161,273 results

1. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

   Plasmid

   #161926

   Purpose

   Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   rTetR(SE-G72P)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB1C3-Lux

   Plasmid

   #109383

   Purpose

   Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pARABAD-LUX operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1a-H2B-miRFP670

   Plasmid

   #212668

   Purpose

   Express tagged H2B

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   miRFP670

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-EGFP-Cdc42-wt

   Plasmid

   #12975

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLminP_Luc2P_RE59

   Plasmid

   #90404

   Purpose

   STAT3 - Sis-inducible element (SIE) gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   IRF1

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tet-pLKO-FOXM1-shRNA-81

   Plasmid

   #89497

   Purpose

   Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

   Depositor

   Adam Karpf

   Insert

   FOXM1 (FOXM1 Human)
   

   Use

   Lentiviral and RNAi; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB_TRE3G_scFV-sfGFP-Ezh2FL

   Plasmid

   #235575

   Purpose

   Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiPGK Hygro DEST ERKKTRmCerulean3

   Plasmid

   #90229

   Purpose

   Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT-Bip-GFP:V5:KDEL

   Plasmid

   #69917

   Purpose

   An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

   Depositor

   Kenneth Irvine

   Article

   Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

   Insert

   GFP
   

   Use

   Tags

   Drosophila BiP signal sequence, KDEL ER targeting…

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Anti-Strep [IPI-C23.21] (Recombinant Antibody)

    Antibody

    #218105-rAb

    Purpose

    Anti-Strep tag chimeric recombinant antibody, with fused mouse variable and rabbit constant domains; binds to WSHPQFEK sequence.

    Depositor

    Institute for Protein Innovation (IPI)

    Article

    Institute for Protein et al Unpublished

    Recommended Applications

    Western Blot

    Reactivity

    Source Species

    Rabbit

    Isotype

    IgG1

    Trial Size

    Not available to purchase

    Available since

    May 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

11. PB_tre_Cas9

    Plasmid

    #126029

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible Cas9

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    humanized S. pyrogenes Cas9
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1_sgRNA

    Plasmid

    #100554

    Purpose

    Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    AAVS1 sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSH124-ssr

    Plasmid

    #198025

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Ap/Cb resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ApR promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. VP16-ER alpha

    Plasmid

    #11351

    Depositor

    Donald McDonnell

    Article

    Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

    Insert

    ER alpha (ESR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZsGreen1-N1

    Plasmid

    #54702

    Purpose

    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    ZsGreen1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. DRD2-DuET

    Plasmid

    #213228

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    DRD2 (DRD2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8)

    Viral Prep

    #105545-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225699

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K36M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Mutation

    K36M

    Promoter

    pEF1A

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-PERK(LD)-EGFP

    Plasmid

    #225680

    Purpose

    Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

    Depositor

    Likun Wang

    Article

    Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAC-LYCipi

    Plasmid

    #53279

    Purpose

    Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTP1122

    Plasmid

    #104159

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLENTI6.3/AR-GC-E2325

    Plasmid

    #85128

    Purpose

    exogenous AR expression

    Depositor

    Karl-Henning Kalland

    Article

    Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

    Insert

    androgen receptor (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV5-ActRIIA

    Plasmid

    #11754

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    Activin receptor IIA (Acvr2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. YIplac211-SEC7-mCherry2Bx6

    Plasmid

    #105267

    Purpose

    Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Sec7 portion (SEC7 Budding Yeast)
    

    Use

    Tags

    mCherry2Bx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGEX SH3(2)-4R

    Plasmid

    #112090

    Purpose

    Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-4R (NCK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDB5322

    Plasmid

    #204832

    Purpose

    An integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW4 promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pwtCas9-bacteria

    Plasmid

    #44250

    Purpose

    aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    wild-type Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP-h53BP1 (siRNA resistant)

    Plasmid

    #110301

    Purpose

    Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    GAACGAGGA to GAGCGGGGC

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCDH-p18-CIBN

    Plasmid

    #188990

    Purpose

    A lentiviral plasmid encoding lysosome-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Use

    Tags

    p18

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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