We narrowed to 16,609 results for: IGH@

1. pHH0103_VCP_AAA

   Plasmid

   #109964

   Purpose

   Protein expression and purification of VCP_AAA

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   VCP_AAA (VCP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT2ADW-lox-mCherryNLS-pA-lox-4493NES

   Plasmid

   #138374

   Purpose

   Booster-PKA for the generation of mice

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   lox-mCherry-lox-Booster-PKA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-deSpCas9-VP64-6xHis

   Plasmid

   #92117

   Purpose

   Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSFDuet-1_VNp-FGF21

   Plasmid

   #182407

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-fgf21 (FGF21 Human)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-CMV-DNAJC5 (L115R)

   Plasmid

   #205728

   Purpose

   Mammalian expression of DNAJC5 (L115R)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (L115R) (DNAJC5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ143-ZmUbi

   Plasmid

   #138107

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_PLCG1_SH3

   Plasmid

   #110035

   Purpose

   Protein expression and purification of PLCG1_SH3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PLCG1_SH3 (PLCG1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX6P1-Ubl-sr1(SIRPT1)

   Plasmid

   #75298

   Purpose

   Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

   Depositor

   Kalle Gehring

   Article

   Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

   Insert

   Human Sacsin 1-339
   

   Use

   Tags

   GST-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221_SLC6A2

   Plasmid

   #131891

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Sijben et al Sci Rep. 2021 Jun 10;11(1):12290. doi: 1

   Insert

   SLC6A2 (SLC6A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pHH0103_GNA11_G-alpha

    Plasmid

    #109911

    Purpose

    Protein expression and purification of GNA11_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNA11_G-alpha (GNA11 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX SHP-1(C)-SH2

    Plasmid

    #46494

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(C) (PTPN6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 105-217

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAGGS-Flex/3'USS(Luc)-GFP

    Plasmid

    #197887

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ265G2

    Plasmid

    #164723

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-ziSpyMac(D10A)-UGI)
    

    Use

    CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKT2/CAGXSP/PalmNanoLuc

    Plasmid

    #182969

    Purpose

    Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

    Insert

    PalmNanoLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBT3035

    Plasmid

    #122543

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_lmn-1::CemOrange2 (lmn-1 Synthetic, Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmScarlet-H_Giantin_C1

    Plasmid

    #85049

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo

    Plasmid

    #179439

    Purpose

    Expresses rTetR-KRAB(human) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCKO_PSMD1_sgRNA_5

    Plasmid

    #74181

    Purpose

    lentiviral vector expressing sgRNA targeting PSMD1

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    PSMD1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCR2.1-promoter-mIL-6

    Plasmid

    #109296

    Purpose

    IL-6 promoter reporter construct

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    murine IL-6 promoter (Il6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter in pEGFP-N1 replaced with murine IL-…

    Promoter

    murine IL-6 promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Crk-SH2

    Plasmid

    #46418

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Crk (CRK Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-124

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

    Plasmid

    #163698

    Purpose

    AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

    Depositor

    Gina Turrigiano

    Article

    Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

    Insert

    CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII fragment

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-CaMKIIa-ChRmine-eYFP-Kv2.1-WPRE

    Plasmid

    #130989

    Purpose

    AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    EYFP-Kv2.1

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVC297 VEGF Site#1

    Plasmid

    #47505

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 1 (VEGFA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHH0103_AURKB_Pkinase

    Plasmid

    #110025

    Purpose

    Protein expression and purification of AURKB_Pkinase

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    AURKB_Pkinase (AURKB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLCKO_PSMD1_sgRNA_1

    Plasmid

    #74180

    Purpose

    lentiviral vector expressing sgRNA targeting PSMD1

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    PSMD1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVC228 VEGF Site#3

    Plasmid

    #47507

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 3 (VEGFA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Clover-TOMM20-N-10

    Plasmid

    #56307

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

28. mRuby2-TOMM20-N-10

    Plasmid

    #55913

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

29. pGEX p85a(C)-SH2

    Plasmid

    #46463

    Depositor

    Bruce Mayer, Leszek Kotula

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85a(C) (PIK3R1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 612-724

    Promoter

    Tac

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_AKT1_Pkinase

    Plasmid

    #109775

    Purpose

    Protein expression and purification of AKT1_Pkinase

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    AKT1_Pkinase (AKT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX Fes-SH2

    Plasmid

    #46426

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Fes (FES Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 396-497

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Kif1A(1-489)-DHFR-myc

    Plasmid

    #117833

    Purpose

    Constitutively active Kinesin-3 motor tagged with DHFR and myc

    Depositor

    Thomas Schwarz

    Article

    Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

    Insert

    Kif1A (1-489) (Kif1a Mouse)
    

    Use

    Tags

    DHFR and myc

    Expression

    Mutation

    only aa 1-489 (motor domain)

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. NES-EGFP-cPHx1

    Plasmid

    #116853

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 )
    

    Use

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    Amino acids 169.329

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHH0103_RPA2_RPA-C

    Plasmid

    #109795

    Purpose

    Protein expression and purification of RPA2_RPA-C

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RPA2_RPA-C (RPA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

    Plasmid

    #210504

    Purpose

    expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28-mTDGa.1

    Plasmid

    #81051

    Purpose

    bacterial expression of catalytically dead murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    asparagine 151 changed to alanine

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHH0103_KPNB1_HEAT

    Plasmid

    #110017

    Purpose

    Protein expression and purification of KPNB1_HEAT

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    KPNB1_HEAT (KPNB1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-Elafin

    Plasmid

    #18102

    Depositor

    Ronny Drapkin

    Article

    Labidi-Galy et al Oncogene. 2015 Jan 15;34(3):373-83. doi:

    Insert

    Elafin (PI3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

39. mRuby2-Actin-C-18

    Plasmid

    #55889

    Purpose

    Localization: Actin, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pAG247

    Plasmid

    #33200

    Depositor

    David Bartel

    Article

    Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

    Insert

    LRIG1 (LRIG1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early enhancer/promoter

    Available since

    Jan. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

41. pBAD mApple

    Plasmid

    #91772

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    monomeric Apple
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    R97K, E152S, A180S, V207T compared to mApple (FP …

    Promoter

    araBAD

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mOrange2-Fibrillarin-7

    Plasmid

    #57957

    Purpose

    Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pGEX p85a(N)-SH2

    Plasmid

    #46464

    Depositor

    Bruce Mayer, Leszek Kotula

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85a(N) (PIK3R1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 321-434

    Promoter

    Tac

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGEMHE-mH33B-mClover3

    Plasmid

    #171496

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H3.3B (H3f3b Mouse)
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pSL007_PB_pCMV-H2B-mIFP-T2A-rTetR-ratKRAB-zeo

    Plasmid

    #179438

    Purpose

    Expresses rTetR-KRAB(rat) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pET28a_6HT-TBP6.7(R49A)

    Plasmid

    #112726

    Purpose

    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R49A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R49A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pGEX SHP-1(N)-SH2

    Plasmid

    #46495

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(N) (PTPN6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-109

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLCKO_EIF3D_sgRNA_1

    Plasmid

    #74187

    Purpose

    lentiviral vector expressing sgRNA targeting EIF3D

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    EIF3D sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. Puro-TTL-wt-TTF-1

    Plasmid

    #67657

    Purpose

    To create a dox-on inducible expression of human wt-TTF-1 cDNA in cells

    Depositor

    David Mu

    Article

    Rice et al J Biol Chem. 2013 Jun 7;288(23):16348-60

    Insert

    Thyroid Transcription Factor 1 (NKX2-1 Human)
    

    Use

    Retroviral

    Tags

    N/A

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLCKO_EIF3D_sgRNA_4

    Plasmid

    #74188

    Purpose

    lentiviral vector expressing sgRNA targeting EIF3D

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    EIF3D sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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