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Showing: 441 - 460 of 67199 results

  1. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid
    #91796
    Purpose
    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.
    Depositor
    James Bradner, Behnam Nabet
    Publication
    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP_F36V-2xHA-P2A-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Phenylalanine 36 to valine in FKBP12
    Promoter
    Promotorless
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91796-plasmid-map-sequence-id-334366

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  5. pEP4 E02S EM2K

    Plasmid
    #20923
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4
    Depositor
    James Thomson
    Publication
    Yu et al Science. 2009 Mar 26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
    Use
    Episomal/ebna
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20923-plasmid-map-sequence-id-272720

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  10. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Publication
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63155-plasmid-map-sequence-id-220487

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  12. pcDNA-dCas9

    Plasmid
    #47106
    Purpose
    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    Flag and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive)
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47106-plasmid-map-sequence-id-433987

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  20. eeBxb1

    Plasmid
    #222339
    Purpose
    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells
    Depositor
    David Liu
    Publication
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    222339-plasmid-map-sequence-id-473210

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Showing: 441 - 460 of 67199 results