Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

290,447 results

Showing: 11251 - 11300 of 290447 results

  6. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Expression
    Mammalian
    Promoter
    Pcmv
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

    Enlarge

  7. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Expression
    Mammalian
    Promoter
    Pcmv
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

    Enlarge

  18. OPRM1-DuET

    Plasmid
    #213361
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRM1 (OPRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213361-plasmid-map-sequence-id-431826

    Enlarge

  20. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

    Enlarge

  26. dCas9-CBP core

    Plasmid
    #179543
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179543-plasmid-map-sequence-id-354769

    Enlarge

  27. dCas9-CBP core

    Plasmid
    #179543
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179543-plasmid-map-sequence-id-354769

    Enlarge

  28. pCB-1

    Plasmid
    #210387
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210387-plasmid-map-sequence-id-418472

    Enlarge

  36. pJYS1Ptac

    Plasmid
    #85545
    Purpose
    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter
    Depositor
    Sheng Yang
    Article
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Expression
    Bacterial
    Promoter
    tac and unknown
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85545-plasmid-map-sequence-id-176966

    Enlarge

  37. pJYS1Ptac

    Plasmid
    #85545
    Purpose
    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter
    Depositor
    Sheng Yang
    Article
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Expression
    Bacterial
    Promoter
    tac and unknown
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85545-plasmid-map-sequence-id-176966

    Enlarge

  48. pNvT-MHC::mCh

    Plasmid
    #67943
    Purpose
    Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI
    Depositor
    Ulrich Technau
    Article
    Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107
    Insert
    mCherry
    Use
    Transgenic reporter in the sea anemone, nematoste…
    Promoter
    Myosin Heavy Chain1 (MyHC1)
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67943-plasmid-map-sequence-id-266110

    Enlarge

  49. pNvT-MHC::mCh

    Plasmid
    #67943
    Purpose
    Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI
    Depositor
    Ulrich Technau
    Article
    Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107
    Insert
    mCherry
    Use
    Transgenic reporter in the sea anemone, nematoste…
    Promoter
    Myosin Heavy Chain1 (MyHC1)
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67943-plasmid-map-sequence-id-266110

    Enlarge

  50. 458 p5E-uasE1b

    Plasmid
    #26026
    Depositor
    Nathan Lawson
    Insert
    E1B basal promoter + UAS repeats
    Use
    Entry vector
    Available Since
    Aug. 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    26026-plasmid-map-sequence-id-12211

    Enlarge

Showing: 11251 - 11300 of 290447 results