29,729 results
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Plasmid#89698Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only
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pRSET FLIPPi-5u
Plasmid#13554DepositorInsertFLIPPi S52A
UseTagsECFP and EYFPExpressionBacterialMutationS52A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pMRE-Tn7-156
Plasmid#118570PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET 15b-UbcH5a/2417
Plasmid#11472DepositorInsertUbcH5a (UBE2D1 Human)
UseTagsExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p40PX(aa1-148)
Plasmid#19008DepositorInsertp40phox (NCF4 Human)
UseTagsGSTExpressionBacterialMutationaa1-148 (PX domains)PromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE3G-SOX17
Plasmid#104541PurposePiggyBac vector encoding doxycycline-inducible human SOX17DepositorInsertSRY-box 17 (SOX17 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterTRE3GAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-araC
Plasmid#62563PurposeArabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
SND1 (SND1A-c003)
Plasmid#98242PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSND1A (SND1 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00567:TUDOR 677-799PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET303-colE1TR
Plasmid#155181PurposeExpresses colicin E1 residues 1-364DepositorInsertcolicin E1 residues 1-364
UseTagsC-terminal 6x Histidine tagExpressionBacterialMutationtruncation of colicin E1 including 1-364PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSU30
Plasmid#173830PurposeExpresses 30 kD Penn State ladder proteinDepositorInsertHSTPABPACS100BCBP
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU10
Plasmid#173827PurposeExpresses 10 kD Penn State ladder proteinDepositorInsertSTRHSTPAB
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
K84M kinase-dead Nuak1-3xflag
Plasmid#97219PurposePlasmid expressing kinase-dead mutant Nuak1DepositorInsertNuak1 (NUAK1 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationKinase-dead K84MPromoterT7Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR62
Plasmid#125076PurposeExpression of ompR under PLtetO-1, output promoter PompF146DepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTagJr-MBP
Plasmid#105628PurposePlasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigaseDepositorInsertSnoopTagJr-MBP
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pEAT8-137 M1V
Plasmid#173807PurposeExpression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cellsDepositorInsertSERPiNA1 (SERPINA1 Human)
UseTagsExpressionBacterialMutationE1M Otherwise this plasmid encodes the most commo…PromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSDH5_G78R
Plasmid#113047PurposeExpression of mutant hSDH5DepositorInserthSDH5_G78R (SDHAF2 Human)
UseTagsExpressionBacterialMutationG78R, Insert begins at amino acid 39PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPd_PepTSt
Plasmid#58333PurposeExpresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTSt
UseTagsGFP-HisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-dCas9
Plasmid#62244Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.DepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationcatalytically inactive, D10A, H840APromoterPtetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDuex2-iBB-GECO1
Plasmid#194216PurposeFar-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.DepositorInsertiBB-GECO1
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PARP9-c024
Plasmid#173096PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP9 (PARP9 Human)
UseTagsmCeruleanExpressionBacterialMutationG102-K298PromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWP89
Plasmid#61264PurposeIncP-based replicating vector expressing dTomato under tac promoter.DepositorInsertPtac-dTomato
UseSynthetic BiologyTagsExpressionBacterialMutationPromotertacAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pXD70LacZ2-Pdadh-LacZ
Plasmid#191618PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOSV805
Plasmid#126598Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEThT-SAHS1
Plasmid#90025PurposeTo express His-tagged SAHS1 in bacteriaDepositorInsertSAHS1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP2834
Plasmid#160673PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG25-pBAD-tetO-deGFPssrA
Plasmid#102452PurposeExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.DepositorInsertdeGFP
UseTagsssrAExpressionBacterialMutationPromoterpBAD-tetOAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNP561
Plasmid#222723PurposeTarget and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 RecombinaseDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-mBoNT/F
Plasmid#168015PurposeExpresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cellsDepositorInsertMBP-BoNT/F-LC(1-428)-6xHis
UseTagsHisx6 and maltose binding proteinExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GPD1
Plasmid#83911Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
SRF WT pGEX4T
Plasmid#121100PurposeExpression of SRF as a GST fusion proteinDepositorInsertSRF (SRF Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pTD103aiiA
Plasmid#48884PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pMAL-p4X
Plasmid#75289PurposeExpresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneUseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceJuly 19, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
Plasmid#52053PurposeUsed in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromotertetR/pLtetOAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 catalytic
Plasmid#227201PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationaa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-ZF5.3-GFP-HiBit
Plasmid#170992PurposeFor bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-slpGFP
Plasmid#27171DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pASR103
Plasmid#122637PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-TAT-GFP-HiBit
Plasmid#170991PurposeFor bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
FsC_pET29b
Plasmid#215405PurposeExpression construct for FsC gene, codon optimized for expression in E. coliDepositorInsertFsC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSDH5_C83D
Plasmid#113048PurposeExpression of mutant hSDH5DepositorInserthSDH5_C83D (SDHAF2 Human)
UseTagsExpressionBacterialMutationC83D, Insert begins at amino acid 39PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET22-Nsp10-TEV-6His
Plasmid#154957PurposeExpresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsertNsp10-TEV-6His (ORF1ab SARS-CoV-2)
UseTagsTEV-6HisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-2T FRB
Plasmid#26607DepositorInsertmTOR (MTOR Human)
UseTagsGSTExpressionBacterialMutationFRB domain only (aa 2015-2114)PromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-CreiLOV
Plasmid#192817PurposeExpresses CreiLOV in bacteriaDepositorInsertCreiLOV
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE2c
Plasmid#17462Purposemodified pENTR vector for C-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only