We narrowed to 16,913 results for: igh

1. wtTDP43tdTOMATOHA

   Plasmid

   #28205

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   HA tag and tdtomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

   Plasmid

   #112925

   Purpose

   Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6 promoter, gRNA-SAM expression cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGM47523

   Plasmid

   #153221

   Purpose

   Level 0 zCas9i vector

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   SpCas9 with introns and 2 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TagBFP2-CAAX

   Plasmid

   #116856

   Purpose

   plasma membrane marker

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   HRAS
   

   Use

   Tags

   TagBFP2

   Expression

   Mammalian

   Mutation

   Amino acids 172-189

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pLX_311-Cas9

   Plasmid

   #96924

   Purpose

   for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

   Depositor

   John Doench, David Root, William Hahn

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   3xFlag and V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+-Tornado-split-nLuc

   Plasmid

   #212612

   Purpose

   For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-split-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   C373T mutation in CVB3

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. dClover2-C1

   Plasmid

   #54577

   Purpose

   Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

   Depositor

   Michael Davidson

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   dClover2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

   Plasmid

   #209727

   Purpose

   Expresses cytosolic medium affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95K.miRFP670nano3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Nme2Cas9_pMCSG7

    Plasmid

    #119925

    Purpose

    Nme2Cas9 bacterial expression plasmid

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    Tags

    NLS, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p11-LacY-wtx1

    Plasmid

    #69056

    Purpose

    The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

    Depositor

    Huimin Zhao

    Article

    Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

    Inserts

    ccdB (ccdB )
    lacY (lacY )
    

    Use

    Tags

    A177C

    Expression

    Mutation

    Promoter

    pBAD and pLac

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9 (PX165)

    Plasmid

    #48137

    Purpose

    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2-Cre.zf1

    Plasmid

    #61391

    Purpose

    mRNA synthesis of Cre recombinase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Cre.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_VASP_WH1

    Plasmid

    #109790

    Purpose

    Protein expression and purification of VASP_WH1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    VASP_WH1 (VASP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. dCas9-CRY2Clust

    Plasmid

    #200969

    Purpose

    induction of phase-separated condensate by CRY2Clust

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    CRY2Clust
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUbC-nls-ha-stdMCP-stdGFP

    Plasmid

    #98916

    Purpose

    synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

    Depositor

    Robert Singer

    Article

    Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

    Insert

    stdMCP-stdGFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. piggyBAC-H2B mCherry-XL (R125K)

    Plasmid

    #189926

    Purpose

    Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    CMV7

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Plasmid

    #58442

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSYNCMV tAKARα

    Plasmid

    #119921

    Purpose

    AAV construct with tAKARα being expressed under the human synapsin promotor with WPRE enhancer.

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209726

    Purpose

    Expresses cytosolic medium affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-PEAR-mCherry

    Plasmid

    #228892

    Purpose

    All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Insert

    EGFP-IRES-mScarlet
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBAD_TypeIC_Cascade_cas3_invivo

    Plasmid

    #196400

    Purpose

    expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

    Depositor

    David Taylor

    Article

    O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

    Inserts

    Cas5c
    Cas7c
    Cas8c
    Cas3c
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. modified pcAT7-Glo1

    Plasmid

    #160996

    Purpose

    Splicing reporter backbone

    Depositor

    Brenton Graveley

    Article

    Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

    Insert

    hemoglobin subunit beta (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Repeated intron 1 with 40 nucelotide deletion

    Promoter

    CAG

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRBC-sfGFP-150TAG

    Plasmid

    #174076

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Human J-chain

    Plasmid

    #158215

    Purpose

    Express the human J-chain

    Depositor

    Junyu Xiao

    Article

    Li et al Science. 2020 Feb 28;367(6481):1014-1017

    Insert

    JCHAIN (JCHAIN Human)
    

    Use

    Tags

    8×his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCH67 (crRNA expression vector)

    Plasmid

    #217337

    Purpose

    CROP-seq crRNA expression vector with GFP and puro marker

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    AsDR-filler-AsDR8, GFP-puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSCV-Myc-Caspase-5

    Plasmid

    #214305

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP5 (CASP5 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCytERM_mScarlet_N1

    Plasmid

    #85066

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    CytERM
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDB179-TurboPETase

    Plasmid

    #218693

    Purpose

    Encodes codon optimized TurboPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TurboPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Bxb1-GT_CAG-mRuby2

    Plasmid

    #229801

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRRL MND GFP

    Plasmid

    #36247

    Depositor

    David Rawlings

    Article

    Astrakhan et al Blood. 2012 Mar 19.

    Insert

    GFP (eGFP )
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    MND

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKI1.1R

    Plasmid

    #85808

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5A

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLOVE-miR302/367

    Plasmid

    #31310

    Depositor

    Ed Morrisey

    Article

    Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

    Insert

    mmu-miR302/367
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pLifeAct_mScarlet-i_N1

    Plasmid

    #85056

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPB EF1a CCR6-eLOV-TEVcs-Gal4

    Plasmid

    #173119

    Purpose

    Expresses CCR6 eLOV receiver construct in DIPG

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    CCR6-eLOV-TEVcs-Gal4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pH2B-miRFP703

    Plasmid

    #80001

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    histone cluster 1 H2B family member j (H2BC11 Human)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA flag PPAR gamma

    Plasmid

    #8895

    Purpose

    Mammalian expression of flag-tagged PPAR gamma

    Depositor

    Bruce Spiegelman

    Article

    Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge