163,421 results

1. pcdna 3.1 DSG-3 Tension Sensor

   Plasmid

   #182717

   Purpose

   expresses DSG-3 TSmod

   Depositor

   Daniel Conway

   Article

   Jin et al bioRxiv [Preprint]. 2024 May 6:2024.05.0

   Insert

   desmoglein-3 (Dsg3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TSmod inserted between G726 and G727

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZac2.1-GfaABC1D-Rpl22-HA

   Plasmid

   #111811

   Purpose

   Express Rpl22HA in astrocytes

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Ribosomal protein L22 (Rpl22 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MET-HYGRO

   Plasmid

   #218113

   Purpose

   Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LYS-Leu2

   Plasmid

   #218114

   Purpose

   Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMK289 (mAID-mClover-NeoR)

   Plasmid

   #72827

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Neo
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF5/FRT/DEST-3xHA/mGli2

   Plasmid

   #51250

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pPEE13

   Plasmid

   #128359

   Purpose

   Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mCherry ORF and a flexable linker at the 5' end.

   Depositor

   James Fraser

   Article

   Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

   Insert

   Acetamidase (amdS)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR-HOT-P2A-Clover-BlastR

   Plasmid

   #138568

   Purpose

   Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   P2A-clover-BlastR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW13.lentiguide.mCherry

   Plasmid

   #104375

   Purpose

   mCherry vector for cloning in guides

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid

    #212022

    Purpose

    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GianCreg (GOLGB1 Human)
    

    Use

    Tags

    td5StayGold(c4)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXLE-mCherry

    Plasmid

    #193293

    Purpose

    episomal vector expressing mCherry fluorescent protein

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TFORF2910

    Plasmid

    #143665

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFIC (NFIC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF2912

    Plasmid

    #143667

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFIC (NFIC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSMALB-ATF4.5

    Plasmid

    #155032

    Purpose

    Fluorescent reporter for ATF4 translation

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

    Plasmid

    #113691

    Purpose

    U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-YFP

    Plasmid

    #11180

    Purpose

    Mammalian expression vector for expression of YFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    EYFP from Aequorea victoria
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)-RIα-EGFP

    Plasmid

    #181840

    Purpose

    GFP-tagged PKA RIα subunit for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV1)

    Viral Prep

    #50476-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    CaMKII

    Tags

    None

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

19. pDONR223_CRY1_WT

    Plasmid

    #82264

    Purpose

    Gateway Donor vector containing CRY1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CRY1 (CRY1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2 GW delCMV

    Plasmid

    #29496

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hCRISPRa-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/gene

    Pooled Library

    #83981

    Purpose

    Human CRISPRa Pooled Library targeting cancer and apoptosis genes

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

22. Integrin β4-GFP

    Plasmid

    #205092

    Purpose

    Express GFP-tagged human integrin β4 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 4 (ITGB4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HA, EGFP Spastin M87 with exon 4

    Plasmid

    #167542

    Purpose

    For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4.

    Depositor

    Tarun Kapoor

    Article

    Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

    Insert

    Spastin M87 with exon 4 (SPAST Human)
    

    Use

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAGM47523

    Plasmid

    #153221

    Purpose

    Level 0 zCas9i vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    SpCas9 with introns and 2 NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO-PTEN-shRNA-3001

    Plasmid

    #25639

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-3001 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FLAG-HSF1

    Plasmid

    #32537

    Depositor

    Stuart Calderwood

    Article

    Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    CMV

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MG1655 ΔendA ΔrecA

    Bacterial Strain

    #37853

    Depositor

    Kristala Prather

    Article

    Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

    Bacterial Resistance

    None

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pETSXM2-pLacI-mtrC

    Plasmid

    #174615

    Purpose

    Bacterial expression of mtrC from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    MtrC
    

    Use

    Synthetic Biology; Shuttle vector in e. coli and …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pME-mVenus-CAAX

    Plasmid

    #75153

    Purpose

    Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mVenus-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ202

    Plasmid

    #86198

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis ubiquitin 10 (AtUbi10)

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGRNA2e

    Plasmid

    #169782

    Purpose

    Insert one gRNA

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGRNA1

    Plasmid

    #169778

    Purpose

    Insert one gRNA

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR221-SLC39A12_STOP

    Plasmid

    #161295

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A12 (SLC39A12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

    Plasmid

    #51248

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907,980,1006 mutated to A…

    Promoter

    EF1a

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

35. pOpen-T7gene1

    Plasmid

    #165505

    Purpose

    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    T7 RNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pBS-3xpA

    Plasmid

    #176505

    Purpose

    3x SV40 polyA sequences

    Depositor

    Mario Capecchi

    Insert

    3x SV40 polyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Arf1-GFP

    Plasmid

    #39554

    Depositor

    Paul Melancon

    Article

    Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

    Insert

    ADP ribosylation factor 1 (ARF1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDONR221-SLC30A5_STOP

    Plasmid

    #161243

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A5 (SLC30A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pDONR221-SLC30A6_STOP

    Plasmid

    #161231

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A6 (SLC30A6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. TFORF2883

    Plasmid

    #144255

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BHLHA9 (BHLHA9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge