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We narrowed to 90,874 results for: mal

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Showing: 9751 - 9800 of 90874 results

  6. pTFG005_Spike_Inducible_3xFlag

    Plasmid
    #191578
    Purpose
    Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Lentiviral
    Tags
    3x FLAG
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    191578-plasmid-map-sequence-id-442483

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  8. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

    Plasmid
    #188994
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…
    Promoter
    CMV
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188994-plasmid-map-sequence-id-371647

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  9. pDual-eGFP(H66)

    Plasmid
    #63558
    Purpose
    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(H66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pAM5188

    Plasmid
    #120087
    Purpose
    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    Use
    Other
    Available Since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120087-plasmid-map-sequence-id-234428

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  25. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  32. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available Since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47948-plasmid-map-sequence-id-68812

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  37. pTFG011_Spike_Parental_D614G_3xFlag

    Plasmid
    #191571
    Purpose
    SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G mutation. Last 19 amino acids truncated.
    Promoter
    CMV
    Available Since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    191571-plasmid-map-sequence-id-440477

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  39. pJZC78

    Plasmid
    #62339
    Purpose
    sgRNA + 1x COM with COM-KRAB effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 1x COM binding module
    COM-KRAB
    Use
    Lentiviral
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…
    Promoter
    CMV and U6
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62339-plasmid-map-sequence-id-102262

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  43. SpRYc

    Plasmid
    #175575
    Purpose
    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Article
    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:
    Insert
    SpRYc
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    PID of SpRY grafted onto the N-terminal domain of…
    Promoter
    CMV
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    175575-plasmid-map-sequence-id-345065

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  50. pJC49

    Plasmid
    #133060
    Purpose
    Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.
    Depositor
    Martin Kampmann
    Article
    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094
    Insert
    Tau.K18(LM)
    Use
    Lentiviral
    Tags
    mScarletI
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133060-plasmid-map-sequence-id-271174

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Showing: 9751 - 9800 of 90874 results