We narrowed to 14,002 results for: flag

1. LentiV_Puro

   Plasmid

   #111886

   Purpose

   Lentiviral expression of cDNA with puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-Tag1-NS3

   Plasmid

   #17645

   Depositor

   Xin Wang

   Article

   Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

   Insert

   HCV NS3 (POLY Hepatitis C virus)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GreenT-EC - Display - CyRFP1

   Plasmid

   #193943

   Purpose

   Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC
   

   Use

   Tags

   Igk_secretion - HA-Flag and PDGFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRG/III-hs-MBP-CB2-HF

   Plasmid

   #121682

   Purpose

   Bacterial expression of cannabinoid receptor CB2 fusion protein

   Depositor

   Richard Henderson, Reinhard Grisshammer

   Article

   Calandra et al Biotechnology Letters

   Insert

   peripheral cannabinoid receptor (CNR2 Human)
   

   Use

   Tags

   His6 Flag tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDH-UTY-F

   Plasmid

   #40617

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   UTY (UTY Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   EF1

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCS2-UTY-F

   Plasmid

   #17439

   Purpose

   Expression of UTY in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   UTY (UTY Human)
   

   Use

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SadCas9 VPR

   Plasmid

   #188514

   Purpose

   Expresses FLAG tagged SadCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-FNLSNG-P2A-Puro

   Plasmid

   #136900

   Purpose

   Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-OSF-PP

   Plasmid

   #154247

   Purpose

   backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Type

   Empty backbone

   Use

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pShuttle-FEN1hWT

    Plasmid

    #35027

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1 (FEN1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

    Plasmid

    #165429

    Purpose

    Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    FHS-paCaMKII
    

    Use

    AAV

    Tags

    FHS(Flag, HisX6, Strep) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII0.4

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCW57-mcGAS-LL/RK-NLS/D307A

    Plasmid

    #185459

    Purpose

    Inducible nuclear cGAS with D307A mutation

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K, D307A

    Promoter

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IRES-GFP-AXL-KD

    Plasmid

    #65498

    Purpose

    Expresses kinase dead AXL in mammalian cells with cistronic EGFP

    Depositor

    Aaron Meyer

    Article

    Meyer et al Cell Syst. 2015 Jul 29;1(1):25-36.

    Insert

    Tyrosine-protein kinase receptor UFO (AXL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K567R

    Promoter

    CMV

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEntr-GATA4flbio

    Plasmid

    #32937

    Depositor

    William Pu

    Article

    He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

    Insert

    GATA4 (Gata4 Mouse)
    

    Use

    Gateway

    Tags

    flag-bio

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-CyclinF

    Plasmid

    #32975

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

    Insert

    CYCLIN F (CCNF Human)
    

    Use

    Tags

    Flag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

16. TAL2451

    Plasmid

    #36840

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HMGA2-TALEN_Right (Hmga2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pET28 Set9 wt

    Plasmid

    #24082

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

18. BII-ChBtW-iresFUCCI

    Plasmid

    #133371

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA Set9 mutant

    Plasmid

    #24085

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H297A

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GreenT-EC.b - Display - CyRFP1

    Plasmid

    #193944

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.b
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GreenT-EC.c - Display - CyRFP1

    Plasmid

    #193945

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.c
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Use

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFsynW SYB2 reporter

    Plasmid

    #197276

    Purpose

    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQAT03

    Plasmid

    #223375

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AsdCas12a KRAB

    Plasmid

    #188502

    Purpose

    Expresses FLAG tagged AsdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. R4227X-F

    Plasmid

    #21371

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG (following D4226)

    Expression

    Mammalian

    Mutation

    Changed R4227 to stop codon

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

29. LbdCas12a VPR

    Plasmid

    #188513

    Purpose

    Expresses FLAG tagged LbdCas12a VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX 4T1 hBad

    Plasmid

    #35567

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    bad (BAD Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pMCS-rybozyme-IRES-CAS9

    Plasmid

    #64668

    Purpose

    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

    Depositor

    Wataru Fujii

    Article

    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

    Inserts

    CAS9
    HDV ribozyme
    

    Use

    CRISPR

    Tags

    Flag, Internal ribosome entry site, and guide RNA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SETMAR-Cas9

    Plasmid

    #186701

    Purpose

    Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETMAR-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SETD2(SET)-Cas9

    Plasmid

    #186700

    Purpose

    Plasmid encoding SETD2-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETD2(SET)-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCS2 hSmad1-WT

    Plasmid

    #22989

    Depositor

    Edward De Robertis

    Article

    Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

    Insert

    Smad1 (SMAD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA3-R4-uAb

    Plasmid

    #101800

    Purpose

    Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

    Depositor

    Matthew DeLisa

    Article

    Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

    Insert

    R4-uAb
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pN1-Lck-GCaMP3

    Plasmid

    #26974

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

    Insert

    Lck-GCaMP3
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti-FNLSHiFiNG-P2A-Puro

    Plasmid

    #136903

    Purpose

    Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFiNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAd-Klf4

    Plasmid

    #19770

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Science. 2008 Nov 7;322(5903):945-9. doi

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LbdCas12a KRAB

    Plasmid

    #188503

    Purpose

    Expresses FLAG tagged LbdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCF CREB M1

    Plasmid

    #22969

    Depositor

    Marc Montminy

    Article

    Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

    Insert

    CREB (Creb1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pN1-Lck-GCaMP2

    Plasmid

    #24794

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

    Insert

    Lck-GCaMP2
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. P823_eSpCas9(1.1)-RecA

    Plasmid

    #87264

    Purpose

    Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pFLEX+mCyclin D1

    Plasmid

    #86793

    Purpose

    mammalian expression of cyclin D1

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Matsushime et al Cell. 1991 May 17;65(4):701-13.

    Insert

    cyclin D1 (Ccnd1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. GreenT-EC - GPI

    Plasmid

    #193946

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Use

    Tags

    GPI-anchor and Igk_secretion - HA-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. GAL4-BRD4

    Plasmid

    #21943

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag and GAL4-DNA-BD

    Expression

    Bacterial

    Mutation

    aa 1209-1362

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

46. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. lentiMutB3GALT5catdead-blast

    Plasmid

    #220869

    Purpose

    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    encodes D156A and D243A mutations; also, nucleoti…

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLJM60 mUlk1 (wt) barcoded

    Plasmid

    #48799

    Purpose

    expresses wt Ulk1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    ULK1 (Ulk1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGATCC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

49. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMSCV-WT-mAbl

    Plasmid

    #69027

    Purpose

    Retroviral vector encoding WT mouse c-Abl

    Depositor

    Jean Wang

    Article

    Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

    Insert

    mouse Abl-Type IV (wild type)
    

    Use

    Retroviral

    Tags

    FLAG tag inserted at Internal SalI site (2934 bp)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only