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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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167,795 results

Showing: 7241 - 7280 of 167795 results

  7. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133451-plasmid-map-sequence-id-267048

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  13. pAAV-hSyn-Cre-P2A-dTomato (AAV2)

    Viral Prep
    #107738-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Rylan Larsen
    Promoter
    Syn
    Tags
    dTomato (physically separate, not a fusion protein)
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. pCMV-Nopt

    Plasmid
    #231704
    Purpose
    Mammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.
    Depositor
    Ron Geller
    Article
    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1
    Insert
    VSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
    Use
    Vsv expression
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231704-plasmid-map-sequence-id-459826

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  22. CDH5-AbR-GFP

    Plasmid
    #122970
    Purpose
    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.
    Depositor
    Budd A. Tucker
    Inserts
    CDH5p
    CopGFP
    Zeocin
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    NA
    Available Since
    May 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122970-plasmid-map-sequence-id-244602

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  24. WT FL USP7

    Plasmid
    #227214
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227214-plasmid-map-sequence-id-452331

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  31. Tn7 integration plasmid

    Plasmid
    #154134
    Purpose
    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.
    Depositor
    Shimon Bershtein
    Article
    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038
    Inserts
    left and right end of Tn7 arm
    Spectinomycin resistant gene
    Expression
    Bacterial
    Promoter
    Spectinomycin resistance gene promoter and no
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154134-plasmid-map-sequence-id-306811

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  34. pLEXSY-hyg2.1_LtIFT43_Flag

    Plasmid
    #194433
    Purpose
    Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae
    Depositor
    Alan Brown
    Article
    Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.
    Insert
    Intraflagellar transport complex subunit 43
    Use
    Leishmania tarentolae expression
    Tags
    3X Flag and Linker
    Promoter
    L.donovani promoter elements
    Available Since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    194433-plasmid-map-sequence-id-383732

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  38. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113035-plasmid-map-sequence-id-224728

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  39. pAS134

    Plasmid
    #211505
    Purpose
    AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    Venus
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211505-plasmid-map-sequence-id-417792

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Showing: 7241 - 7280 of 167795 results