Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

173,387 results

Showing: 11601 - 11640 of 173387 results

  3. pcDNA5-FRT-TOgw-N-mCherry

    Plasmid
    #223948
    Purpose
    Gateway cloning of genes of interest with an N-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223948-plasmid-map-sequence-id-450831

    Enlarge

  4. TFORF0404

    Plasmid
    #141606
    Purpose
    Lentiviral vector for overexpressing the CTCFL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CTCFL (CTCFL Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141606-plasmid-map-sequence-id-409169

    Enlarge

  10. pAAV-Ef1a-Con/Foff 2.0-sRGECO (AAV8)

    Viral Prep
    #137127-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-sRGECO (#137127). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-sRGECO plasmid DNA. EF1a-driven, Cre-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available Since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  13. TFORF2048

    Plasmid
    #143332
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRDM9 (PRDM9 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143332-plasmid-map-sequence-id-408544

    Enlarge

  14. pAAV-CaMKII-ChrimsonR-tdTomato

    Plasmid
    #99231
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    CaMKII
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99231-plasmid-map-sequence-id-219823

    Enlarge

  17. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid
    #127370
    Purpose
    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127370-plasmid-map-sequence-id-246351

    Enlarge

  20. pRXG

    Plasmid
    #113643
    Purpose
    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113643-plasmid-map-sequence-id-217116

    Enlarge

  23. pBad-CA TAG186

    Plasmid
    #105837
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 186
    Promoter
    pBad/arabinose
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105837-plasmid-map-sequence-id-205518

    Enlarge

  30. sAB-K29

    Plasmid
    #204735
    Purpose
    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
    Depositor
    Minglei Zhao
    Article
    Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d
    Insert
    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
    Available Since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204735-plasmid-map-sequence-id-412652

    Enlarge

  34. pX330 Mouse 5' Npm1 gRNA

    Plasmid
    #127900
    Purpose
    WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene
    Depositor
    Mark Groudine
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127900-plasmid-map-sequence-id-249686

    Enlarge

  39. T7-VEE-OKS-iG

    Plasmid
    #58974
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58974-plasmid-map-sequence-id-240741

    Enlarge

Showing: 11601 - 11640 of 173387 results