31,059 results
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Plasmid#131609Purposeexpression of mouse Runx1 in mammalian cellsDepositorAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only
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hnRNPA2_RRMs
Plasmid#157864Purposeexpress His tagged hnRNPA2 RRMsDepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbE0k-RFP
Plasmid#35365DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDLxR6
Plasmid#172560PurposeInducible gene expression, pCDF origin, LuxR activator, PLuxB promoter, mRFP, Streptomycin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT137-splitD
Plasmid#122606Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
ExpressionBacterialAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMTEZ-Kir2.1
Plasmid#32641DepositorInsertKir2.1 (Kcnj2 Mouse)
ExpressionBacterialAvailable SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TAA
Plasmid#208187Purposenonsense mistranslation, TAA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialMutationInsertion 6TAA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG86-J23151-LasR
Plasmid#102456PurposeExpresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.DepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pYTK-DN6
Plasmid#180287PurposeHygR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - HygR - CEN6/ARS4
ExpressionBacterial and YeastAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1
Plasmid#164614PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.DepositorInsertstdMCP-stdHalo-RH1
UseLentiviralTagsHA tag and NLSExpressionBacterial and MammalianPromoterUbiCAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPTDH
Plasmid#66183PurposeExpresses phosphite dehydrogenase in E. coliDepositorInsertPhosphite Dehydrogenase
TagsHis6 tagExpressionBacterialMutationE175A, A176RPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBI (JBEI-3085)
Plasmid#87950Purposethe overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).DepositorInsertMevT-MBI
ExpressionBacterialAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-NGFP
Plasmid#52734PurposeIPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assayDepositorInsertNGFP+Z-fusion
TagsHis tagExpressionBacterialPromoterT7Available SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(40nm)-Cys
Plasmid#91571PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(40nm)-Cys
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-HNRNPA1LCD
Plasmid#204405PurposeE. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI
Plasmid#115220PurposeExpresses Pin3Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin3Cas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE-BR1
Plasmid#160333PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.DepositorInsertBR1
Tags6xHis tagExpressionBacterialPromoterT5Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
TagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alanineAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Rx3(A7)
Plasmid#35525DepositorInsertHis6-Rx3(A7)
TagsHis6ExpressionBacterialMutationChanged G2 to PPromoterT7Available SinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQmod2C-GG
Plasmid#191347PurposeClostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJA48 HLA-B*57:01
Plasmid#180444PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*57:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRAU168
Plasmid#86834Purposebacterial expression of anti-CRISPR protein AcrIIA1DepositorInsertacrIIA1
ExpressionBacterialPromoteraraBADAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTmpSm
Plasmid#176228PurposeA template plasmid for amplification of the cI-hok-strA cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, strA
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI
Plasmid#115210PurposeExpresses AspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertAspCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP
Plasmid#187113PurposeProtein ExpressionDepositorAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-DARPinOFF7
Plasmid#182415PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusionDepositorInsertVNp-mNeongreen-DARPinOFF7
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET20b-NLuc-MBP-6xHis
Plasmid#135931PurposeExpresses NanoLuciferase in bacterial cells.DepositorInsertNanoLuciferase
TagsMBP-6xHisExpressionBacterialPromoterT7Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLC-246
Plasmid#73190PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-tse2
ExpressionBacterialPromoterPrhaBAvailable SinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6
Plasmid#174498Purposebacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6DepositorTagsHis6-TEV and msfGFPExpressionBacterialAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBMCS-SortA
Plasmid#21931DepositorInsertsortase (srtA Staphylococcus aureus (strain N315))
TagsGB1 and His-tagExpressionBacterialMutationdeleted amino acids 1-59Available SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSLC-284
Plasmid#124294PurposeA low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
ExpressionBacterialAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-tdTEVDB_C-15A/G4C
Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
Tags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-cdaR
Plasmid#62557PurposeGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.DepositorInsertsbeta lactamase
sfGFP
CdaR
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEN6-Ubc5 Sc
Plasmid#83234PurposepHis-par2-UbHs/Ubc5Sc/ Uba1TaDepositorAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 vatD
Plasmid#112885PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertvatD
ExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 vph
Plasmid#112893PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertvph
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3-NCwt-SYFP2
Plasmid#189766PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet3-NCwt-SYFP2
Tags6xHisExpressionBacterial and MammalianMutationencodes the expected internal mutations relative …PromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-L11
Plasmid#127831PurposeProduction of recombinant human ribosomal protein L11DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRα
Plasmid#53731PurposePlpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertαNTD
ExpressionBacterialPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBTL-2
Plasmid#22806DepositorInsert
ExpressionBacterialAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQE-meGFP-DLC1
Plasmid#192048PurposePlasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFPDepositorInsertDLC1 (DLC1 Human)
ExpressionBacterialAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-ATP
Plasmid#225247PurposeBacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-Rab27a
Plasmid#194985Purposeoverexpress Rab27aDepositorInsertGFP-Rab27a
ExpressionBacterialAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pELlR4
Plasmid#172545PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Carbenicillin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEPZ-Plac
Plasmid#122635PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC101-ΔPS-PM,CS-RBSBBa_B0034-geneVI
Plasmid#134356PurposeAccessory plasmidDepositorInsertGene VI
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pCadBA-mCherry
Plasmid#192865PurposepMUT2 based vector for sensing pH in E. coli Nissle 1917DepositorInsertpH sensitive promoter
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
SIRT4.23
Plasmid#13737DepositorAvailable SinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJMP2832
Plasmid#160672PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
ExpressionBacterialAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only