223,846 results

1. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-mDlx-GCaMP6f-Fishell-2 (AAV1)

   Viral Prep

   #83899-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-mDlx-GCaMP6f-Fishell-2 (#83899). In addition to the viral particles, you will also receive purified pAAV-mDlx-GCaMP6f-Fishell-2 plasmid DNA. mDlx-driven expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Promoter

   mDlx

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

3. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-BCR-ABL1-p190-IRES-EGFP

   Plasmid

   #219964

   Purpose

   Express human BCR-ABL1-P190 in mammalian cells

   Depositor

   Mohamed Fareh

   Insert

   BCR-ABL1-P190 (ABL1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKH116_keima-YIPF3

   Plasmid

   #214970

   Purpose

   Golgiphagy flux reporter keima-YIPF3

   Depositor

   Wade Harper

   Article

   Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

   Insert

   keima-YIPF3 (YIPF3 Human)
   

   Use

   Lentiviral

   Available Since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. En1

   Plasmid

   #34993

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Homeobox protein engrailed-1 (En1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. En1

   Plasmid

   #34993

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Homeobox protein engrailed-1 (En1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Peroxisomes-0

   Plasmid

   #54501

   Purpose

   Localization: Peroxisomes, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Type

   Empty backbone

   Tags

   EGFP and Peroximal Targeting Signal 1 (PTS1)

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. EGFP-Peroxisomes-0

   Plasmid

   #54501

   Purpose

   Localization: Peroxisomes, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Type

   Empty backbone

   Tags

   EGFP and Peroximal Targeting Signal 1 (PTS1)

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Flag‐mTim3

    Plasmid

    #49208

    Purpose

    expresses Flag tagged mouse Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (Havcr2 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    no Tim3 signal sequence

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag‐mTim3

    Plasmid

    #49208

    Purpose

    expresses Flag tagged mouse Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (Havcr2 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    no Tim3 signal sequence

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. STAT3-GFP

    Plasmid

    #110495

    Purpose

    reporter of STAT3 signaling activity

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    4 repeats of STAT3 binding site M67 at GFP promoter region
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHiUGE smFP-V5 donor ORF2

    Plasmid

    #200382

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+mNeonGreen-C Cloning Vector

    Plasmid

    #128144

    Purpose

    To express a protein of interest fused to the C-terminus of mNeonGreen

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    mNeonGreen fusion

    Expression

    Mammalian

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. DDX5 WT V5

    Plasmid

    #199576

    Purpose

    Expresses WT DDX5 cloned into pLenti puro

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    DDX5 (DDX5 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9 TadCBEd

    Plasmid

    #193835

    Purpose

    Express TadCBEd (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDd-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. hZMPSTE24-3FLAG_pQCXIP

    Plasmid

    #89759

    Purpose

    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

    Depositor

    Martin Dorf

    Article

    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

    Insert

    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    

    Use

    Retroviral; Bicistronic expression: puromycin re…

    Tags

    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

    Expression

    Mammalian

    Mutation

    Y253H in ZMPSTE24 (see depositor comments below)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBUE411-GGB

    Plasmid

    #198233

    Purpose

    Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411

    Depositor

    Andrea Gallavotti

    Inserts

    GRF4-GIF1 chimera cassette
    pPLTP::BBM cassette
    

    Use

    CRISPR; Plant binary vector

    Expression

    Plant

    Promoter

    Ubiquitin and maize PLTP

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEIGW roGFP2-Orp1

    Plasmid

    #64993

    Purpose

    Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEIGW roGFP2-Orp1

    Plasmid

    #64993

    Purpose

    Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPB_TRE3G_scFV-sfGFP-Ezh2FL

    Plasmid

    #235575

    Purpose

    Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMasterK

    Plasmid

    #165081

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    

    Use

    Episomal

    Expression

    Mammalian

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. psfaMs-Tn5

    Plasmid

    #183637

    Purpose

    Expresses anti-mouse nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti mouse secondary nanobody fused to Tn5
    

    Tags

    anti-mouse nanobody

    Expression

    Bacterial

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCDNA6A-EGFR WT

    Plasmid

    #42665

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR (EGFR Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCDNA6A-EGFR WT

    Plasmid

    #42665

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR (EGFR Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti CMV/TO Hygro empty (w214-1)

    Plasmid

    #17484

    Purpose

    3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti CMV/TO Hygro empty (w214-1)

    Plasmid

    #17484

    Purpose

    3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Human SERCA2a (pcDNA3.1+)

    Plasmid

    #75187

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    David MacLennan, Jonathan Lytton

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. Human SERCA2a (pcDNA3.1+)

    Plasmid

    #75187

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    David MacLennan, Jonathan Lytton

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV human IRS-1 CAAX

    Plasmid

    #11238

    Depositor

    Ronald Kahn

    Article

    Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

    Insert

    IRS-1 (IRS1 Human)
    

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    C-terminal prenylation motif makes IRS-1 localize…

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

31. pCMV human IRS-1 CAAX

    Plasmid

    #11238

    Depositor

    Ronald Kahn

    Article

    Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

    Insert

    IRS-1 (IRS1 Human)
    

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    C-terminal prenylation motif makes IRS-1 localize…

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

32. MAC3-tag-C

    Plasmid

    #185481

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/UltralD

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Type

    Empty backbone

    Tags

    StrepIII-HA-UltraID

    Expression

    Bacterial and Mammalian

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

    Plasmid

    #101060

    Purpose

    AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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34. pCAX-EGFP-CIP4

    Plasmid

    #178363

    Purpose

    Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Cdc42 Interacting Protein 4 (TRIP10 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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35. mito-EcSTH

    Plasmid

    #211921

    Purpose

    Mitochondrial localization of EcSTH

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    mito-EcSTH
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pK036.TRE-Flpe-WPRE (Supernova)

    Plasmid

    #85007

    Purpose

    For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    NLS-FLPe-WPRE
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pK036.TRE-Flpe-WPRE (Supernova)

    Plasmid

    #85007

    Purpose

    For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    NLS-FLPe-WPRE
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGreenPuro-shRNA-Stx3S-C4

    Plasmid

    #99742

    Purpose

    3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

    Insert

    shRNA against Stx3S
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge