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163,819 results

Showing: 851 - 900 of 163819 results

  9. SunTagng22aa

    Plasmid
    #106438
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106438-plasmid-map-sequence-id-309504

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  15. eGFP-ova-C1

    Plasmid
    #163524
    Purpose
    Expresses both eGFP and chicken ovalbumin in mammalian cells
    Depositor
    Thomas Weber
    Insert
    enhanced-GFP-chicken ovalbumin tandem
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163524-plasmid-map-sequence-id-321577

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  16. IDG_TMEM63B_OE_1

    Plasmid
    #161682
    Purpose
    Overexpresses TMEM63B
    Depositor
    Mike McManus
    Insert
    TMEM63B (TMEM63B Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    161682-plasmid-map-sequence-id-319709

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  28. THBS1-bio-His

    Plasmid
    #53417
    Purpose
    Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    THBS1 (THBS1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53417-plasmid-map-sequence-id-83207

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  30. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Article
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100547-plasmid-map-sequence-id-313417

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  32. AcGFP1-N1

    Plasmid
    #54705
    Purpose
    Localization: N1 Cloning Vector, Excitation: 475, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    AcGFP1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    54705-plasmid-map-sequence-id-162699

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  33. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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  34. TFORF2004

    Plasmid
    #143330
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXD1 (FOXD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143330-plasmid-map-sequence-id-400291

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  39. AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) (AAV PHP.eB)

    Viral Prep
    #191726-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) (#191726). In addition to the viral particles, you will also receive purified AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) plasmid DNA. Expression of SYFP2 in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Promoter
    Tags
    None
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only

  43. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV Retrograde)

    Viral Prep
    #44361-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only

  47. pET21a IHF

    Plasmid
    #26641
    Depositor
    Phoebe Rice
    Insert
    IHF
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 22, 2010
    Availability
    Academic Institutions and Nonprofits only

  48. pHN922

    Plasmid
    #178975
    Purpose
    Expression of GST-His8-GFP-nanobody
    Depositor
    Kazuhiro Abe
    Insert
    GST-His8-GFP-nanobody
    Use
    Affinity Reagent/ Antibody
    Tags
    GST, His8
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178975-plasmid-map-sequence-id-354968

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  49. p-dCas9-HDAC1-H141A-Hygro

    Plasmid
    #104417
    Purpose
    transient expression of dCas9-dHDAC1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dHDAC1 (HDAC1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H141A
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104417-plasmid-map-sequence-id-204223

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Showing: 851 - 900 of 163819 results