter (CDKN1A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterp21Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tdTomato (codon diversified)
Plasmid#59462PurposeAAV-mediated expression of tdTomato (codon diversified) under the CAG promoterDepositorHas ServiceAAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserttdTomato
UseAAVTagsExpressionMammalianMutationcodon diversifiedPromoterCAGAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF2545
Plasmid#144038PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCEBPB (CEBPB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCS2
Plasmid#46954PurposeCloning plasmid for the generation of Knock-In in human cells using rAAV.DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTn Donor
Plasmid#236211PurposeArabinose-inducible Tn5 InducTn-seq plasmidDepositorInsertNone
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcBac2.tR4-OMeYRS/GFP*
Plasmid#50831Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Tyr tRNA cassette
EGFP*
OMeYRS
two copy Tyr tRNA cassette
UseBaculoviralTagsHis, Myc-6xHis, and WPREExpressionInsectMutationY39TAG and able to charge various unnatural amino…PromoterCAG, CMV, and U6 and H1Available sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-puro
Plasmid#125839Purposemodifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.PI.EGFP.WPRE.bGH (AAV9)
Viral Prep#105530-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsEGFPAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-taCasp3-TEVp (AAV5)
Viral Prep#45580-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW1169
Plasmid#236583PurposeTRV1DepositorInsertTRV1
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Basic Vector
Plasmid#212936PurposeFirefly luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
IFN-Beta_pGL3
Plasmid#102597PurposeIFN-beta promoter driving luciferase. Use in IFN reporter assay.DepositorInsertHuman IFN-Beta promoter (IFNB1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOs
Plasmid#189931PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only