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171,853 results

Showing: 11201 - 11250 of 171853 results

  1. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Available Since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92073-plasmid-map-sequence-id-275942

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  2. pTP943

    Plasmid
    #104157
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fab specific nanobody TP886
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104157-plasmid-map-sequence-id-200077

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  3. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Expression
    Bacterial and Mammalian
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

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  4. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Expression
    Bacterial and Mammalian
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

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  7. pJL 89

    Plasmid
    #98558
    Purpose
    Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.
    Depositor
    John Lindbo
    Article
    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.
    Type
    Empty backbone
    Expression
    Plant
    Promoter
    Enh 35S
    Available Since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98558-plasmid-map-sequence-id-192654

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  16. TFORF3034

    Plasmid
    #144510
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    OTX1 (OTX1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144510-plasmid-map-sequence-id-404226

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  17. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit
    #1000000156
    Purpose
    Library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.
    Depositor
    Scott Lenaghan
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only

  22. H2B-Grx1-roGFP2

    Plasmid
    #236550
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    H2B-Grx1-roGFP2 (H2BC21 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236550-plasmid-map-sequence-id-469939

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  23. PB-TA-ERN

    Plasmid
    #80474
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80474-plasmid-map-sequence-id-151580

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  24. Ultra-Exon1Q145-Myc-A

    Plasmid
    #110487
    Purpose
    Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Partial long 3'UTR wild type huntingtin exon…
    Promoter
    UbC
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110487-plasmid-map-sequence-id-212608

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  30. pBabe-Kras Wt

    Plasmid
    #75282
    Purpose
    Expresses human Kras wild-type
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    None. Wild-Type
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    75282-plasmid-map-sequence-id-299863

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  36. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)

    Viral Prep
    #100853-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  41. pCK306

    Plasmid
    #110544
    Purpose
    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021
    Inserts
    PrhaBAD
    yfp
    rhaS
    Expression
    Bacterial
    Promoter
    N/A, PrhaBAD, and kanR promoter (upstream of kanR…
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110544-plasmid-map-sequence-id-212164

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  43. IDG_CLCC1_OE_1

    Plasmid
    #161672
    Purpose
    Overexpresses CLCC1
    Depositor
    Mike McManus
    Insert
    CLCC1 (CLCC1 Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    161672-plasmid-map-sequence-id-319664

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  46. pAAV.GFAP.eGFP.WPRE.hGH (AAV5)

    Viral Prep
    #105549-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GFAP.eGFP.WPRE.hGH (#105549). In addition to the viral particles, you will also receive purified pAAV.GFAP.eGFP.WPRE.hGH plasmid DNA. GFAP-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    GFAP
    Tags
    EGFP
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  47. pcDNA3-Cyto-ExRai-CKAR2

    Plasmid
    #236097
    Purpose
    Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    ExRai-CKAR2-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236097-plasmid-map-sequence-id-473746

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  50. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available Since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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Showing: 11201 - 11250 of 171853 results