Addgene

We narrowed to 2,785 results for: 2-Oct

Showing: 1051 - 1100 of 2785 results

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pDEST40-2XHA-Src-Y530F

Plasmid

#140318

Purpose

Expresses HA-tagged human Src-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Y530F

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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FEMP

Plasmid

#191973

Purpose

FRET-based indicator of ER-mitochondria proximity (FEMP)

Depositor

Luca Scorrano

Article

Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

AkapYFP-Tav2a-CFP-sac1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-wtSrc

Plasmid

#140294

Purpose

Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAV5B+longBRD4

Plasmid

#157792

Purpose

Expression of long isoform of BRD4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

long BRD4 (BRD4 Human)

Use

Tags

His6

Expression

Insect

Mutation

Promoter

polyhedron

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJP-HBB-nLuc

Plasmid

#175431

Purpose

Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nLUC plus 5' HBB leader

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-K298M

Plasmid

#140314

Purpose

Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE DN Cullin2

Plasmid

#41912

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL2 (CUL2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-jGCaMP7b-XC

Plasmid

#178361

Purpose

Improved genetic encoded calcium sensor based on GCaMP7b.

Depositor

Xiaodong Liu

Article

Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE DN Cullin4A

Plasmid

#41914

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-2XFL-Src-K298M

Plasmid

#140315

Purpose

Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETduet+sfGFP-TetR-p300HAT

Plasmid

#157795

Purpose

Expression of sfGFP-TetR-p300HAT fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR-p300 (EP300 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin1

Plasmid

#41911

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL1 (CUL1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-puro GSK3 beta (S9A)

Plasmid

#14128

Depositor

Brendan Manning

Article

Zhang et al Mol Cell. 2006 Oct 20. 24(2):185-97.

Insert

GSK3 beta (S9A) (GSK3B Human)

Use

Retroviral

Tags

V5 tag

Expression

Mammalian

Mutation

S9A. Mutant version of GSK3 that lacks the inhibi…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-DEPTOR-WT

Plasmid

#159159

Purpose

Expresses DEPTOR in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
peCALWY-1

Plasmid

#22233

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-1 Zn(II) sensor (ATOX1 Human, GFP's)

Use

Tags

Expression

Mammalian

Mutation

linker consists of 9 GGSGGS repeats

Promoter

Available since

Nov. 18, 2009

Availability

Academic Institutions and Nonprofits only
pDEST40-2XHA-Src-Y419F

Plasmid

#140316

Purpose

Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Y419F

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV CaMKIIa iChloC 2A tDimer

Plasmid

#66709

Purpose

Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

Depositor

Thomas Oertner

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E83Q,E90R,E101S,D156N,T159C

Promoter

CaMKIIa

Available since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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peCALWY-6

Plasmid

#22238

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY6 Zn(II) sensor (ATOX1 Human, GFP's)

Use

Tags

Expression

Mammalian

Mutation

C416S mutation Identical to peCALWY-4, but linke…

Promoter

Available since

Jan. 5, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5-ALK6-HA

Plasmid

#11769

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK6 (Bmpr1b Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
peCALWY-5

Plasmid

#22237

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-5 Zn(II) sensor (ATOX1 Human, GFP's)

Use

Tags

Expression

Mammalian

Mutation

C416S mutation identical to peCALWY-4, but linke…

Promoter

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
peCALWY-3

Plasmid

#22235

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-3 Zn(II) sensor (ATOX1 Human, GFP's)

Use

Tags

Expression

Mammalian

Mutation

Similar to eCALWY-1, but with a linkerlength of 3…

Promoter

Available since

Nov. 20, 2009

Availability

Academic Institutions and Nonprofits only
pDEST40-2XFL-Src-Y419F

Plasmid

#140317

Purpose

Expresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

Y419F

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-2XFL-Src-Y530EEI

Plasmid

#140323

Purpose

Expresses Flag-tagged human Src-Y530EEI in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

Y530EEI

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES260-sc.eEF2

Plasmid

#85119

Purpose

yeast expression of EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSFG-TFII-I-GFP beta

Plasmid

#22200

Depositor

Ananda Roy

Article

Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-Lin28A-mCCHC

Plasmid

#51372

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28A (LIN28A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

CCHC domain mutant (H147A, H169A)

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
ATF4 14: 5’ATF4.uORF1&2AUA.GFP

Plasmid

#21861

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

AUG codons of uORFs1&2 were converted to AUA

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRa34_1384

Plasmid

#49693

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF34_1384

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pR-BAM

Plasmid

#22649

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1L (Myt1l Rat)

Use

Cloning and rna synthesis

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
KHBD00158

Plasmid

#34312

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG33183 (Hr46 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pLJM1-zeo-ORF66-2xStrep

Plasmid

#130957

Purpose

Lentiviral vector for expression of KSHV ORF66 with a C-terminal 2xStrep tag

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Lentiviral

Tags

2xStrep

Expression

Mutation

Promoter

CMV

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRa38_1322

Plasmid

#49698

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF38_1322

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRa27_4265

Plasmid

#49682

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF27_4265

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00327

Plasmid

#34450

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG18783 (Kr-h1 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pBAD-HisD-BR1

Plasmid

#192816

Purpose

Expresses BR1 in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-2XHA-Src-Y530EEI

Plasmid

#140322

Purpose

Expresses HA-tagged human Src-Y530EEI in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Y530EEI

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRIME-CMV-GFP-shNts2

Plasmid

#132714

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shNts(2) (Nts Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-zeo-ORF30-2xStrep

Plasmid

#130958

Purpose

Lentiviral vector for expression of KSHV ORF30 with a C-terminal 2xStrep tag

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF30 (ORF30 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Lentiviral

Tags

2xStrep

Expression

Mutation

Promoter

CMV

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-zeo-ORF24-3xFLAG

Plasmid

#130959

Purpose

Lentiviral vector for expression of KSHV ORF24 with a C-terminal 3xFLAG tag

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF24 (ORF24 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

CMV

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES260-sc.eEF2 K509A

Plasmid

#85120

Purpose

yeast expression of mutant EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K509A

Promoter

GAL1

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mei-S332 5.6Kb genomic DNA in Casper4

Plasmid

#112984

Purpose

P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo) (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC45

Plasmid

#104819

Purpose

CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.04g057400

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRa17_1458

Plasmid

#49671

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF17_1458

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRa14_1324

Plasmid

#49665

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF14_1324

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
spCas9-BlastR (pCBhCas9-BlastR)

Plasmid

#71489

Purpose

Expresses SpCas9 and Blasticidin resistance

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

spCas9

Use

Tags

Expression

Mutation

Promoter

CBh

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_ionRD

Plasmid

#172931

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_ionRE

Plasmid

#172932

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pmVirD2-GFP

Plasmid

#90315

Purpose

mVirD2-GFP expression plasmid

Depositor

Bjorn Hogberg

Article

Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

Insert

A. tumefaciens VirD2 from aa 1 to 204 - GFP

Use

Tags

6xHis and GFP

Expression

Bacterial

Mutation

Promoter

T7/T5 IPTG inducible

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETA_ionRD

Plasmid

#172924

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRD

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETA_ionRE

Plasmid

#172926

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRE

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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