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163,421 results

Showing: 8201 - 8240 of 163421 results

  9. TRE3G-PE2-P2A-BFP

    Plasmid
    #231580
    Purpose
    Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein
    Depositor
    Jesse Engreitz
    Article
    Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.
    Insert
    PE2-P2A-BFP
    Use
    Lentiviral
    Tags
    P2A-BFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231580-plasmid-map-sequence-id-462114

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  10. pRSET.HaloTag.v7.AviTag

    Plasmid
    #195486
    Purpose
    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.
    Depositor
    HHMI-JRC Tool Translation Team, Tim Brown
    Insert
    HaloTag.v7.AviTag
    Use
    Tags
    6xHIS and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195486-plasmid-map-sequence-id-393193

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  15. pAAV-hSyn-hM3D(Gq)-mCherry (AAV1)

    Viral Prep
    #50474-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  17. 3xFlag-Rab34

    Plasmid
    #232458
    Purpose
    Expresses 3xFlag-tagged human Rab34 in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    Rab34 (RAB34 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232458-plasmid-map-sequence-id-463631

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  20. pAAV-Ef1a-mCherry (AAV8)

    Viral Prep
    #114470-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  25. pAAV-CAG-GFP (AAV1 trial size)

    Viral Prep
    #37825-AAV1.T
    Purpose
    Ready-to-use AAV1 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only

  29. rAAV2/cMG

    Plasmid
    #184539
    Purpose
    AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid
    Depositor
    Minmin Luo
    Article
    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s
    Insert
    AAV-cMG capsid
    Use
    AAV; Helper vector for packaging
    Tags
    Expression
    Mutation
    changes on AAV9 capsid: K449R, A589D; insertion o…
    Promoter
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184539-plasmid-map-sequence-id-361936

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  30. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132393-plasmid-map-sequence-id-341619

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  33. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  34. pMLS7

    Plasmid
    #32056
    Purpose
    Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria
    Depositor
    Miguel Valvano
    Article
    Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ps7 promoter, originated from Paraburkholderia xe…
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    32056-plasmid-map-sequence-id-220094

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  36. pGfa(B3)-nLac

    Plasmid
    #53132
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of the gfa2 B segment…
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53132-plasmid-map-sequence-id-82721

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Showing: 8201 - 8240 of 163421 results