161,697 results

1. pJMP3

   Plasmid

   #79875

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into thrC

   Depositor

   Carol Gross, Stanley Qi, Jason Peters

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCerulean3-C1

   Plasmid

   #54605

   Purpose

   Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Type

   Empty backbone

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti-RfxCas13d-Blast-BFP

   Plasmid

   #196726

   Purpose

   Sortable and selectable RfxCas13d.

   Depositor

   Bernhard Schmierer

   Insert

   RfxCas13d-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDIRE

   Plasmid

   #26745

   Depositor

   Rolf Zeller

   Article

   Osterwalder et al Nat Methods. 2010 Oct 17

   Insert

   iCre expression cassette
   

   Use

   Cre/Lox; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mTagRFP-Membrane-1

   Plasmid

   #57992

   Purpose

   Localization: Plasma Membrane, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   N-terminal 20 Aas MLS from Neuromodulin and mTagR…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. ERM-APEX2

   Plasmid

   #79055

   Purpose

   Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 anchored to the cytosolic face of the ER membrane
   

   Use

   Lentiviral

   Tags

   Residues 1-27 of P450 oxidase 2C1 and V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV immearly promotor

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. hKCNQ5(WT).ires.GFP-pcDNA3

   Plasmid

   #204360

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   KCNQ5 (KCNQ5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7 RNApol

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_Xph20-eGFP-CCR5TC

   Plasmid

   #135530

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGH125_dCas9-Blast

   Plasmid

   #85417

   Purpose

   lentiviral expression vector for dCas9 with Blasticidin selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   dCas9 and Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. His-GFP-Syntaxin1A

    Plasmid

    #170871

    Purpose

    Mammalian expression of His-GFP-Syntaxin1A.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Syntaxin 1A (Stx1a Mouse)
    

    Use

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. plenti-UbcP-HA-CRBN-pGK-HYG

    Plasmid

    #107378

    Purpose

    Lentiviral expression of HA-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UbcP

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT-HA-hM4D(Gi)

    Plasmid

    #45548

    Purpose

    Expresses hM4D (Gi) neuronal inhibitor

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    hM4D (Gi)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

    Plasmid

    #204971

    Purpose

    Soma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    raACR
    

    Use

    AAV

    Tags

    mCerulean3

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

    Plasmid

    #163047

    Purpose

    GCaMPer calcium sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHBV-Luc

    Plasmid

    #71414

    Purpose

    To see the activity of HBV transcription using Luciferase assay

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

    Insert

    HBV core promoter, enhancer I, enhancer II and luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HBV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP2-NbALFA

    Plasmid

    #201486

    Purpose

    BRET2 detection of ALFA-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    GFP2-NbALFA
    

    Use

    Tags

    GFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid

    #52628

    Purpose

    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency

    Depositor

    Scot Wolfe

    Insert

    Kanamycin cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

    Plasmid

    #132404

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-MCN-10His-StayGold

    Plasmid

    #211360

    Purpose

    Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    StayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry_a_tubulin_IRES_puro2

    Plasmid

    #21043

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-STAT2

    Plasmid

    #71451

    Purpose

    3rd generation lentiviral expression of STAT2

    Depositor

    George Stark

    Article

    Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

    Insert

    STAT2 (STAT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMU-PLAr

    Plasmid

    #61200

    Purpose

    Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtRubisco1sp-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pK031.TRE-Cre (Supernova)

    Plasmid

    #69136

    Purpose

    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

    Depositor

    Takuji Iwasato

    Article

    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

    Insert

    NLS-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid

    #103870

    Purpose

    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSLHDR03_mCherry_tCD19_unique guide

    Plasmid

    #124062

    Purpose

    Construction of 1st donor for scarless genome editing with unique guide

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Inserts

    mCherry
    tCD19
    

    Use

    Mouse Targeting; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Human UbC modified

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-CMV-KIF2C

    Plasmid

    #205994

    Purpose

    Over-expression of GFP-KIF2c

    Depositor

    Floris Foijer

    Article

    Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

    Insert

    KIF2c (KIF2C Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-syn-ChR2-EYFP-Kv

    Plasmid

    #89256

    Purpose

    Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Insert

    Channelrhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. R-pre-GFP

    Plasmid

    #17274

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2006 Jul 21. 313(5785):347-51.

    Insert

    R-pre
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2008

    Availability

    Academic Institutions and Nonprofits only

29. pGFPHYG2

    Plasmid

    #30173

    Depositor

    Lalita Ramakrishnan

    Article

    Cosma et al Infect Immun. 2006 Jun . 74(6):3125-33.

    Insert

    Mycobacterium Strong Promoter (MSP)
    

    Use

    Mycobacteria expression

    Tags

    GFP

    Expression

    Mutation

    GFPmut3 allele has improved brightness.

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pM1s3AsG

    Plasmid

    #137921

    Purpose

    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    Superfolder GFP
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-FLAG-MTOR-L1460P

    Plasmid

    #69006

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-L1460P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Leucine 1460 to Proline

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV_BiLAMP5e3_GCaMP6f

    Plasmid

    #213917

    Purpose

    AAV construct expressing GCaMP6f driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_GCaMP6f

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pInducer-myc-ERK2-R67S/S153D

    Plasmid

    #213597

    Purpose

    Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R67S, S153D

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAG364-greenFAST

    Plasmid

    #160353

    Purpose

    expresses greenFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    greenFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FlucDM-HA-GFP11-N1

    Plasmid

    #91956

    Purpose

    Expresses aggregation-prone FlucDM-HA-GFP11 in mammalian cells

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    FlucDM-HA-GFP11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R188Q,R261Q

    Promoter

    CMV

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EF05J

    Plasmid

    #42941

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    Negative control Functionally Dead Human L1 element (L1RE1 Human)
    

    Use

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    ORF1 R261A, R262A

    Promoter

    Available since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

38. His-AgdNK

    Plasmid

    #203226

    Purpose

    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EGFP-C3-beta-spectrin

    Plasmid

    #202685

    Purpose

    fluorescent protein tagged beta-spectrin

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    EGFP:SPTB(1-2364) (SPTB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196084

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRK-flag-GFP11

    Plasmid

    #78590

    Purpose

    Expression flag-GFP 11 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-GFP 11
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

42. Flag-HA-COPS5

    Plasmid

    #22541

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    COPS5 (COPS5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pKP332 (Lenti-OSK1)

    Plasmid

    #21627

    Depositor

    Tim Townes

    Article

    Chang et al Stem Cells. 2009 May . 27(5):1042-9.

    Insert

    hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Human, Porcine teschovirus-1)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCHW90004

    Plasmid

    #222436

    Purpose

    Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. TFORF1066

    Plasmid

    #143919

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFATC2 (NFATC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pDEST47-Arl13b12

    Plasmid

    #40872

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    no stop codon

    Promoter

    CMV

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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47. pRSF-G1TFAKRS

    Plasmid

    #177311

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3.1 His-NLRP1 codon-optimized

    Plasmid

    #166833

    Purpose

    Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Nsp3 -EGFP

    Plasmid

    #165108

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp3 (ORF1ab SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. Syn61Δ3(ev5) ΔrecA (ev1)

    Bacterial Strain

    #189857

    Purpose

    Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

    Depositor

    George Church

    Article

    Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Synthetic

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only