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31,059 results

Showing: 5551 - 5600 of 31059 results

  3. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

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  7. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid
    #177722
    Purpose
    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFluc
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177722-plasmid-map-sequence-id-352603

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  8. pET15b-ACPs (from S. pneumonia)

    Plasmid
    #63687
    Purpose
    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae
    Depositor
    Michael Johnson
    Insert
    acpS
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. KAT2B

    Plasmid
    #39002
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KAT2B (KAT2B Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pBlueKan+cysMPro

    Plasmid
    #187879
    Purpose
    Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    CysM
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187879-plasmid-map-sequence-id-368417

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  22. PRDX6A-c001

    Plasmid
    #175474
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX6 (PRDX6 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    See Depositor Comments
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175474-plasmid-map-sequence-id-353175

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  29. pSUB11

    Plasmid
    #169699
    Purpose
    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    Tags
    3xFLAG tag
    Expression
    Bacterial
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169699-plasmid-map-sequence-id-336760

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  30. pUS212

    Plasmid
    #84580
    Purpose
    E.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.
    Depositor
    Nicholas Coleman
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Nov. 21, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    84580-plasmid-map-sequence-id-166638

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  31. pGEX6P1-3xMBT_D355N

    Plasmid
    #46988
    Purpose
    Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli
    Depositor
    Or Gozani
    Article
    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:
    Insert
    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    Tags
    GST and PreScission cleavage site
    Expression
    Bacterial
    Mutation
    Mutation aspartic acid 355 to asparagine (D355N);…
    Promoter
    tac
    Available Since
    Sept. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    46988-plasmid-map-sequence-id-66963

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  34. pExp-mCherry

    Plasmid
    #112569
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and mCherry
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112569-plasmid-map-sequence-id-217213

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  35. EIF5A

    Plasmid
    #25260
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    eIF5A:S15-A151 (EIF5A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S15-A151
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. pGDP4 ermC

    Plasmid
    #112884
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    ermC (ermM Staphylococcus epidermis)
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    Plac
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112884-plasmid-map-sequence-id-219861

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  46. Clomeleon

    Plasmid
    #203723
    Purpose
    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration
    Depositor
    George Augustine
    Article
    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1
    Insert
    cyan fluorescent protein linked yellow florescent protein
    Expression
    Bacterial and Mammalian
    Mutation
    Cyan Fluorescent Protein, Topaz Fluorescent Prote…
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203723-plasmid-map-sequence-id-403632

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  47. pPGN-C

    Plasmid
    #174578
    Purpose
    pOPIN-GG expression vector compatible with N-terminal tagging, carbenicillin resistant
    Depositor
    Mark Banfield
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174578-plasmid-map-sequence-id-344737

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Showing: 5551 - 5600 of 31059 results