We narrowed to 7,192 results for: GCA

1. Lenti-eCas9-sgNT

   Plasmid

   #140238

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330-UGCG-KO

   Plasmid

   #80010

   Purpose

   gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   UGCG (UGCG Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pU6-SAG1-DHFR

   Plasmid

   #80322

   Purpose

   Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Insert

   SAG1 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR-TER+-shLKB1-Hu

   Plasmid

   #61243

   Purpose

   Entry clone encoding shRNA to human LKB1

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   LKB1 (STK11 Human)
   

   Use

   Entr clone

   Tags

   Expression

   Mutation

   Promoter

   H1/TO

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

5. sg14x(MS2) MUC4.1

   Plasmid

   #101153

   Purpose

   gRNA with 14 MCP binding site

   Depositor

   Mazhar Adli

   Article

   Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

   Insert

   MUC4 (MUC4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. shLacZ

   Plasmid

   #42559

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shLacZ
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pLKO.1-sh-mSlug-4

   Plasmid

   #40648

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-mSlug-4 (Snai2 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pTC394

   Plasmid

   #91224

   Purpose

   protoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNA targeting tomato ANT1
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti CRISPR V2 sgMTHFD1L_1

   Plasmid

   #106316

   Purpose

   Express Cas9 and sgRNA targeting MTHFD1L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD1L
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO-STAG2 shRNA 1221

    Plasmid

    #31978

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pSUPER IKKbeta

    Plasmid

    #26209

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    pSUPER IKK beta (IKBKB Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pTorPE-G-GECO1.2

    Plasmid

    #32468

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.2
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSUPERIORpuro-shGAS5

    Plasmid

    #46370

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

    Insert

    GAS5 (GAS5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pLKO-puro-sh-mFAK B

    Plasmid

    #37018

    Depositor

    Bob Weinberg

    Article

    Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

    Insert

    sh-mFAK B (Ptk2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 31, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pSMP-G9A_4

    Plasmid

    #36334

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

16. px330-UFL1 sgRNA2

    Plasmid

    #134636

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA2 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTX209

    Plasmid

    #89269

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01 and OsPDS-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pDECKO_TFRC_B

    Plasmid

    #72626

    Purpose

    Expresses two gRNAs targeting the TFRC promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward TFRC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. psiCHECK2-miR-34 MT

    Plasmid

    #78259

    Purpose

    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    mutated miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Tags

    Expression

    Mammalian

    Mutation

    nucleotides 2-5 and 10-11 changed from CCGT to GG…

    Promoter

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT2SCb

    Plasmid

    #59385

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat2
    

    Use

    Template

    Tags

    Expression

    Mutation

    The 3’-end of the chloramphenicol resistance gene…

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GG-EBNA-EEA-guide1-PGK-Puro

    Plasmid

    #102904

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TET-pLKO.1 PURO shSMAD4 #2

    Plasmid

    #83091

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human SMAD4

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shSMAD4 (SMAD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

25. pLmoCascade-IL1RNcrRNA

    Plasmid

    #126487

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo IL1RN cr03
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDECKO_Malat1_B

    Plasmid

    #72621

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-TO-HA-SH3BP1 (RNAi-res)

    Plasmid

    #136645

    Purpose

    Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1 (RNAi resistant) (SH3BP1 Human)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Oct4-Cr1

    Plasmid

    #66989

    Purpose

    Expresses gRNA that targets stop codon for OCT4

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    gRNA for Crispr targeting the OCT4 stop codon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

29. plko.1/shMCT4 477

    Plasmid

    #99598

    Purpose

    Knockdown of MCT4 expression

    Depositor

    Eli Bar

    Article

    Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

    Insert

    MCT4 (SLC16A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTorPE-B-GECO1

    Plasmid

    #32469

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    B-GECO1.0
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

    Plasmid

    #136646

    Purpose

    Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LentiCRISPR-sgUFSP2

    Plasmid

    #86134

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting UFSP2

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA targeting UFSP2 (UFSP2 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. gh53

    Plasmid

    #106726

    Purpose

    expression of gRNA targeting ST3GAL5

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST3GAL5 (ST3GAL5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO.1P shABCB7_2

    Plasmid

    #102969

    Purpose

    Suppression of ABCB7

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shABCB7_2 (ABCB7 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLKO.1P shABCB7_1

    Plasmid

    #102968

    Purpose

    Suppression of ABCB7

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shABCB7_1 (ABCB7 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

    Plasmid

    #114729

    Purpose

    3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M5, sgRNA M7, sgRNA M9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti CRISPR sgSHMT2_1

    Plasmid

    #106308

    Purpose

    Express Cas9 and sgRNA targeting SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDECKO_Malat1_D

    Plasmid

    #72623

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. px330-MRPsg2

    Plasmid

    #87190

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 2

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #2 (RMRP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PX458_CIC_iso1_2

    Plasmid

    #135744

    Purpose

    Encodes gRNA for 3' target of human CIC_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    CIC_iso1 gRNA (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge