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Plasmid#193309PurposeCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
UseCRISPRTagsExpressionMammalianMutationnot applicablePromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRGE31
Plasmid#50929PurposeExpressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRice snoRNA U3 and dual 35S promoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-GMR-eya(shRNA)
Plasmid#157991PurposepBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV1200
Plasmid#111429PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-TRE3G-TdTomato
Plasmid#192658PurposeA plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomatoDepositorInsertTdTomato
UseTagsExpressionMammalianMutationPromoterU6, TRE3GAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH102
Plasmid#128207PurposesgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertsgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_0
Plasmid#127553PurposeDrosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 0
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_1
Plasmid#127554PurposeDrosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 1
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK008
Plasmid#128225Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 2DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 2
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK009
Plasmid#128226Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 3DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 3
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LBJJ382
Plasmid#117518PurposeAlso known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAK005
Plasmid#128218Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 5DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 5
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK003
Plasmid#128216Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 3DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 3
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK004
Plasmid#128217Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 4DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 4
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK006
Plasmid#128219Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 6DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 6
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK002
Plasmid#128215Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 2DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 2
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459_gRNA pAS single_hspCas9
Plasmid#193311PurposeCas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
UseCRISPRTagsExpressionMammalianMutationnot applicablePromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-J23109
Plasmid#113150PurposePlasmid constitutively expressing Cas9 proteinDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-guideless
Plasmid#129464PurposeDerived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.DepositorInsertGuideless gRNA backbone
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9-HF1
Plasmid#104173PurposeCas9-HF1 mammalian expressionDepositorInsertCas9-HF1
UseCRISPRTagsHAExpressionMammalianMutationN497A, R661A, Q695A, Q926APromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3s-eCas9-1.1
Plasmid#104172PurposeeCas9-1.1 mammalian expressionDepositorInserteCas9-1.1
UseCRISPRTagsHAExpressionMammalianMutationK848A, K1003A, R1060APromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:Cas9green; T3_gRNA
Plasmid#199335PurposepDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNADepositorInsertszCREST3
Cas9
nrg1 type III sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-Aa-artsg13 (BsaI)
Plasmid#188279PurposeMammalian expression, Genome editingDepositorInsertAa-artsg13
UseCRISPRTagsExpressionMammalianMutationnonePromoterU6Available sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM100
Plasmid#127225PurposeAtU6::sgRNA targeting PDSDepositorInsertsgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP2367
Plasmid#160076PurposeZ. mobilis CRISPRi, promoter C, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-miniCMV-TdTomato
Plasmid#192656PurposeA plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomatoDepositorInsertTdTomato
UseTagsExpressionMammalianMutationPromoterU6, miniCMVAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH85
Plasmid#128198Purposeclassic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)DepositorInsertclassic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY108
Plasmid#130938PurposeEngineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH86
Plasmid#128199Purposeimproved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)DepositorInsertimproved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY134
Plasmid#130962PurposeEngineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH88
Plasmid#128201PurposesgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)DepositorInsertsgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0058
Plasmid#117539PurposeLevel1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (SaCas9)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLY103
Plasmid#130937PurposeEngineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH99
Plasmid#128204Purposeclassic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertclassic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH100
Plasmid#128205Purposeimproved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertimproved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH101
Plasmid#128206PurposesgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertsgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gATRIP_A
Plasmid#211537PurposesgRNA-A against ATRIPDepositorInsertsgRNA ATRIP
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gRAD9A_B
Plasmid#211612PurposesgRNA-B against RAD9DepositorInsertsgRNA RAD9A
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gRHNO1_B
Plasmid#211614PurposesgRNA-B against RhnoDepositorInsertsgRNA RHNO1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gRHNO1_A
Plasmid#211613PurposesgRNA-A against RhnoDepositorInsertsgRNA RHNO1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only