Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

165,803 results

Showing: 9281 - 9320 of 165803 results

  4. pAAV-nEF-Coff/Fon-ChR2-mCherry (AAV8)

    Viral Prep
    #137144-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2-mCherry (#137144). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Flp-dependent expression of ChR2-mCherry for optogenetic activation (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    mCherry (Flp-dependent)
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only

  13. pSBtet-Hyg

    Plasmid
    #60508
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60508-plasmid-map-sequence-id-97539

    Enlarge

  16. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

    Enlarge

  21. The Mammalian Toolkit

    Plasmid Kit
    #1000000180
    Purpose
    A ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.
    Depositor
    Hana El-Samad
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only

  23. DR274

    Plasmid
    #42250
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription
    Depositor
    Keith Joung
    Article
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR; Zebrafish expression
    Tags
    Expression
    Worm
    Mutation
    Promoter
    T7
    Available Since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    42250-plasmid-map-sequence-id-254852

    Enlarge

  24. pSEVA63_Helper

    Plasmid
    #190432
    Purpose
    M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)
    Depositor
    Manish Kushwaha
    Article
    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.
    Insert
    M13 genes: I-XI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    190432-plasmid-map-sequence-id-485074

    Enlarge

  28. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (AAV9)

    Viral Prep
    #112005-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and mRuby3 expression. The mRuby3 will be a separate protein (not a fusion protein). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.
    Promoter
    Syn
    Tags
    mRuby3
    Available Since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only

  30. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

    Enlarge

  34. pLenti.PGK.LifeAct-Ruby.W

    Plasmid
    #51009
    Purpose
    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-mRFPruby
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51009-plasmid-map-sequence-id-339916

    Enlarge

  35. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

    Plasmid
    #216668
    Purpose
    Expresses membrane-bound mCherry in mammalian cells
    Depositor
    Leonardo Morsut
    Insert
    mCherry
    Use
    Tags
    PDGFR Transmembrane Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216668-plasmid-map-sequence-id-432187

    Enlarge

  36. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep
    #154868-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available Since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  38. pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC

    Plasmid
    #221363
    Purpose
    Mammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC; to be used with Anti-His [IPI-HAP1/5.13.22] light chain (Plasmid 221364) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221363-plasmid-map-sequence-id-436831

    Enlarge

  39. pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa

    Plasmid
    #221364
    Purpose
    Mammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa; to be used with Anti-His [IPI-HAP1/5.13.22] heavy chain (Plasmid 221363) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221364-plasmid-map-sequence-id-436832

    Enlarge

Showing: 9281 - 9320 of 165803 results