169,450 results
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pSRK2706
Plasmid#78571PurposeExpresses untagged human rhinovirus 3C protease in E. coliDepositorAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
TagsmCherryExpressionBacterial and MammalianPromoterCMVAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-pBAD
Plasmid#54531PurposeLocalization: Protein Expression Vector , Excitation: 549, Emission: 565DepositorTypeEmpty backboneTags6xHis and mOrange2ExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast
Plasmid#205555PurposeExpress EPN24-MCP-IRES-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC019 - LwCas13a from Leptotrichia wadei
Plasmid#91909PurposeExpresses LwCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLwCas13a and guide expression cassette
UseCRISPRExpressionBacterialAvailable SinceOct. 4, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
Plasmid#65418PurposeAAV packaging vector for CamKIIa driven KOR DREADD expressionDepositorAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-MAFB-IRF8-CEBPA
Plasmid#190720PurposeFor microglia differentiation from iPSCDepositorInsertMAFB-IRF8-CEBPA
ExpressionMammalianAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-STING-sh5
Plasmid#127647PurposeKnock-down of human STINGDepositorInsertSTING shRNA (STING1 Human)
UseLentiviralAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP
Plasmid#188902PurposedCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertdCas9-KRAB(Kox1)-MeCP2
UseLentiviralTagsNLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…PromoterSFFVAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab4a-7
Plasmid#55125PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
6xAP-1_RE::Renilla
Plasmid#178317PurposeAP-1 Renilla luciferase reporterDepositorInsertAP-1 Renilla Reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
spCas9-BlastR (pCBhCas9-BlastR)
Plasmid#71489PurposeExpresses SpCas9 and Blasticidin resistanceDepositorInsertspCas9
PromoterCBhAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV mClover3-Galectin-3
Plasmid#215376PurposeExpression of the endolysosomal damage marker Galectin-3 with an N-terminal mClover3 tag.DepositorInsertLGALS3 (LGALS3 Human)
ExpressionMammalianAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP12316 - pAAV-AiE0467m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2316)
Plasmid#230109PurposeAiE0467m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2
UseAAVPromoterminBGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-minCMV-mCherry
Plasmid#27970DepositorInsertmCherry
UseAAVExpressionMammalianAvailable SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-JAK2
Plasmid#23915DepositorInsertJAK2 (JAK2 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLBS-mScarlet
Plasmid#129337Purposecytosol marker, lentivirusDepositorInsertmScarlet (Addgene 85042)
UseLentiviralAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Ef1a-CreN-InteinN
Plasmid#187614PurposeAAV vector that encodes N-terminal portion of split-Intein-mediated split-CreDepositorInsertCreN-InteinN
UseAAVExpressionMammalianAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 TEV (full-length)
Plasmid#64276PurposeExpresses tobacco etch virus protease (TEV)DepositorInsertTobacco etch virus (TEV) protease
ExpressionMammalianPromoterCMVAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
E. coli BW25113 ΔtnaA ΔtrpR
Bacterial Strain#205015PurposeUsed for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)DepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable SinceAug. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
mApple-Alpha-V-Integrin-25
Plasmid#54866PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592DepositorAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-Zim3-MeCP2
Plasmid#220143Purposedoxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repressionDepositorInsertdCas9-Zim3-MeCP2
UseCRISPR and Synthetic Biology; PiggybacExpressionMammalianPromoterTre-3GAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-EGFP (AAV1)
Viral Prep#59331-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-FLEX-EGFP (#59331). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-EGFP plasmid DNA. CAG-driven, Cre-dependent expression of EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFPAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastbac1 Nterminal GST
Plasmid#128058PurposeBaculovirus expression vector with N terminal GSTDepositorTypeEmpty backboneTagsGSTExpressionInsectAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse Brie kinome pooled library, guides 1-4 in lentiGuide-Puro backbone
Pooled Library#75316PurposeMouse sgRNA library in backbone lentiGuide-Puro targeting 713 kinase genes and containing 2,852 unique sgRNAs along with 100 non-targeting controlsDepositorAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYS29
Plasmid#202086PurposeParent vector for producing universal minicircle donors for HaloTag N-terminal knock-inDepositorInsertNT HygR-T2A-Halo Minicircle Parent
UseCRISPRTagsHaloTagPromoterNoneAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-Tn5
Plasmid#184286PurposeAnti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmKappa-Tn5
Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.3G_X7
Plasmid#171213Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sitesDepositorInsertN/A; empty backbone
UseLentiviral and RNAiExpressionMammalianAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-BRD9
Plasmid#65380PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60229PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianPromoterCBh and U6Available SinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHEE401E
Plasmid#71287PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 enhancer fused to EC1.1 promoter and U6-26p…Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR
Plasmid#82024PurposeLentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selectionDepositorInsertmKate2-2xNLS
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC007 - pRFP constitutive expression
Plasmid#79156PurposeRFP constitutively expressedDepositorInsertRFP
Available SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro
Plasmid#227256PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR3__TACC3
Plasmid#116818PurposeLentiviral expression of FGFR3__TACC3 fusionDepositorInsertFGFR3__TACC3
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only
