161,255 results
-
Plasmid#85152Purposeacute activation of Rho through rapamycin-induced translocation to membrane-localized FRBDepositorInsertFusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-NLS-HA
Plasmid#141321PurposePlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)DepositorInsertRfx-Cas13d (NLS-RfxCas13d-NLS)
UseCRISPRTagsHA and NLSExpressionMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-mBaoJin-oPRE
Plasmid#218683PurposeAAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInsertmBaoJin
UseAAVTagsExpressionMammalianMutationPromoterHuman synapsin promoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
px458_2A_GFP_sgRNA_RACK1
Plasmid#127123DepositorInsertgRNA RACK1 (RACK1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-rtetTAK8N, L131L
Plasmid#165968PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-NDP52
Plasmid#187828PurposePlasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586DepositorInsertNDP52 (CALCOCO2 Human)
UseTagsGST-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
mDsRed-C1
Plasmid#54502PurposeLocalization: C1 Cloning Vector, Excitation: 557, Emission: 585DepositorTypeEmpty backboneUseTagsmDsRedExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
p15aC-1D
Plasmid#107863PurposeBacterial expression of PISDepositorInsertPIS
UseTagsmycExpressionBacterialMutationPromoterproDAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
TERT - R865C
Plasmid#213926PurposeExpresses Mutant TERT R865CDepositorInsertMutant TERT R865C (TERT Human)
UseLentiviralTags6x His TagExpressionMammalianMutationArginine 865 changed to CysteinePromoterTet-responsive promoter PTight, consisting of sev…Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-KDM6A
Plasmid#203580PurposeExpresses wild-type KDM6A in mammalian cells.DepositorInsertKDM6A (KDM6A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-(-2)AP-Ub
Plasmid#232582PurposeExpress Ubiquitin tagged with a modified Acceptor Peptide (AP)DepositorInsertUbiquitin
UseTagsAPExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEHAC1-HA-BirAonly
Plasmid#232584PurposeExpress HA-BirADepositorInsertBirA
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-AP-Ub-dGG
Plasmid#232581PurposeExpress Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycinesDepositorInsertUbiquitin
UseTagsAviTagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-AP(-3)-Ub
Plasmid#232583PurposeExpress Ubiquitin tagged with a modified Acceptor Peptide (AP)DepositorInsertUbiquitin
UseTagsAPExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-AP-Ub
Plasmid#232577PurposeExpress Ubiquitin tagged with an Acceptor Peptide (AP)DepositorInsertUbiquitin
UseTagsAviTagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEHAC1-CHIP-GSGS-BirA-HA
Plasmid#232588PurposeExpress BirA tagged to the C-terminus of CHIP with a GSGS linker between BirA and CHIP.DepositorInsertCHIP - BirA (CHIP Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
Plasmid#198417PurposeExpresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Arl13b-mCardinal
Plasmid#194542PurposeExpresses Arl13b with mCardinal in mammalian cells and zebrafish embryosDepositorInsertArl13b (Arl13b Mouse)
UseTagsmCardinalExpressionMammalianMutationPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV puro Tet VSVG
Plasmid#82724PurposeLentiviral plasmid that expresses VSVG protein under a doxycycline-inducible promoterDepositorInsertVSVG
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8s-WPRE (AAV5)
Viral Prep#162374-AAV5PurposeReady-to-use AAV5 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-dsRedExpress
Plasmid#65032PurposeGmR mini-Tn7 vector, mobilizable, for RFP tagging bacteriaDepositorInsertdsRedExpress
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
pUC57-CmYLCV
Plasmid#205245PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8m-WPRE (AAV Retrograde)
Viral Prep#176751-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaTagsNoneAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1
Plasmid#205443Purposehuman AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55DepositorInserthCD46-HLA-G SCT-hCD47-hCD59
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS2 (hANGPTL4-V5)
Plasmid#102446PurposeExpresses V5-tagged human ANGPTL4 in mammalian cellsDepositorInsertANGPTL4 (ANGPTL4 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
loSIIN
Plasmid#185663PurposeshApc-expressing lentiviruses with varius levels of expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
GFP
UseLentiviralTagsExpressionMutationPromoterEf1aAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS162
Plasmid#194485PurposesynGR CassetteDepositorInsertsynGR Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305
Plasmid#41390PurposeConstitutive lentiviral expression, SV40-puro; PGK-gateway-no tagDepositorTypeEmpty backboneUseLentiviral; Gateway destination vectorTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-zSpRY
Plasmid#179317PurposeIn vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoterDepositorInsertZebrafish codon-optimized Cas9 variant SpRY
UseCRISPR; In vitro transcription by t3 promoterTagsSV40-NLSExpressionMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…PromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human Lipid Droplet and Metabolism Library
Pooled Library#191535PurposeLentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-OPTN WT
Plasmid#190191PurposePlasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797DepositorInsertoptineurin (OPTN Human)
UseTags6xHis, TEV, and mCherryExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Blast
Plasmid#227254PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.21.5
Plasmid#98203PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI Probe
Plasmid#211507PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteriaDepositorInsertBcPI-PLC-ANH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNA-binding protein pooled CRISPR knockout library
Pooled Library#141438PurposeCRISPR knockout library targets RBPs with 10 sgRNAs/geneDepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM mouse islet1 [TJ#28]
Plasmid#16270DepositorInsertislet1 (Isl1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSH624-ssr
Plasmid#199241PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertsingle-stranded DNA recombineering
UseTagsExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only