163,421 results

1. pLV-tetO-myc T58A

   Plasmid

   #19763

   Depositor

   Konrad Hochedlinger

   Article

   Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

   Insert

   c-myc (MYC Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T58A

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2-39

   Plasmid

   #118086

   Purpose

   ZB-linker-split C-terminal T7 RNAP expression plasmid

   Depositor

   Bryan Dickinson

   Article

   Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

   Insert

   ZB-linker-split C-terminal T7 RNAP expression plasmid
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFR_CrPV_xb

   Plasmid

   #11509

   Depositor

   Phil Sharp

   Article

   Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

   Insert

   Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pl-Synapsin-YFP-NaVII-III

   Plasmid

   #91246

   Purpose

   Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)

   Depositor

   Matthew Grubb

   Article

   Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.

   Insert

   Nav1.2 (Scn2a Rat)
   

   Use

   Lentiviral

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   The insert codes only for the intracellular loop …

   Promoter

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-ER beta

   Plasmid

   #28237

   Depositor

   Michael Mancini

   Insert

   ER beta (ESR2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1A-GFP-N2 (FR1)

   Plasmid

   #19364

   Purpose

   Gateway entry vector for C-term eGFP fusion, frame N2

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT/FLAG/HA-DEST PUM2

   Plasmid

   #40292

   Depositor

   Thomas Tuschl

   Article

   Hafner et al Cell. 2010 Apr 2;141(1):129-41.

   Insert

   Pumilio homolog 2 (PUM2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. sfGFP-pBAD

   Plasmid

   #54519

   Purpose

   Localization: Protein Expression Vector , Excitation: 485, Emission: 507

   Depositor

   Michael Davidson, Geoffrey Waldo

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Use

   Tags

   6xHis and SfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3-EGFP-Cdc42(wt)

   Plasmid

   #12599

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (WT) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. V5-LOV-Turbo-NES

    Plasmid

    #199545

    Purpose

    expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NES and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFast-hHus1

    Plasmid

    #222469

    Purpose

    Expresses hHus1 in insect cells

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

    Insert

    hHus1 (HUS1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. β3-integrin-YFP

    Plasmid

    #26653

    Depositor

    Jonathan Jones

    Article

    Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

    Insert

    β3-integrin (ITGB3 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIZ-Flag6His-Siwi

    Plasmid

    #50560

    Purpose

    Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

    Depositor

    Yukihide Tomari

    Article

    Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

    Insert

    Siwi (Aubergine )
    

    Use

    Tags

    6xHIS and FLAG

    Expression

    Insect

    Mutation

    Promoter

    OpIE2

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDisplay-mSA-EGFP-TM

    Plasmid

    #39863

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin, EGFP, TM helix
    

    Use

    Tags

    EGFP, FLAG, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CamK2a(0.4)-Opto-cytTrkB(E281A)-HA

    Plasmid

    #180589

    Purpose

    Opto-cytTrkB

    Depositor

    Won Do Heo

    Article

    Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

    Insert

    TrkB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    PHR(E281A)

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. mtsCOXVIIIx2-B-gTEMP/pcDNA3

    Plasmid

    #199806

    Purpose

    Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Use

    Tags

    mtsCOXVIIIx2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. OMM-V5-LOV-Turbo

    Plasmid

    #199665

    Purpose

    expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    AKAP1 transmembrane domain and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSG06-pLac-NRI

    Plasmid

    #102463

    Purpose

    Expresses NRI protein in E. coli. The expression is controlled by a pLac promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 122606

    Insert

    NRI (ntrC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6-CENP-A-mCherry

    Plasmid

    #89767

    Purpose

    Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    CENP-A (CENPA Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF1243

    Plasmid

    #143776

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1-TRKB-V5/HIS

    Plasmid

    #205614

    Purpose

    Expression of human TRKB receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TRKB receptor tyrosine kinase (NTRK2 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLENTI_hACE2_PURO

    Plasmid

    #155295

    Purpose

    Lentiviral vector to generate hACE2 stable expressing cell line

    Depositor

    Raffaele De Francesco

    Insert

    hACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    C9

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pCAG-EGFP(HiBiT)

    Plasmid

    #162589

    Purpose

    N-terminally HiBiT-tagged EGFP under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    EGFP
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. BL21(DE3) ΔserC

    Bacterial Strain

    #197656

    Purpose

    Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Bacterial Resistance

    None

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

26. Vimentin-pLJM5

    Plasmid

    #189868

    Purpose

    Expresses human Vimentin in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

    Insert

    Vimentin
    

    Use

    Tags

    m-Cherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV7 mitoDendra2

    Plasmid

    #157757

    Purpose

    Expression of the fluorescent protein Dendra2 targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    Dendra2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MTS coxVIII +Dendra2

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

    Plasmid

    #183951

    Purpose

    Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYL322d1/Vn-C

    Plasmid

    #183154

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBITE-EGFRvIII-F263-OKT3

    Plasmid

    #190680

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRBC-sfGFP-150TAG

    Plasmid

    #174076

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTRIPZ (M)-HT-Cbx2

    Plasmid

    #82510

    Purpose

    Expresses Halotag-Cbx2 fusion proteins in mammalian cells

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 2 (CBX2 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV-hSyn-Ribo-jGCaMP8s (AAV1)

    Viral Prep

    #167572-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn-Ribo-jGCaMP8s (#167572). In addition to the viral particles, you will also receive purified AAV-hSyn-Ribo-jGCaMP8s plasmid DNA. Synapsin-driven neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Promoter

    Syn

    Tags

    None

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

34. TFORF1513

    Plasmid

    #143513

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX3-1 (NKX3-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-OTp-GCaMP6s

    Plasmid

    #192945

    Purpose

    To drive GCaMP6s under the control of mouse oxytocin promoter

    Depositor

    Kazunari Miyamichi

    Article

    Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. W118-1_hTGM1-Flag

    Plasmid

    #180406

    Purpose

    lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM1 (TGM1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRB897

    Plasmid

    #124587

    Purpose

    C-terminal fusions of C. albicans optimized mScarlet fluorescent protein.

    Depositor

    Richard Bennett

    Article

    Frazer et al Curr Protoc Microbiol. 2019 Feb 12:e76.

    Type

    Empty backbone

    Use

    Synthetic Biology; Candida albicans expression

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTOX1

    Plasmid

    #127446

    Purpose

    Encodes YhaV toxin. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    yhaV toxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. anti-Desmin_D7-pBIOCAM5

    Plasmid

    #39346

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Insert

    scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)
    

    Use

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge