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165,839 results

Showing: 851 - 900 of 165839 results

  2. pAAV-syn-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234435
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234435-plasmid-map-sequence-id-465726

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  4. pMX-Flag-GFP_HOMER2

    Plasmid
    #227953
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    HOMER2 (HOMER2 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227953-plasmid-map-sequence-id-455411

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  5. pET28a SnoopTag-MBP

    Plasmid
    #72323
    Purpose
    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-MBP
    Use
    Tags
    N-terminal His6 tag. MBP is fused at the C-termin…
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72323-plasmid-map-sequence-id-137256

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  6. mCherry-VASP-5

    Plasmid
    #55151
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    VASP
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A209T in VASP
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55151-plasmid-map-sequence-id-424687

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  11. pCAGGS-FuG-E (P440E)

    Plasmid
    #119978
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E (P440E) envelope coating virus particle with NeuRet.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci Methods. 2018 Oct 19;311:147-
    Insert
    Fusion Glycoprotein type E (P440E)
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Expression
    Mutation
    Promoter
    CAGGS
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119978-plasmid-map-sequence-id-232261

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  24. pHLmMBP-1

    Plasmid
    #72343
    Purpose
    Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72343-plasmid-map-sequence-id-132066

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  27. pAAV-hSyn-EGFP (AAV Retrograde)

    Viral Prep
    #50465-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. Synapsin-driven EGFP-expression control. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only

  28. pN094

    Plasmid
    #167155
    Purpose
    MoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-npgA-ocsT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167155-plasmid-map-sequence-id-335276

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  29. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  31. Ubi:eGFP-ccdb + RUBY

    Plasmid
    #233309
    Purpose
    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    Ubi:eGFP-ccdb + RUBY
    Use
    Gateway destination
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Available Since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233309-plasmid-map-sequence-id-464380

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  35. pKAR5

    Plasmid
    #149461
    Purpose
    Broad-Host range expression vector, RK2 and pUC origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.
    Depositor
    Christopher Reisch
    Article
    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629
    Insert
    AraC-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    149461-plasmid-map-sequence-id-311913

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  37. TFORF2950

    Plasmid
    #144426
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MAFB (MAFB Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144426-plasmid-map-sequence-id-407318

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  38. pCS2+-HypocratesCS

    Plasmid
    #161960
    Purpose
    Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives
    Depositor
    Dmitry Bilan
    Article
    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:
    Insert
    Hypocrates
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Cysteine 355 to Serine
    Promoter
    CMV, SP6
    Available Since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161960-plasmid-map-sequence-id-324303

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  39. pIC247

    Plasmid
    #69741
    Purpose
    Retroviral expression of Borealin
    Depositor
    Iain Cheeseman
    Insert
    Borealin (CDCA8 Human)
    Use
    Retroviral
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  42. pLenti-CMV-PtenC124S-Puro

    Plasmid
    #135677
    Purpose
    Constitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    C124S
    Promoter
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135677-plasmid-map-sequence-id-327588

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  46. P445-FBP_17

    Plasmid
    #139708
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available Since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139708-plasmid-map-sequence-id-296601

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Showing: 851 - 900 of 165839 results