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161,377 results

Showing: 1241 - 1280 of 161377 results

  2. f-CTS-EGFP

    Plasmid
    #86064
    Purpose
    A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains ciliary targeting signal (3870-3887 amin…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86064-plasmid-map-sequence-id-179754

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  3. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid
    #115161
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.
    Inserts
    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    Use
    AAV
    Tags
    hM3Dq-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115161-plasmid-map-sequence-id-220939

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  4. AAV-EF1a-jGCaMP8m-WPRE (AAV Retrograde)

    Viral Prep
    #176757-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV-EF1a-jGCaMP8m-WPRE (#176757). In addition to the viral particles, you will also receive purified AAV-EF1a-jGCaMP8m-WPRE plasmid DNA. EF1a-driven expression of calcium sensor GCaMP8m. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    EF1a
    Tags
    None
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  10. pDONR221-SLC25A4_STOP

    Plasmid
    #161148
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC25A4 (SLC25A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161148-plasmid-map-sequence-id-398135

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  11. pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

    Plasmid
    #175549
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.
    Depositor
    Elzo de Wit
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    WAPL (Wapl Mouse)
    Use
    Mouse Targeting
    Tags
    AID[71-114]-eGFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175549-plasmid-map-sequence-id-345088

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  13. mPA-GFP-C1

    Plasmid
    #54522
    Purpose
    Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54522-plasmid-map-sequence-id-393320

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  19. pAAV2-CAG-mGreenLantern-KASH

    Plasmid
    #191094
    Purpose
    To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mGreenLantern-KASH
    Use
    AAV and Synthetic Biology
    Tags
    mGreenLantern fused to the KASH domain of Nesprin…
    Expression
    Mammalian
    Mutation
    Optimizzation was done using the Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191094-plasmid-map-sequence-id-380125

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  20. BRD7 (BRD7A-c005)

    Plasmid
    #98245
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD7A (BRD7 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00439:Bromodomain 136-223
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98245-plasmid-map-sequence-id-193423

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  21. SiC-V1-Scr

    Plasmid
    #133042
    Purpose
    SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.
    Depositor
    Sriram Neelamegham
    Article
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    133042-plasmid-map-sequence-id-264423

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  32. pD649-HAsp-AMIGO3-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156805
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    AMIGO3 (AMIGO3 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156805-plasmid-map-sequence-id-420997

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  33. EFNB1-bio-His

    Plasmid
    #51749
    Purpose
    Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    EFNB1 (EFNB1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51749-plasmid-map-sequence-id-79334

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  36. Aiolos AA130-189 in Artichoke

    Plasmid
    #74451
    Purpose
    Monitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    SFFV and EF1a
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74451-plasmid-map-sequence-id-140674

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  39. P2RX1-bio-His

    Plasmid
    #53359
    Purpose
    Expresses full-length P2X purinoceptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    P2RX1 (P2RX1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53359-plasmid-map-sequence-id-83082

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Showing: 1241 - 1280 of 161377 results