179,553 results
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Pooled library#159392PurposeMouse lentiviral genome-wide CRISPR libraryDepositorExpressionMammalianSpeciesMus musculusUseCRISPR and LentiviralAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCAG R2pH-LAMP1-3xFLAG
Plasmid#157940Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
Tags3XFLAG, mCherry, and pHluorinExpressionMammalianPromoterChick beta actin pomoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-PGK-Venus-Fluc (puro)
Plasmid#140328PurposeLentivirus for expression of Venus-Fluc (firefly luciferase) fusion proteinDepositorInsertVenus-Firefly Luciferase (luc2)
UseLentiviralPromoterhuman PGK promoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP
Plasmid#154786PurposeExpresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBEDepositorInsertAdenosine deaminse (Adar Fly, Synthetic)
UseLentiviralTagsGFP, V5, and mCherryExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEE515
Plasmid#149288PurposeT-DNA encoding TRV2 complete genome with MCSDepositorTypeEmpty backboneExpressionPlantAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCSDest-HA-TMPRSS2
Plasmid#154963PurposeTMPRSS2 with N-terminal HADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHA-TMPRSS2 (TMPRSS2 Human)
TagsHAExpressionMammalianAvailable sinceJuly 14, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pAAV-Ef1a-Con/Fon-mCherry
Plasmid#137132PurposeIntersectional viral expression of mCherry in cells expressing both Cre AND FlpDepositorHas serviceAAV8InsertCon/Fon-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-Pa1
Plasmid#138475PurposeEnables high-efficiency recombineering in P. aeruginosaDepositorInsertsPapRecT
mutL E36K
ExpressionBacterialMutationE36KAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tet_pLKO.1_puro_shNRF2 #2
Plasmid#136585PurposeExpresses an inducible short hairpin targeting human NRF2 sequenceDepositorAvailable sinceJune 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (AAV8)
Viral prep#50467-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (#50467). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine plasmid DNA. CaMKIIa-driven HA-tagged hM4D(Gi) and bicistronic mCitrine expression, for chemogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCitrineAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-Bst-LF
Plasmid#145799PurposePlasmid for the aTc-inducible expression of the large-fragment of Bst DNAPDepositorInsertBst-LF
Tags6xHisExpressionBacterialPromotertet PAAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
p3xFLAG CMV14 / BRAF-V600E
Plasmid#131723PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
Plasmid#128424PurposeHuman Codon Optimized dCas9-DNMT3A3LDepositorInsertdCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
UseCRISPR and LentiviralTagsGFPExpressionMammalianPromoterT7 PromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sgRNA Ef1alpha Puro-T2A-GFP
Plasmid#111596PurposesgRNA Ef1alpha Puro-T2A-GFPDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pci-EGFP-UPF1-WT
Plasmid#135994PurposeWT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUPF1
TagsEGFPExpressionMammalianAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-6Xhis-human SUMO 3
Plasmid#133772Purposeexpress 6Xhis- SUMO 3 proteinDepositorAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-CaM
Plasmid#127393PurposeFluorescent reporter for calmodulin 1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCalmodulin 1 (Calm1 Mouse)
TagsmCherryExpressionMammalianPromoterpCAGAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pB-EF1a-NLuc-IRES-Puro
Plasmid#130936PurposePiggyBac vector for constitutive NanoLuc expression in mammalian cellsDepositorInsertNanoLuc
ExpressionMammalianPromoterEF1aAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCC001
Plasmid#124451PurposeMulti-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targetsDepositorInsertHH-BsaI
ExpressionYeastMutationgRNA expression cassette from pUDP002 modified to…PromoterTDH3 promoter from S.cerevisiae (already present …Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-CAG-FLEX-BFP2-(mCherry)’
Plasmid#115234PurposeLentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after itDepositorInsertmTagBFP2 and mCherry
ExpressionMammalianAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H2B-iRFP720
Plasmid#128961PurposeLentiviral plasmid for expression of far red iRFP720 nuclear markerDepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsiRFP720ExpressionMammalianPromoterhPGKAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
spisPink chromoprotein
Plasmid#117839PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coliDepositorInsertpromoter, RBS, spisPink
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Strep-KIFC1*-mCh
Plasmid#120169PurposeExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertkinesin family member C1 (KIFC1 Human)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIFC1: 125-673 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBT280
Plasmid#122610Purposeexpress evoFERNY-BE4max in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertevoFERNY-BE4max
ExpressionMammalianMutationH102P D104NAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTrcHIS-wt7D12
Plasmid#125268PurposeExpresses wild type 7D12 anti-EGFR nanobodiesDepositorInsert7D12 nanobody
TagsHis-TagExpressionBacterialPromoterTrcAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CROPseq-Guide-Zeo
Plasmid#127173PurposeEntry plasmid for guide cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1 hMOG-alpha1-EGFP
Plasmid#126463PurposeExpression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyelin oligodendrocyte glycoprotein (MOG Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-CMV-mC-G-LC3B-P
Plasmid#124974Purposeevaluate autophagyDepositorInsertmCherry-GFP-LC3B
UseLentiviralPromoterCMVAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET29b-AK1-His
Plasmid#124135PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-ihSyn1-DIO-tTA (AAV1)
Viral prep#99121-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-ihSyn1-DIO-tTA (#99121). In addition to the viral particles, you will also receive purified pAAV-ihSyn1-DIO-tTA plasmid DNA. Cre-dependent expression of the tet-off transactivator from an inducible synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterihSyn1Available sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Floxed-STOP Fluc
Plasmid#122962PurposeFirefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reactionDepositorInsertpcDNA3.1_Floxed-STOP Fluc
UseCre/Lox and LuciferaseExpressionMammalianPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymScarletI-CaURA3
Plasmid#118457PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection. *An updated version of this plasmid is available: #168055 pFA6a-link-ymScarletI-URA3.DepositorInsertymScarletI
ExpressionYeastAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EasyCloneYALI- Integrative Vector Set
Plasmid kit#1000000140PurposeIntegrative plasmids for overexpression of heterologous genes in Yarrowia lipolytica.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector typeYeast ExpressionCloning typeUSERAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-AK1-His
Plasmid#118977PurposeBacterial expression plasmid for Adenylate kinase isoenzyme 1DepositorInsertadenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
Tags6x-HisExpressionBacterialPromoterT7 promoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRE-Tn7-152
Plasmid#118566PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn7 delivery vectorExpressionBacterialAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-eYFP
Plasmid#117382PurposeAn AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoterDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV RetrogradeInsertEYFP
UseAAVExpressionMammalianPromoterhSyn1Available sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LX-miR
Pooled library#112200PurposeTargets 1,594 primary human miRNA genes. The library contains 4-5 gRNAs per gene and 1,000 non-targeting control gRNAs for a total of 8,382 gRNAs.DepositorExpressionMammalianSpeciesHomo sapiensUseLentiviralAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-betaCatenin
Plasmid#114281PurposeTET-inducible expression of a constitutively active form of the BetaCatenin proteinDepositorAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-tdTomato-WPRE
Plasmid#104112PurposeAAV-mediated expression of tdTomato under the TRE promoter.DepositorInserttdTomato
UseAAVPromoterTREAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TIWB-yellow pre-eGRASP(p32)
Plasmid#111582PurposeAn AAV vector that expresses yellow pre-eGRASP(p32) under the tetO promoter.DepositorInsertyellow pre-eGRASP(p32)
UseAAVPromotertetOAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV_ABEmax_3xHA
Plasmid#112098PurposeA:T-to-G:C base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABEmax_3xHA
UseCRISPRTags3xHAExpressionMammalianPromoterCMVAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40
Plasmid#105551PurposeAAV expression of GFP-Cre from CamKII promoterDepositorHas serviceAAV9 and AAV RetrogradeInsertEGFP-Cre
UseAAV and Cre/LoxExpressionMammalianPromoterCamKIIAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNO286-3
Plasmid#107746PurposeKey genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)DepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialMutationDeleted AA 223 and 512PromoterJ23106 and J23108Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.GFA104.PI.eGFP.WPRE.bGH (AAV5)
Viral prep#100896-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.GFA104.PI.eGFP.WPRE.bGH (#100896). In addition to the viral particles, you will also receive purified pAAV.GFA104.PI.eGFP.WPRE.bGH plasmid DNA. GFA104-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFA104 (GFAP promoter)TagsEGFPAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUNO mouse CD274 + 3'UTR WT full length
Plasmid#107012PurposeExpresses murine PD-L1 with the 3'UTRDepositorInsertCD274 (Cd274 Mouse)
ExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-nEF-dCas9
Plasmid#106430PurposeExpresses SpdCas9 from the nEF promoter in AAV backboneDepositorInsertnEF-dCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianPromoternEFAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
Tags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389Available sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT2-Flag
Plasmid#102623PurposeExpresses full length SIRT2 isoform 1 in mammalian cellsDepositorAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TRE3G-SOX17
Plasmid#104541PurposePiggyBac vector encoding doxycycline-inducible human SOX17DepositorAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40
Plasmid#101061PurposeAAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas serviceAAV1InsertCaMPARI2-F391W
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by