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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 23,544 results for: CHI

Showing: 4351 - 4400 of 23544 results

  1. pGBW-m4046773

    Plasmid
    #145708
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145708-plasmid-map-sequence-id-298372

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  2. pGBW-m4046778

    Plasmid
    #145618
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145618-plasmid-map-sequence-id-298351

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  3. pGBW-m4046904

    Plasmid
    #145591
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145591-plasmid-map-sequence-id-298327

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  6. pHL 1454

    Plasmid
    #53019
    Purpose
    AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    fimB
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLlac0-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pACCS3-1

    Plasmid
    #78559
    Purpose
    4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.
    Depositor
    James Kaper
    Article
    Giron et al Vaccine. 1995 Jul;13(10):939-46.
    Insert
    cs3 operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tet
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pHL 1916

    Plasmid
    #53023
    Purpose
    AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    fimB
    Use
    Synthetic Biology
    Tags
    Asp terminator and RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222016
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222016-plasmid-map-sequence-id-457428

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  24. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid
    #221844
    Purpose
    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Inserts
    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    GACG and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221844-plasmid-map-sequence-id-437475

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  28. pGWT35S-VC155

    Plasmid
    #194051
    Purpose
    Transient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)
    Depositor
    Yutaka Kodama
    Article
    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-
    Insert
    VC155 (mVenus β-strands 8 to 11, amino acids 155−239)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    194051-plasmid-map-sequence-id-382266

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  30. pGWT35S-VN154

    Plasmid
    #194050
    Purpose
    Transient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)
    Depositor
    Yutaka Kodama
    Article
    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-
    Insert
    VN154 (mVenus β-strands 1 to 7, amino acids 1−154)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S promoter
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    194050-plasmid-map-sequence-id-382268

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Showing: 4351 - 4400 of 23544 results