-
Plasmid#122147PurposeSelf-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.DepositorInsertFTH1 (FTH1 Human)
UseLentiviralTagsNLS-iLID-GFPExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV2/9 REP-AAP-ΔCAP
Plasmid#205991PurposeRep and AAP protein expression for AAV CREATE librariesDepositorInsertAAV9 Rep and AAP proteins for CREATE libraries
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-off TauRD (P301L/V337M)
Plasmid#188572PurposeDox-repressible expression of the Tau repeat domainDepositorInsertTau repeat domain (MAPT Human)
UseLentiviralTagsMycExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS1099: Dual-sgRNA.Design 4
Plasmid#159537PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 with two guide RNA cassettes
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianMutationPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEJS1089: mini-AAV.sgRNA.Nme2Cas9
Plasmid#159536PurposeDelivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 KbDepositorInsertNme2Cas9 with single guide RNA cassette
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianMutationPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEJS1096 Dual-sgRNA.Design 1
Plasmid#159538PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 nuclease with two guide RNA cassettes with promoters
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianMutationPromoterU1aAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau AP
Plasmid#46905Purposeexpresses EGFP tagged Tau AP in mammalian cellsDepositorInsertTau AP (MAPT Human)
UseTagsEGFPExpressionMammalianMutationall 14 S/P or T/P amino acid residues (T111, T153…PromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-catΔ
Plasmid#222501PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; C706A and R832…PromoterEFSAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-ICAM1
Plasmid#156462PurposeExpresses a rabbit Fc-ICAM1 fusion protein in mammalian cellsDepositorInsertIntercellular Adhesion Molecule 1 (Icam1 Mouse)
UseTagsICAM1-rFcExpressionBacterial and MammalianMutationendogenous signal peptide replaced with IL2 signa…PromoterhEF1-HTLCAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1.sh.beta-catenin.1248
Plasmid#19761DepositorInsertsmall hairpin RNA against beta-catenin (CTNNB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1.sh.beta-catenin.2279
Plasmid#19762DepositorInsertsmall hairpin RNA against beta-catenin (CTNNB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-SpdCas9-Dnmt3A/3L-WT
Plasmid#222502PurposeExpresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherryDepositorInsertsS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
mCherry
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterTRE3GV and hPGKAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC403 LacZ
Plasmid#111184PurposeControl inducible vector with LacZ (V5 tagged)DepositorInsertlacZ
UseLentiviralTagsV5ExpressionMutationPromoterTREAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-KKKAAA
Plasmid#171219PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.DepositorInsertAR-KKKAAA (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-K630A-K632A-K633APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC401 LacZ
Plasmid#111183PurposeControl inducible vector with LacZ (V5 tagged)DepositorInsertlacZ
UseLentiviralTagsV5ExpressionMutationPromoterTREAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 Luciferase
Plasmid#117072Purposesingle guide RNA targeting LuciferaseDepositorInsertLuciferase
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pABD1031
Plasmid#68004PurposeProtein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in plantaDepositorInsertPHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
UseTagseYFPExpressionPlantMutationPromoter2X Cauliflower Mosaic Virus 35S promoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL delta K5S
Plasmid#34594DepositorInsertKLF4 (KLF4 Human)
UseTagsHA tagExpressionMammalianMutationdeletion of K5S, corresponding to bases 1781 to 2…PromoterCMVAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pABD1275
Plasmid#68017PurposePIF1 protein overproduction for purification from bacterial cellsDepositorInsertPHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
UseTags6x-HisExpressionBacterialMutationCodon Optimized for bacterial expression (GenScri…PromoterT7Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBpuro - HA KLF4 FL delta K5S
Plasmid#34592DepositorInsertKLF4 (KLF4 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationdeletion of K5S, corresponding to bases 1781 to 2…PromoterLTRAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
STIM1-RspA-NFAST
Plasmid#233606PurposeExpression of hSTIM1-RspA-NFASTDepositorInsertSTIM1 (STIM1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-V1
Plasmid#222498PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; R887E in Dnmt3APromoterEFSAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-V2
Plasmid#222499PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E814G in Dnmt3APromoterEFSAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-V3
Plasmid#222500PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; R887E and E814…PromoterEFSAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-SpdCas9-Dnmt3A/3L-V1
Plasmid#222503PurposeExpresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherryDepositorInsertsS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
mCherry
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; R887E in Dnmt3APromoterTRE3GV and hPGKAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-SpdCas9-Dnmt3A/3L-V2
Plasmid#222505PurposeExpresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherryDepositorInsertsS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
mCherry
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E814G in Dnmt3APromoterTRE3GV and hPGKAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-SpdCas9-Dnmt3A/3L-V3
Plasmid#222506PurposeExpresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherryDepositorInsertsS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
mCherry
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; R887E and E814…PromoterTRE3GV and hPGKAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-SpdCas9-Dnmt3A/3L-catΔ
Plasmid#222508PurposeExpresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherryDepositorInsertsS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
mCherry
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; C706A and R832…PromoterTRE3GV and hPGKAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A
Plasmid#213527PurposeExpresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92ADepositorInsertNSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationD90A and E92APromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_hRNMT_RAM
Plasmid#213528PurposeExpresses the human RNMT-RAM complexDepositorInsertsUseTagsHis6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14
Plasmid#213518PurposeExpresses NSP14 of SARS-CoV-2DepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
UseTagsN-terminal His6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_OC43_NSP14
Plasmid#213519PurposeExpresses NSP14 of hCoV OC43DepositorInserthCoV OC43 NSP14 (ORF1ab hCoV OC43)
UseTagsHis-SUMO tagExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_NL63_NSP14
Plasmid#213520PurposeExpresses NSP14 of hCoV NL63DepositorInserthCoV NL63 NSP14 (ORF1ab hCoV NL63)
UseTagsHis6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_229E_NSP14
Plasmid#213521PurposeExpresses NSP14 of hCoV 229EDepositorInserthCoV 229E NSP14
UseTagsHis6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_V290A
Plasmid#213522PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation V290ADepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationchanged V290 to APromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_N306K
Plasmid#213523PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation N306KDepositorInsertSARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationchanged N306 to KPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_P335S
Plasmid#213524PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation P335SDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationchanged P335 to SPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_P335H
Plasmid#213525PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation P335HDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationchanged P335 to HPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_H424R
Plasmid#213526PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation H424RDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationchanged H424 to RPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HEK4-53bp-tag-insertion-pegRNA
Plasmid#227673PurposePegRNA for 53 bp insertionDepositorInsertSpCas9 sgRNA scaffold (EPHA3 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only