32,656 results
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Plasmid#188252PurposeIntegrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only
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pET28a T7pCONS TIR-1 sfGFP
Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
RARS_pNIC-Bio3
Plasmid#153055PurposeExpression construct for producing Avi tagged Arginyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
EPB41L3A-c000
Plasmid#175458PurposeProtein expression in bacterial cells. Entire CDS, M1-D1087.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
JMJD2A
Plasmid#38846PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA(Cm)
Plasmid#48886PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGS140_6xHis-EGFP-I3-01
Plasmid#211889PurposeBacterial expression of I3-01DepositorInsertI3-01
ExpressionBacterialMutationN/AAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG002 (BREX type I)
Plasmid#157880PurposeExpresses the BREX type I defense systemDepositorInsertBREX type I
ExpressionBacterialAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaBC (pCLK1210)
Plasmid#50448PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylaseDepositorInsertPPCS PPCDC
Tags6XHIS tagExpressionBacterialPromoterT7Available SinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas9CyMutL
Plasmid#166981PurposeEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repairDepositorInsertsUseCRISPR and Synthetic BiologyExpressionBacterialMutationE32KPromoterpCymRC and pLacIAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-RBCS-tagRFP
Plasmid#213868PurposeBinary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertRIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
ExpressionBacterial and PlantAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM1s3TsR
Plasmid#137922PurposeE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZ853
Plasmid#208115PurposemSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSDDepositorInsertmNeonGreen-HPRT1-BSD
TagsT2AExpressionBacterial and MammalianPromoterEF1alphaAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500
Plasmid#45788DepositorInsertdeGFP
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterpBAD-tetO1Available SinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRuby3
Plasmid#104004PurposePlasmid encoding mRuby3DepositorInsertmRuby3
UseLow copyExpressionBacterialMutationMutations relative to eqFP611 (MSKGEE LIKENMRMKVV…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pN3
Plasmid#233520PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and HygroR (yeast), Cen6/ARSDepositorTypeEmpty backboneExpressionBacterial and YeastPromoterpGAL1Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
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poly H35S SUMO1
Plasmid#221071PurposeExpression of poly H35S SUMO1 protein under T7 promoterDepositorInsertFive repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)
TagsHis and MBPExpressionBacterialMutationH35SPromotertacAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
polySIM
Plasmid#221068PurposeExpression of polySIM protein under T7 promoterDepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4
TagsHis and MBPExpressionBacterialPromoterunknownAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pCDFDuet-1:ChrH-ChrI
Plasmid#225083PurposeExpresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coliDepositorInsertChrH
ExpressionBacterialMutationWTPromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-155
Plasmid#185939PurposeHimar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein geneDepositorInsertmScarlet-I
ExpressionBacterialAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-157
Plasmid#185940PurposeHimar transposon delivery plasmid that delivers a green fluorescent mClover3 protein geneDepositorInsertmClover3
ExpressionBacterialAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-143
Plasmid#185932PurposeHimar transposon delivery plasmid that delivers a yellow fluorescent sYFP2 protein geneDepositorInsertsYFP2
ExpressionBacterialAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_BRD2-Halo
Plasmid#175336PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-624-919
Plasmid#104200PurposeExpresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion proteinDepositorAvailable SinceDec. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1
Plasmid#89961PurposeModified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.DepositorInsertCas9HF1
ExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet42c(+)-GST-USP5
Plasmid#61750Purposeexpress N-terminally GST tagged human USP5DepositorAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
USP04
Plasmid#25223PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pScaf-5544.4
Plasmid#111524PurposepScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v4)DepositorInsertpScaf-KH'
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSum-LacZ-gfp
Plasmid#109285PurposeExpresses gfp in mycobacteriaDepositorInsertGFP
ExpressionBacterialMutationGFP modified for expression in prokaryotes; inclu…PromoterLacZalphaAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ptetO::bac::gfp
Plasmid#217882Purposeexpresses the bac-BGC in bacterial cells, produces bacillamide DDepositorInsertsDehydrogenase
Non-ribosomal peptide synthetase
Aminotransferase
ExpressionBacterialPromoterptetOAvailable SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pRDV5 containing Sb-convex
Plasmid#132696PurposepRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curveDepositorInsertSb_convex including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-StefinA
Plasmid#182405PurposeBacterial expression of Vesicle Nucleating peptide15-stefinA fusionDepositorInsertVNp15-stefin (CSTA Human)
TagsVesicle Nucleating peptide 15 (VNp15)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDawn-Ag43
Plasmid#107742PurposeExpresses Ag43 under the control of a blue-light transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpLambdaAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21 E2F1
Plasmid#90313PurposeExpresses human E2F1 in bacteriaDepositorAvailable SinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Lac-dCas9 (Ptac)
Plasmid#105234PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescensDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599AAvailable SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJTM-T7-mNeongreen-6xHis
Plasmid#210868PurposePlasmid used for expressing his--tagged mNeongreen for protein purification.DepositorInsertmNeongreen-6xHistag
ExpressionBacterialPromoterT7Available SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3-FGFR1
Plasmid#222611PurposeExpression of MAC3-tagged FGFR1DepositorAvailable SinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-11-LECT2-I40
Plasmid#183032PurposeExpression of LECT2 I40 variant protein from T7 promoterDepositorAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-FimH-9xHis
Plasmid#97305PurposeE.coli UTI89 FimH signal peptide and lectin domainDepositorInsertType 1 fimbiral adhesin FimH
Tags9xHisExpressionBacterialAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP3665
Plasmid#222351PurposeExpression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMO9075
Plasmid#117481Purposeempty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecRDepositorInsertAph(3')-IIa promoter
ExpressionBacterialPromoterAph(3')-IIa promoterAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbS8a-RFP
Plasmid#35274DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterBADAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsR_TS-E10
Plasmid#137944PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expressionDepositorInsertsmRFP1
mWasabi
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101 and pTlpA-C7Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
TagsAvi and HisExpressionBacterialPromoterpBADAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEasyCas9
Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRExpressionBacterial and YeastAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only