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31,059 results

Showing: 5151 - 5200 of 31059 results

  2. Traptavidin-E6

    Plasmid
    #59549
    Purpose
    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Core Traptavidin-E6
    Tags
    E6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

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  13. pGEX-2T-Rac1-Q61L

    Plasmid
    #12979
    Depositor
    Gary Bokoch
    Insert
    Rac1 constitutively active (RAC1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Q61L Constitutively active
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  24. p405GAL1

    Plasmid
    #15963
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405GAL1 (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15963-plasmid-map-sequence-id-8448

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  26. pBad-mAmetrine

    Plasmid
    #18083
    Purpose
    Bacterial expression of mAmetrine fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1
    Insert
    mAmetrine fluorescent proteins
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Available Since
    May 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  32. CYP2C19

    Plasmid
    #69606
    Purpose
    For protein expression of CYP2C19
    Depositor
    Joyce Goldstein
    Insert
    CYP2C19 (CYP2C19 Human)
    Expression
    Bacterial
    Mutation
    deletion of AA1-8 and mutation I331V
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69606-plasmid-map-sequence-id-193146

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  33. p0857 pBR322 SV40

    Plasmid
    #10877
    Depositor
    Peter Howley
    Insert
    SV40
    Expression
    Bacterial
    Available Since
    Oct. 26, 2005
    Availability
    Academic Institutions and Nonprofits only

  34. NUDT3A

    Plasmid
    #42348
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT3A (NUDT3 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  45. pET24a-LaM4-EGFP

    Plasmid
    #182641
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    Tags
    BAP, EGFP, HA, His6, and T7
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182641-plasmid-map-sequence-id-358626

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  50. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Article
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

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Showing: 5151 - 5200 of 31059 results