Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

179,553 results

Showing: 11251 - 11300 of 179553 results

  1. pGL4-AARE-luc2P-Hygro

    Plasmid
    #101787
    Purpose
    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)
    Depositor
    Seiichi Oyadomari
    Publication
    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xAARE
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    minimal TATA-box promoter with low basal activity
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    101787-plasmid-map-sequence-id-194135

    Enlarge

  9. pl-Synapsin-YFP-NaVII-III

    Plasmid
    #91246
    Purpose
    Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)
    Depositor
    Matthew Grubb
    Publication
    Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.
    Insert
    Nav1.2 (Scn2a Rat)
    Use
    Lentiviral
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    The insert codes only for the intracellular loop …
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91246-plasmid-map-sequence-id-180624

    Enlarge

  12. AICSDP-13:FBL-mEGFP

    Plasmid
    #87427
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FBL Homology Arms with linker-mEGFP (FBL Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles
    87427-plasmid-map-sequence-id-173980

    Enlarge

  14. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid
    #72255
    Purpose
    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre-P2A-tdTomato-T2A-Puro
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  20. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Expression
    Yeast
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83475-plasmid-map-sequence-id-358352

    Enlarge

  22. pLV_Zika_NS2B_Flag

    Plasmid
    #79637
    Purpose
    Third generation Self-inactivating lentivector expressing NS2B from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS2B
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    79637-plasmid-map-sequence-id-151178

    Enlarge

  28. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid
    #75141
    Purpose
    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells
    Depositor
    Bruce Beutler
    Publication
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    fragment encoding amino acids 711-1034
    Promoter
    CMV
    Available since
    May 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    75141-plasmid-map-sequence-id-148062

    Enlarge

  31. pCMV6 SMCR8 HA

    Plasmid
    #74158
    Purpose
    Express SMCR8 HA tagged in mammalian cells
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    SMCR8 (SMCR8 Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74158-plasmid-map-sequence-id-139169

    Enlarge

  32. pKHR7

    Plasmid
    #74624
    Purpose
    a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.
    Depositor
    David Grunwald
    Publication
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cmlc2::mCherry
    Use
    Zebrafish expression
    Promoter
    cmlc2
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    74624-plasmid-map-sequence-id-204495

    Enlarge

  34. pMZ379

    Plasmid
    #74215
    Purpose
    Combined sgRNA/Cas9 vector with NatMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    74215-plasmid-map-sequence-id-139305

    Enlarge

  40. pEF_dCas9

    Plasmid
    #68416
    Purpose
    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    68416-plasmid-map-sequence-id-120194

    Enlarge

  41. GCGR-Tango

    Plasmid
    #66291
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCGR (GCGR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles

  44. Beta-2-adrenergic receptor-YFP

    Plasmid
    #67146
    Purpose
    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-2-adrenergic receptor-YFP (ADRB2 Human, Aequorea victoria)
    Tags
    YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    67146-plasmid-map-sequence-id-121580

    Enlarge

  45. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    66080-plasmid-map-sequence-id-436984

    Enlarge

  47. pcDNA3-TORCAR-NLS

    Plasmid
    #64931
    Purpose
    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TORCAR-NLS (EIF4EBP1 Synthetic, Human)
    Tags
    Cerulean, Nuclear localization signal (NLS), and …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64931-plasmid-map-sequence-id-109697

    Enlarge

  48. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

    Plasmid kit
    #1000000062
    Purpose
    Plasmids to create all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs for genome engineering using dCas9 and FokI-dCas9. The Multiplex CRISPR/Cas9 Assembly System Kit (kit #1000000055) is required for the use of this accessory pack.
    Depositor
    Takashi Yamamoto
    Application
    Genome Editing
    Vector type
    Mammalian Expression
    Editing type
    CRISPR
    Available since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. GFP-ATAD2

    Plasmid
    #65370
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATAD2 (ATAD2 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65370-plasmid-map-sequence-id-177053

    Enlarge

Showing: 11251 - 11300 of 179553 results