29,740 results
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Plasmid#51056PurposeExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP12049
Plasmid#222355PurposeTn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPabstBRAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHA1
Plasmid#85709PurposeThis plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oPent
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6-PRC1
Plasmid#69111PurposeFor bacterial expression of recombinant human PRC1 tagged with His.DepositorInsertProtein Regulator of Cytokinesis 1 (PRC1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP2B_LAP
Plasmid#101848PurposeTet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCHMP2B (CHMP2B Human)
UseFlp-frt recombination, tet-inducible expressionTagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationsilent mutation in Thr 104; A to TPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-TACC3-His6
Plasmid#69106PurposeFor bacterial expression of recombinant human TACC3 tagged with GST and His.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseTagsGST and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMP220
Plasmid#185921PurposeBroad host range IncP promoter probe using lacZDepositorTypeEmpty backboneUseIncpTagsLacZExpressionBacterialMutationPromoterNoneAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CTH10STII-GG
Plasmid#215804PurposeCloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pHEN-CoV2-nanobody-F1
Plasmid#194592PurposeBacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertF1 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYZ205
Plasmid#104586PurposeExpress SNAP-6His-CBDDepositorInsertSNAP-6His-CBD
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCM433kanT
Plasmid#61262PurposeSucrose counterselection (sacB) vector for unmarked allelic exchange.DepositorTypeEmpty backboneUseSynthetic Biology; Sucrose counterselectionTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110.i1
Plasmid#135481PurposeE. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110.i1
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQR
Plasmid#48099PurposeCumate-inducible expression of mCherry-tagged fusion proteinDepositorTypeEmpty backboneUseTags3xFLAG and mCherryExpressionBacterialMutationPromoterPQ5Available sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDonorCm(Ptr)
Plasmid#175577PurposeDNA donor for transpositionDepositorInsertMini-Tn, Cm cassette
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIV296
Plasmid#204687PurposeAMA1 plasmid with nat1 resistance markerDepositorInsertnat1
UseFungal expressionTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
884 pGEX2T PTEN G129E
Plasmid#10747DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionBacterialMutationG129EPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pETM6-mCherry
Plasmid#66534PurposeExpresses mCherry under the control of the Concensus T7 PromoterDepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterConsensus T7Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-2H12.D11
Plasmid#221145PurposeHis6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)DepositorInsertcdGreen2
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)/FaGH16A
Plasmid#100910PurposeExpression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coliDepositorInsertFaGH16A
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
evoFERNY-Cas9NG
Plasmid#203329PurposePlasmid for bacterial purification of codon optimized evoFERNY-Cas9NGDepositorInsertevoFERNY-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Smad3
Plasmid#12630DepositorInsertSmad3 (SMAD3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
6BYB (L3MBTL1)
Plasmid#119957PurposeBacterial expression for structure determinationDepositorInsertL3MBTL1 (L3MBTL1 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pstb-LAFR5
Plasmid#86169PurposepLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid vector with double cos sites.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSMb21651 promoterAvailable sinceFeb. 6, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBLxVi5
Plasmid#167516PurposeBroad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, Violacein, Gentamicin Resistance Marker.DepositorInsertLuxR-Violacein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPTM p62 PB1 (1-102)
Plasmid#210427PurposeBacterial expression of MBP-PB1(1-102) with TEV cleavage siteDepositorInsertp62 PB1 (1-102) (NUP62 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB167
Plasmid#101672PurposeOpto-T7RNAP*(563), equal expr.DepositorInsertOpto-T7RNAP*(563), equal expr.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0310:Dendra
Plasmid#159051PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0310
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28Teb1
Plasmid#28173DepositorInsertTeb1
UseTags6xhistidineExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
JpExpress404 PTEN-LONG
Plasmid#49417PurposeBacterial Expression VectorDepositorInsertPTEN-Long (PTEN )
UseTagsExpressionBacterialMutationPromoterT5Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKB817 (pBra-Hfq)
Plasmid#174661PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.DepositorInsertHfq
UseTagsalpha NTDExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)GFP-9xGGS-Cre-6xHis
Plasmid#62372PurposeExpression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cellsDepositorInsert(-30)GFP-9xGGS-Cre-6xHis
UseTags(-30)GFP and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdTomato
Plasmid#163443PurposeBacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backboneDepositorInserttdTomato
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB083
Plasmid#181952PurposeIPTG-inducible expression of PhoQDepositorInsertPhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
UseTagsExpressionBacterialMutationPromoterPhoQ-Ptac; lacI-PlacIqAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pHCL151
Plasmid#134455PurposeTetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.DepositorInsertlinker-mScarlet-I for C-terminal fusion
UseTagsExpressionBacterialMutationPromoterplacAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pATLIC
Plasmid#110287PurposeA backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)DepositorInsertyfaL
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-3WJdB-T
Plasmid#87308PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.DepositorInsertThree-way junction dimeric Broccoli aptamer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 Mac1 - P43 (Oxford)
Plasmid#169210PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PIA586
Plasmid#104399Purposevector for expression of single E.coli RNAP subunitDepositorInsertrpoD
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSucCD2
Plasmid#83324Purposeexpresses E. coli succinyl-CoA synthetaseDepositorInsertsucCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
FtsZ
Plasmid#74128PurposeExpresses FtsZ without a peptideDepositorInsertFtsZ
UseTagsC-terminal linker sequence (G G S G S G S S)ExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-eGFP-6xHis
Plasmid#130981PurposeBacterial expression of mouse EB1-GFP-6xHis for protein purification.DepositorInsertEB1-GFP-6xHis (Mapre1 Mouse)
UseTagseGFP-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-mGold
Plasmid#218959PurposeExpresses Hisx6-Tagged mGold in bacteriaDepositorInsertmGold
UseTags6X-HisExpressionBacterialMutationPromoterT5Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
PARP1-c009
Plasmid#216872PurposeBacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-Cdc42-wt
Plasmid#12969DepositorInsertCdc42 (CDC42 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM2
Plasmid#32882DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 497-566PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
Twin-Strep(R)-Tev-GSGS-Ubiquitin
Plasmid#186803PurposeCodon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.DepositorInsertTwin-Strep(R)-Tev-GSGS-Ubiquitin
UseTagsGS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…ExpressionBacterialMutationStrep tag Lysine's mutated to ArgininePromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only