We narrowed to 27,701 results for: flag

1. pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

   Plasmid

   #172627

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. attB53-Pac-TRE-3xFlag-Neurog1-attB53

   Plasmid

   #183613

   Purpose

   RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   Neurog1 (Neurog1 Mouse)
   

   Use

   Rmce

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP

   Plasmid

   #99731

   Purpose

   overexpression of PDGFRa and CopGFP

   Depositor

   Mengsheng Qiu

   Article

   Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

   Insert

   Pdgfra (Pdgfra Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB-CAG-3xFLAG-empty-pgk-hph

   Plasmid

   #48754

   Purpose

   PiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tag

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

   Plasmid

   #90010

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Cdc25C WT
   NBirA*-14-3-3e
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

   Plasmid

   #81022

   Purpose

   Cancer-associated ASXL1 truncation mutant in retroviral vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   p.Y591X truncation

   Promoter

   5’LTR

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

   Plasmid

   #163988

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   M1457A

   Promoter

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKS031 - pCAGGS-3XFLAG-BORIS-eGFP-bpA

   Plasmid

   #156443

   Purpose

   For transient expression of mouse BORIS (CTCFL) tagged with eGFP

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse BORIS (Ctcfl Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-TAT-1-101-FLAG basic mutant

   Plasmid

   #35985

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV1, 101 aa; R to A mutations at aa 56,…

   Promoter

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

    Plasmid

    #176060

    Purpose

    Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K164A and H165A

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Flag YAP127/128/131/381A

    Plasmid

    #27378

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    S127/128/131/381A

    Promoter

    Available since

    March 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

    Plasmid

    #172618

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr283Ala

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

    Plasmid

    #166804

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S1213A

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB-CAG-3xFLAG::Flcn-pgk-hph

    Plasmid

    #48757

    Purpose

    PiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    3xFLAG::Flcn (Flcn Mouse)
    

    Use

    Piggybac

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

    Plasmid

    #176059

    Purpose

    Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

    Plasmid

    #133789

    Purpose

    CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized CjeCas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWZL Hygro-NFLAG TRF1 deltaA deltaM

    Plasmid

    #16297

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted acidic domain (aa1-66) and myb domain (aa…

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

    Plasmid

    #156439

    Purpose

    For transient expression of mouse PDS5A tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse PDS5A (Pds5a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAd-Track Flag GCN5 Y621A/P622A

    Plasmid

    #14425

    Depositor

    Pere Puigserver

    Article

    Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

    Insert

    GCN5 Y621A/P622A (KAT2A Human)
    

    Use

    Adenoviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Y621A, P622A. Catalytically inactive acetyltransf…

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pLVX-Tight-Puro FLAG-SMAD4 TetON

    Plasmid

    #107129

    Purpose

    Inducible expression of Flag-tagged SMAD4 in mammalian cells

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    SMAD4
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 1357 pcDNA3 flag FKHR AAA delta DB

    Plasmid

    #10696

    Depositor

    William Sellers

    Insert

    FKHR AAA deltaDB (FOXO1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    T24A S256A S319A , deletion in aa 208-220

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

22. hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

    Plasmid

    #42650

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV/TO

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L

    Plasmid

    #122991

    Purpose

    Expresses Flag-tagged hRPGRIP1L in mammalian cells

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Insert

    RPGRIP1L (RPGRIP1L Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

    Plasmid

    #156442

    Purpose

    For transient expression of mouse PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse PDS5B (Pds5b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BPK1169: CAG-DmrC-NLS-3xFLAG-P65

    Plasmid

    #104564

    Purpose

    Mammalian expression vector for DmrC fused to p65 activation domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    DmrC-p65
    

    Use

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. IFI16-(192-393)-HinA (pcDNA3-FLAG)

    Plasmid

    #35062

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Contains HinA domain: aa 192-393

    Promoter

    CMV

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

27. IFI16-(515-710)-HinB (pcDNA3-FLAG)

    Plasmid

    #35063

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(515-710)-HinB (IFI16 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains HinB domain: aa 515-710

    Promoter

    CMV

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

    Plasmid

    #163990

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1461R

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXS-IRES-BLAST Halo-flag-CAD

    Plasmid

    #188117

    Purpose

    Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag and Halo tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR-SFFV-FLAG-TurboID-mutKIR-SQSTM1

    Plasmid

    #223719

    Purpose

    Expresses FLAG-TurboID-mutKIR-SQSTM1

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Use

    Lentiviral

    Tags

    TurboID

    Expression

    Mammalian

    Mutation

    Mutation 347-DPSTGE-352 to 347-APSAAA-352

    Promoter

    SFFV

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1

    Plasmid

    #223716

    Purpose

    Expresses FLAG-TurboID-dPB1-SQSTM1

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Use

    Lentiviral

    Tags

    TurboID

    Expression

    Mammalian

    Mutation

    deletion 1-119

    Promoter

    SFFV

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1

    Plasmid

    #223717

    Purpose

    Expresses FLAG-TurboID-dUBA-SQSTM1

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Use

    Lentiviral

    Tags

    TurboID

    Expression

    Mammalian

    Mutation

    deletion 384-Stop codon

    Promoter

    SFFV

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pHR-SFFV-FLAG-TurboID-mutLIR-SQSTM1

    Plasmid

    #223718

    Purpose

    Expresses FLAG-TurboID-mutLIR-SQSTM1

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Use

    Lentiviral

    Tags

    TurboID

    Expression

    Mammalian

    Mutation

    Mutation 335-DDDW-338 to 335-AAAA-338

    Promoter

    SFFV

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid

    #198569

    Purpose

    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed glutamic acid E164 to lysine (E164K)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

    Plasmid

    #69049

    Purpose

    lentiviral expression of IKZF2 variant 2 with FLAG tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF2 (IKZF2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    CAG

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

    Plasmid

    #53551

    Purpose

    mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"

    Depositor

    Kathleen Collins

    Article

    Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    contains aa 88-544, with aa 166-172 replaced wit…

    Promoter

    CMV

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127315

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

    Plasmid

    #32008

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    Lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

    Promoter

    CMV, SP6

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p6346 MSCV-CMV-Flag-HA-Brd4-1-444

    Plasmid

    #32886

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

    Plasmid

    #69842

    Purpose

    This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    K41M-S285A-T289A

    Promoter

    CMV

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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42. pcDNA3 Flag FKHR H215R AAA mutant

    Plasmid

    #13510

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Promoter

    Available since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

43. 5'-3xFLAG Chtop-exon minimal CMV pCDNA5

    Plasmid

    #212080

    Purpose

    LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Chtop-exon (Chtop Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

    Plasmid

    #204547

    Purpose

    Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deltaUBA

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

    Plasmid

    #90014

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-TNRC6C
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. Flag-TRIM24-C840W/F869D/F979A/N980A

    Plasmid

    #28144

    Depositor

    Michelle Barton

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    C840W, F869D, F979A, N980A

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

47. pLenti-PGK-flag-RAP1B-V12-puro

    Plasmid

    #156168

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

    Plasmid

    #134331

    Purpose

    CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized Nme2Cas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

    Plasmid

    #175421

    Purpose

    Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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