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20,401 results

Showing: 4651 - 4700 of 20401 results

  3. pCAG-NLS-HA-Dre

    Plasmid
    #51272
    Purpose
    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    Dre
    Use
    Tags
    NLS-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51272-plasmid-map-sequence-id-334322

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  4. pJR103

    Plasmid
    #187242
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187242-plasmid-map-sequence-id-374822

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  6. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid
    #71390
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.
    Depositor
    Thomas Cech
    Article
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71390-plasmid-map-sequence-id-128451

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  7. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh and U6
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    91854-plasmid-map-sequence-id-176529

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  8. ADRB1-Tango

    Plasmid
    #66219
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRB1 (ADRB1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66219-plasmid-map-sequence-id-456477

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  11. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Expression
    Mutation
    Promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138148-plasmid-map-sequence-id-318593

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  12. pAAV-Ef1a-Coff/Fon-mCherry (AAV8)

    Viral Prep
    #137134-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-mCherry (#137134). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-mCherry plasmid DNA. EF1a-driven, Flp-dependent expression of mCherry (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only

  17. pBV-Luc

    Plasmid
    #16539
    Purpose
    luciferase reporter plasmid with very low basal activity
    Depositor
    Bert Vogelstein
    Article
    He et al Cell. 1999 Oct 29. 99(3):335-45.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
    16539-plasmid-map-sequence-id-233265

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  19. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (AAV2)

    Viral Prep
    #50467-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine (#50467). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine plasmid DNA. CaMKIIa-driven HA-tagged hM4D(Gi) and bicistronic mCitrine expression, for chemogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCitrine
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only

  21. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid
    #127366
    Purpose
    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127366-plasmid-map-sequence-id-246342

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  24. 14UAS PSD95:GFP 5UAS DSRedExpress

    Plasmid
    #74315
    Purpose
    Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish
    Depositor
    Martin Meyer, Stephen Smith
    Article
    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub
    Inserts
    zebrafish PSD95
    DsRedExpress
    Use
    Tags
    EGFP
    Expression
    Mutation
    Y599H and I793S (please see depositor comment bel…
    Promoter
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. mEmerald-TOMM20-N-10

    Plasmid
    #54282
    Purpose
    Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54282-plasmid-map-sequence-id-257386

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  29. pcDNA3.1 mycBioID

    Plasmid
    #35700
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Article
    Roux et al J Cell Biol. 2012 Mar 12.
    Has Service
    Cloning Grade DNA
    Insert
    BirA
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    cmv
    Available since
    April 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    35700-plasmid-map-sequence-id-228311

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  30. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

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  32. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Igor Ulitsky
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141104-plasmid-map-sequence-id-298612

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  33. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV8)

    Viral Prep
    #104492-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Tags
    None
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only

  36. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV immearly promotor
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

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  38. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid
    #85452
    Purpose
    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
    Depositor
    Aviv Regev
    Article
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    85452-plasmid-map-sequence-id-173969

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  40. pBad-mKalama1

    Plasmid
    #14892
    Purpose
    Bacterial expression of mKalama1 blue fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Biochemistry. 2007 May 22;46(20):5904-10
    Insert
    mKalama1
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Promoter
    Available since
    May 15, 2007
    Availability
    Academic Institutions and Nonprofits only

  41. TFORF2069

    Plasmid
    #141902
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYC (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141902-plasmid-map-sequence-id-389798

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  50. pLenti CMVtight Puro DEST (w768-1)

    Plasmid
    #26430
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Lentiviral; Tet-on advanced destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 17, 2010
    Availability
    Academic Institutions and Nonprofits only
    26430-plasmid-map-sequence-id-12473

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Showing: 4651 - 4700 of 20401 results