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171,694 results

Showing: 851 - 900 of 171694 results

  2. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  5. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

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  6. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid
    #216152
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216152-plasmid-map-sequence-id-437762

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  12. pGP-AAV-syn-jGCaMP8m-WPRE (AAV PHP.eB)

    Viral Prep
    #162375-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  13. PB CAG-tdTomato

    Plasmid
    #133569
    Purpose
    CAG tdTomato piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133569-plasmid-map-sequence-id-274028

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  14. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Available Since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122650-plasmid-map-sequence-id-437776

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  16. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  18. pUC57-LbNOX

    Plasmid
    #75285
    Purpose
    This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Insert
    LbNOX
    Tags
    C-terminal FLAG tag with linker
    Mutation
    Human codon optimized
    Available Since
    May 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    75285-plasmid-map-sequence-id-258257

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  19. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid
    #111731
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q145 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    111731-plasmid-map-sequence-id-226895

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  20. pAAV-Ef1a-DIO mScarlet (AAV5)

    Viral Prep
    #131002-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO mScarlet (#131002). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO mScarlet plasmid DNA. EF1a-driven, Cre-dependent mScarlet expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie
    Promoter
    EF1a
    Tags
    mScarlet (Cre-dependent)
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  21. Human SAM library in lentiSAMv2 backbone

    Pooled Library
    #1000000078
    Purpose
    Genome-wide CRISPR gRNA pooled library for activation of human genes
    Depositor
    Feng Zhang
    Available Since
    June 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. TFORF2545

    Plasmid
    #144038
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CEBPB (CEBPB Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144038-plasmid-map-sequence-id-392329

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  29. pAAV-CAG-dLight1.3b (AAV9)

    Viral Prep
    #125560-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci
    Promoter
    CAG
    Available Since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  34. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV9)

    Viral Prep
    #105540-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Synapsin-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    March 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  35. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    Cloning and assembly of DNA constructs for chromosomal integration in E. coli.
    Depositor
    Drew Endy
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Clonetegration
    Available Since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. CRISPi Kit

    Plasmid Kit
    #1000000136
    Purpose
    Plasmids for performing CRISPR/Cas9 mediated gene and genome editing in Pichia pastoris.
    Depositor
    Brigitte Gasser
    Application
    Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  38. C321.ΔA.exp

    Bacterial Strain
    #49018
    Purpose
    This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C
    Depositor
    George Church
    Article
    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d
    Bacterial Resistance
    Bleocin (Zeocin)
    Species
    Escherichia coli
    Available Since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  47. pLKO.1 puro

    Plasmid
    #8453
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    July 18, 2005
    Availability
    Academic Institutions and Nonprofits only
    8453-plasmid-map-sequence-id-237918

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Showing: 851 - 900 of 171694 results