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165,803 results

Showing: 9481 - 9520 of 165803 results

  3. siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)

    Plasmid
    #226578
    Purpose
    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen
    Depositor
    Gia Voeltz
    Article
    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382
    Insert
    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    F196A, L199A, F200A, L203A, Y206A, L210A, L214A, …
    Promoter
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    226578-plasmid-map-sequence-id-451909

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  15. TFORF3196

    Plasmid
    #144672
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZIC1 (ZIC1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144672-plasmid-map-sequence-id-350525

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  16. pcDNA3.1-ccdB-3xFLAG

    Plasmid
    #214984
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214984-plasmid-map-sequence-id-440018

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  17. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV9)

    Viral Prep
    #26975-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

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  20. P2RY6-Tango

    Plasmid
    #66476
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    P2RY6 (P2RY6 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pJYDNg

    Plasmid
    #162452
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Ha and myc and eUnaG2 fluorescent protein
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162452-plasmid-map-sequence-id-321450

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  24. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

    Plasmid
    #193356
    Purpose
    VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193356-plasmid-map-sequence-id-379848

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  25. biGBac Kit

    Plasmid Kit
    #1000000088
    Purpose
    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.
    Depositor
    Jan-Michael Peters
    Available Since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. TFORF0320

    Plasmid
    #141582
    Purpose
    Lentiviral vector for overexpressing the MSLN transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MSLN (MSLN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141582-plasmid-map-sequence-id-396940

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  32. pBT1-proD-mCherry

    Plasmid
    #65823
    Purpose
    Const. mCherry expression , proD promoter, E. coli Expression
    Depositor
    Michael Lynch
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    proD E . coli Constitutive Promoter
    Available Since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65823-plasmid-map-sequence-id-111984

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  35. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  37. pENN.AAV.CMVs.Pl.Cre.rBG (AAV8)

    Viral Prep
    #105537-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    None
    Available Since
    Dec. 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  40. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid
    #225756
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Article
    Walton et al bioRxiv 2024.03.17.585235
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225756-plasmid-map-sequence-id-446064

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Showing: 9481 - 9520 of 165803 results