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HyGCaMP6s
Plasmid#102558PurposeExpression of GCaMP6s in HydraDepositorInsertGCaMP6s
UseHydra expressionTagsActin/GCaMP6s fusion protein (C terminal on backb…ExpressionMutationPromoterAvailable sinceOct. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sequestosome1-N-18
Plasmid#55132PurposeLocalization: Membrane Scaffold, Excitation: 587, Emission: 610DepositorInsertSequestosome1 (SQSTM1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Beta-arrestin2 GFP WT
Plasmid#35411DepositorInsertβarrestin2 (Arrb2 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-BirA-V5-neo
Plasmid#100548PurposeExpresses C-terminal V5-tagged BirA biotin ligaseDepositorInsertC-terminal V5-tagged BirA
UseTagsV5, HisExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pSBbi-RB
Plasmid#60522PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJL 89
Plasmid#98558PurposeUseful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterEnh 35SAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183523PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationI146M/G174SPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0490
Plasmid#143030PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZSCAN4 (ZSCAN4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Bsd
Plasmid#214370PurposeCloning vector with BlasticidinDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIH-TFAM-GFP
Plasmid#113704PurposeFluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selectionDepositorInsertTFAM (Tfam Mouse)
UseRetroviralTagseGFPExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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hCRISPRa-v2, Membrane Proteins (h6), top 5 sgRNAs/gene
Pooled Library#83985PurposeHuman CRISPRa Pooled Library targeting membrane proteinsDepositorAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG HRP-TM
Plasmid#44441DepositorArticleInsertHorseradish peroxidase fused to the transmembrane domain of PDGFR
UseTagsHA tag and myc tagExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-Nono-sh1
Plasmid#127650PurposeKnock-down of human NONODepositorInsertNONO shRNA (NONO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcs2-GFP-IRES-mCherry
Plasmid#226098PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD1
Plasmid#20183DepositorInsertNotch 1 Intracellular Domain (Notch1 Mouse)
UseTags3XFLAGExpressionMammalianMutationContains murine Notch 1 Intracellular Domain (Val…PromoterCMVAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK-Myc-Smurf2
Plasmid#13678DepositorInsertSmurf2 (SMURF2 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-CrN173
Plasmid#22016DepositorInsertCrN173
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET30a_HIV-RT
Plasmid#153311PurposeBacterial expression of HIV reverse transcriptaseDepositorInsertHIV Reverse Transcriptase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn1-mTurquoise2
Plasmid#99125PurposeAn AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoterDepositorInsertmTurquoise2
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVS1
Plasmid#33143DepositorInsertLacO (256 copies)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-eGFP-WPRE
Plasmid#218743PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET12a-PAPI
Plasmid#19961DepositorInsertAnnexin V (ANXA5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-GFP-wt-lamin A
Plasmid#17662DepositorInsertGFP-lamin A (LMNA Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMLH1
Plasmid#129426PurposeExpression human MLH1 protein using baculovirusDepositorInserthuman MLH1 (MLH1 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hGPR56-IRES-GFP
Plasmid#52297PurposeExpresses human GPR56 and GFP in mammalian cells.DepositorInsertHuman GPR56 coding sequence (ADGRG1 Human)
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP712
Plasmid#65768PurposeBacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNADepositorInsertmammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
UseCRISPRTags3x FLAG and NLSExpressionBacterialMutationD10A and H840A mutations in Cas9PromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSynapsin-PE2-Cterminal-lenti
Plasmid#135956PurposeNeuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) C-terminal
UseLentiviralTagsExpressionMammalianMutationMMLV RT(D200N, T306K, W313F, T330P, L603W)PromoterhSynapsinAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGK-AID-NTERM-GFP
Plasmid#185773PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Green Glifon4000
Plasmid#126208Purposegreen fluorescent glucose sensor for mammalian cellsDepositorInsertGreen Glifon4000
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-JMJD2B
Plasmid#24181PurposeMammalian expression of human JMJD2B with HA tagDepositorInsertJMJD2B (KDM4B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-DYRK3
Plasmid#23635DepositorInsertDYRK3 (DYRK3 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ptet-RFP-shR-rtTA
Plasmid#35625PurposeTet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).DepositorTypeEmpty backboneUseAAV and RNAi; Tet inducibleTagsExpressionMammalianMutationPromoterPtetAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) (AAV PHP.eB)
Viral Prep#214853-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) (#214853). In addition to the viral particles, you will also receive purified AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) plasmid DNA. minBG-driven expression of CoChR-EGFP striatal indirect pathway medium spiny neurons (D2 MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsEGFPAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSin-EF2-Oct4-Pur
Plasmid#16579DepositorInsertHomo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
Inzolia library in pRDA_052
Pooled Library#209551PurposeThe human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.DepositorUseCRISPR and LentiviralAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H2B-iRFP720
Plasmid#128961PurposeLentiviral plasmid for expression of far red iRFP720 nuclear markerDepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterhPGKAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only
