We narrowed to 60,538 results for: Gene
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Plasmid#18925PurposeFLEX switch empty backboneDepositorInsertFLEX switch
UseCre/Lox; SubcloningMutationAn internal Spe1 site is used for subcloning a ge…Available SinceJan. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLOVE-GFP
Plasmid#15949DepositorInsertGreen Fluorescent Protein
UseLentiviralTagsV5ExpressionMammalianMutationThe recombination reaction required to clone &quo…Available SinceNov. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
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BL21(DE3)-R3-pRARE2
Bacterial Strain#26242PurposePhage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.DepositorBacterial ResistanceChloramphenicolAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Bison sgRNA library
Pooled Library#169942PurposeKnockout library targeting ubiquitin ligases, deubiquitinases, and control genes.DepositorUseLentiviralAvailable SinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF)
Pooled Library#1000000113PurposeGenome-wide CRISPR gRNA pooled library for activation of mouse genesDepositorAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter pGL3Basic
Plasmid#32727DepositorAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
CAG-Blimp1
Plasmid#73995PurposePlasmid expressing mouse Blimp1DepositorAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
ONE-GO biosensors
Plasmid Kit#1000000224PurposeOne-vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by GPCRs.DepositorApplicationGene Expression and LabelingVector TypeLentiviral, Mammalian ExpressionAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLOVE-Klf4
Plasmid#15950DepositorInsertKruppel-like factor 4 (Klf4 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationThe recombination reaction required to clone &quo…Available SinceNov. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK1035
Plasmid#8832DepositorInsertTVMV protease
TagsHis and MBP (cleaved)ExpressionBacterialMutationsynthetic gene with codons optimized for E. coliAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-4EBP1_4xAla
Plasmid#38240DepositorInsert4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)
UseLentiviral; Doxycycline inducibleExpressionMammalianMutationT37A, T46A, S65A, T70A (dominant negative)PromoterTet ONAvailable SinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
HisMS2_VLP_RSV
Plasmid#175958PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]DepositorInsertsP gene
M gene
ExpressionBacterialPromoterT7Available SinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-L1PA1-CH-mneo
Plasmid#51288Purposecontains the fully codon optimized ORF1 and ORF2 of the human L1RPDepositorInsertL1 (L1RE1 Human)
TagsmneoI indicator cassette containing an inverted n…ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
L1-neo-TET
Plasmid#51284Purposeexpresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassetteDepositorInsertL1RE1 (L1RE1 Human)
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSV-Rev
Plasmid#12253Purpose3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorInsertHIV-1 GAG/POL
UseLentiviral; PackagingExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-dCas9-VPR
Plasmid#63801PurposepAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectorDepositorInsertdCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionYeastPromoterGPDAvailable SinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9
Plasmid#42230PurposeA human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-mCD8
Plasmid#162747PurposeNFAT-driven ZsGreen-1 reporter gene with mouse CD8 geneDepositorInsert8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
UseRetroviralExpressionMammalianAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AdEasier-1 cells (strain)
Bacterial Strain#16399DepositorBacterial ResistanceAmpicillin and StreptomycinSpeciesEscherichia coliAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralExpressionMammalianPromoterEf1-a and hU6Available SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
BL21∆serB
Bacterial Strain#34929DepositorBacterial ResistanceNoneAvailable SinceFeb. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bARGSer-AxeTxe
Plasmid#192473PurposeSecond-generation bacterial acoustic reporter gene derived from SerratiaDepositorInsertsbARGSer
AxeTxe
ExpressionBacterialMutationthe gene Ser39006_001280 was deleted from the clu…PromoterNative promoter from Enterococcus faecium and pBADAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ251-GERC
Plasmid#47441PurposeExpresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.DepositorInsertGene Expression Reporter Construct
ExpressionBacterialAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
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9X NFAT-apha-MHC-Luc
Plasmid#51941Purpose9 copies of NFAT sites in minimal alphaMHC-pGL-3DepositorInserts9x NFAT binding site
alpha-MHC
UseLuciferaseTagsluciferaseAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only