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169,450 results

Showing: 9481 - 9520 of 169450 results

  1. pGreenFire1_E2F1RE

    Plasmid
    #112248
    Purpose
    expression of GFP and Luciferase under the control of E2F1 responsive element
    Depositor
    Simone Di Giovanni
    Insert
    E2F1RE (responsive element)
    Use
    Lentiviral
    Tags
    copGFP and luciferase
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112248-plasmid-map-sequence-id-213659

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  4. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

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  6. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

    Plasmid
    #188994
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…
    Promoter
    CMV
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188994-plasmid-map-sequence-id-371647

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  8. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Expression
    Bacterial
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

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  14. pMito-MtrxRFP2

    Plasmid
    #172324
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV, SP6
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172324-plasmid-map-sequence-id-431104

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  15. TFORF2199

    Plasmid
    #141967
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RXRA (RXRA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    141967-plasmid-map-sequence-id-395884

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  22. pC1-CMV-mito-DAAO

    Plasmid
    #141132
    Purpose
    Expresses DAAO with mitochondrial localization signal in mammalian cells
    Depositor
    Vsevolod Belousov
    Insert
    mito-DAAO
    Tags
    2 mitochondrial localization signals and 7xHis
    Expression
    Mammalian
    Mutation
    deleted DAAO amino acids 367-368
    Promoter
    CMV
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    141132-plasmid-map-sequence-id-323598

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  24. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  25. BII-Sh-FUCCI

    Plasmid
    #133363
    Purpose
    Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    Clover-Geminin-IRES-mKO2-Cdt1
    Tags
    HA-tagged Clover-Geminin
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133363-plasmid-map-sequence-id-267621

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  27. pINDUCER20_mStrawberry_Atg4BC74A

    Plasmid
    #161735
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy
    Depositor
    Alec Kimmelman
    Article
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mStrawberry
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Available Since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161735-plasmid-map-sequence-id-320115

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  28. pLV_Zika_NS3_Flag

    Plasmid
    #79635
    Purpose
    Third generation Self-inactivating lentivector expressing NS3 from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS3
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79635-plasmid-map-sequence-id-152697

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  31. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Promoter
    constitutive 35SPPDK promoter
    Available Since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52256-plasmid-map-sequence-id-116013

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  33. xlox(GFP)hTERT

    Plasmid
    #69809
    Purpose
    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a GFP marker
    Depositor
    David Ott
    Insert
    hTERT Reverse Transcriptase (TERT )
    Use
    Retroviral
    Tags
    none
    Promoter
    MLV promoter
    Available Since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    69809-plasmid-map-sequence-id-127377

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  34. eGFP-ova-C1

    Plasmid
    #163524
    Purpose
    Expresses both eGFP and chicken ovalbumin in mammalian cells
    Depositor
    Thomas Weber
    Insert
    enhanced-GFP-chicken ovalbumin tandem
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163524-plasmid-map-sequence-id-321577

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  36. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Article
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    170069-plasmid-map-sequence-id-333408

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Showing: 9481 - 9520 of 169450 results