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161,255 results

Showing: 921 - 960 of 161255 results

  2. pAAV-hSyn-hChR2(H134R)-EYFP (AAV9)

    Viral Prep
    #26973-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    EYFP
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only

  3. pcDNA3/F-tractin=mStayGold

    Plasmid
    #212019
    Purpose
    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    F-tractin
    Use
    Tags
    mStayGold
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212019-plasmid-map-sequence-id-419223

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  4. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV9)

    Viral Prep
    #100845-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Tags
    None
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. L4440

    Plasmid
    #1654
    Depositor
    Andrew Fire
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    RNAi
    Tags
    T7p, T7p, lacZN, OriF1>>, OriF1<<
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1654-plasmid-map-sequence-id-422970

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  6. pLVX-puro-cOVA-IRES-BFP

    Plasmid
    #135074
    Purpose
    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.
    Depositor
    Maria Castro
    Insert
    cOVA-IRES-BFP (OVAL Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    135074-plasmid-map-sequence-id-266791

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  7. pAAV-CAG-tdTomato (codon diversified) (AAV1)

    Viral Prep
    #59462-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid
    #127289
    Purpose
    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)
    Depositor
    Fred Gage
    Article
    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1
    Inserts
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Ngn2 and Ascl1 linked via 2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127289-plasmid-map-sequence-id-308425

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  10. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (AAV5)

    Viral Prep
    #130998-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (#130998). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie
    Promoter
    EF1a
    Tags
    mScarlet
    Available since
    March 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV-syn-dLight1.3b (AAV9)

    Viral Prep
    #135762-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-syn-dLight1.3b (#135762). In addition to the viral particles, you will also receive purified pAAV-syn-dLight1.3b plasmid DNA. Syn-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci
    Promoter
    Syn
    Tags
    None
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  13. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Article
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Use
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    123461-plasmid-map-sequence-id-417805

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  16. pAAV-hSyn-DIO-EGFP (AAV2)

    Viral Prep
    #50457-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-hSyn-DIO-mCherry (AAV Retrograde)

    Viral Prep
    #50459-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  27. pFLAG_WT MDA5

    Plasmid
    #216798
    Purpose
    Expresses wild type (wt) melanoma differentiation-associated protein 5
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 (IFIH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216798-plasmid-map-sequence-id-428831

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  28. pFastBac1 human full-length CENP-E-mNeon-6xHis

    Plasmid
    #180484
    Purpose
    pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells
    Depositor
    Julie Welburn
    Article
    Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1
    Insert
    CENP-E (CENPE Human, human, Synthetic)
    Use
    Tags
    mNeon-6xHis
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    180484-plasmid-map-sequence-id-377471

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  29. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV Retrograde)

    Viral Prep
    #20298-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Oct. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  31. pCMV-T7-EGFP (BPK1098)

    Plasmid
    #133962
    Purpose
    CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription
    Depositor
    Benjamin Kleinstiver
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    133962-plasmid-map-sequence-id-265771

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  34. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE (AAV1)

    Viral Prep
    #51509-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE (#51509). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE plasmid DNA. hSyn-driven, Cre-dependent expression of cytoplasmic tdTomato and presynaptic EGFP (synaptophysin-fused). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do
    Promoter
    phSyn1
    Tags
    tdTomato (cytoplasmic, Cre-dependent), EGFP (presynaptic, C…
    Available since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only

  36. pGL3-NFAT luciferase

    Plasmid
    #17870
    Purpose
    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
    Depositor
    Jerry Crabtree
    Article
    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.
    Insert
    3x NFAT binding sequence (IL2 )
    Use
    Luciferase
    Tags
    IL2 promoter and Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    IL-2 promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    17870-plasmid-map-sequence-id-325133

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  38. pAAV-hSyn-DIO-mCherry (AAV1)

    Viral Prep
    #50459-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV.GFAP.eGFP.WPRE.hGH (AAV5)

    Viral Prep
    #105549-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GFAP.eGFP.WPRE.hGH (#105549). In addition to the viral particles, you will also receive purified pAAV.GFAP.eGFP.WPRE.hGH plasmid DNA. GFAP-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    GFAP
    Tags
    EGFP
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  40. rtTA-N144

    Plasmid
    #66810
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance
    Depositor
    Andrew Yoo
    Article
    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi
    Insert
    Reverse tetracycline-controlled transactivator
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    66810-plasmid-map-sequence-id-418923

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Showing: 921 - 960 of 161255 results