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32,656 results

Showing: 5551 - 5600 of 32656 results

  2. KSP_K406

    Plasmid
    #129771
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129771-plasmid-map-sequence-id-263572

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  4. EBFP2-pBAD

    Plasmid
    #54542
    Purpose
    Localization: Protein Expression Vector , Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and EBFP2
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

    Plasmid
    #128164
    Purpose
    UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
    Use
    Synthetic Biology
    Tags
    FLAG and zif268
    Expression
    Bacterial
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128164-plasmid-map-sequence-id-267570

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  13. 6xHis-MBP-huBsCas12a

    Plasmid
    #174671
    Purpose
    Expresses 6xHis-MBP tagged BsCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huBsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    174671-plasmid-map-sequence-id-364005

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  20. pBAD-pHScarlet

    Plasmid
    #191258
    Purpose
    Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet
    Depositor
    Robert Campbell
    Insert
    pHScarlet
    Tags
    His tag
    Expression
    Bacterial
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191258-plasmid-map-sequence-id-374867

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  25. pCE41

    Plasmid
    #174433
    Purpose
    C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174433-plasmid-map-sequence-id-349717

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  27. pTriEx-mVenus-PA-Rac1

    Plasmid
    #22007
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  32. pMBEC2

    Plasmid
    #187596
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187596-plasmid-map-sequence-id-367535

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  34. pBHM2404

    Plasmid
    #173441
    Purpose
    pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    mNeonGreen-CBP-2XFLAG-NAT1
    Tags
    CBP-2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    173441-plasmid-map-sequence-id-341226

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  38. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid
    #128163
    Purpose
    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    ERBIN PDZ domain (ERBIN Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG and Omega subunit of RNA polymerase
    Expression
    Bacterial
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128163-plasmid-map-sequence-id-267568

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  41. pETM33_Nsp10

    Plasmid
    #156479
    Purpose
    Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp10 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156479-plasmid-map-sequence-id-307731

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  49. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

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Showing: 5551 - 5600 of 32656 results