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176,210 results

Showing: 11201 - 11250 of 176210 results

  1. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100982-plasmid-map-sequence-id-392326

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  9. pSBtet-RN

    Plasmid
    #60503
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60503-plasmid-map-sequence-id-97544

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  11. pDEST51-LRRK2-G2019S

    Plasmid
    #29401
    Depositor
    Mark Cookson
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Gateway Cloning
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Gly2019Ser
    Available Since
    April 1, 2011
    Availability
    Academic Institutions and Nonprofits only
    29401-plasmid-map-sequence-id-256667

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  14. p3E_he1a:eYFP

    Plasmid
    #113879
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    eYFP
    Available Since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113879-plasmid-map-sequence-id-222563

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  15. pVP144

    Plasmid
    #222081
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi
    Insert
    dCas9-PmCDA1, sfGFP, sacB and eforRED
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222081-plasmid-map-sequence-id-438346

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  19. pTriEx-SAMBA

    Plasmid
    #236390
    Purpose
    A new chemically induced proximity (CIP) tool using salicylic acid or aspirin after hydrolysis as the small molecule trigger
    Depositor
    Yubin Zhou
    Article
    Wang et al Nat Chem Biol. 2025 Jun 13. doi: 10.1038
    Insert
    Evolved from NtNPR1 GENE
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    236390-plasmid-map-sequence-id-472854

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  20. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

    Plasmid
    #187459
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187459-plasmid-map-sequence-id-367469

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  25. pCAG-N2cN

    Plasmid
    #100801
    Purpose
    express rabies virus CVS N2c strain nucleoprotein gene (N2cN)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain nucleoprotein gene
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    100801-plasmid-map-sequence-id-189511

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  27. mito-meGFP

    Plasmid
    #172481
    Purpose
    meGFP targeted to the mitochondrial matrix with a human COX8 presequence
    Depositor
    Thomas Schwarz
    Insert
    Mito
    Tags
    meGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    172481-plasmid-map-sequence-id-345344

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  28. pcDNA3.1-TRPV1exCellHalo

    Plasmid
    #220307
    Purpose
    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices
    Depositor
    Eric Senning
    Article
    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231
    Insert
    rTRPV1exCellHalo (Trpv1 Rat)
    Tags
    circularly permutated Halotag (Deo et al., Nat Ch…
    Expression
    Mammalian
    Mutation
    linker included to insert cpHaloTag (
    Promoter
    CMV promoter
    Available Since
    July 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220307-plasmid-map-sequence-id-436649

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  32. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50587-plasmid-map-sequence-id-75659

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  38. pZac2.1-GfaABC1D-lck-jGCaMP8s (AAV1)

    Viral Prep
    #176761-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8s (#176761). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8s plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.n into animals.
    Depositor
    Loren Looger
    Promoter
    GfaABC1D
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  45. pNL[NlucP/NFAT-RE/Hygro] Vector

    Plasmid
    #236929
    Purpose
    Express NFAT response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    NFAT
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236929-plasmid-map-sequence-id-474673

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Showing: 11201 - 11250 of 176210 results