31,059 results
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Plasmid#122662PurposeExpress tUI-HA protein in E. coliDepositorInserttUI-HA
TagsHA-tag and His-tagExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXB1-EXO1b
Plasmid#68267PurposeBacterial expression of human EXO1DepositorInsertEXO1 (EXO1 Human)
TagsMxe intein/chitin binding domainExpressionBacterialMutationb form, codons 1-846. Please see depositor commen…PromoterT7Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attR4-pegRNA-attR3
Plasmid#213056PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attL4
Plasmid#213055PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*-XcP4H-MH4R
Plasmid#178043PurposeExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTPDepositorInsertssuperfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human
TagsHis tagExpressionBacterialAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRBV6-5
Plasmid#123425Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP3535VA
Plasmid#46887PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…ExpressionBacterialPromoterPnisAAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR3C2_Hormone-recep
Plasmid#109848PurposeProtein expression and purification of NR3C2_Hormone-recepDepositorInsertNR3C2_Hormone-recep (NR3C2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.2
Plasmid#171201PurposeExpresses Br512g3.2 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialPromoterT7Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-3nmt1
Plasmid#105162PurposeYeast gene targetingDepositorInserthphMX6-3nmt1
Tags3nmt1ExpressionBacterial and YeastPromoter3nmt1Available SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
ExpressionBacterialPromotertetpAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His6-ODC-Ctag
Plasmid#163614PurposeExpresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.DepositorAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1185
Plasmid#119255Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
mT-Sapphire-pBAD
Plasmid#54571PurposeLocalization: Protein Expression Vector , Excitation: 399, Emission: 511DepositorTypeEmpty backboneTags6xHis and mT-SapphireExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pA5c-tesA-vhb50-8fadR
Plasmid#86601Purposeexpression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoterDepositorInsertstesA
Vhb50
fadR
ExpressionBacterialPromoterLacUV5 and pBADAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS6k-RFP
Plasmid#35294DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterLlacO-1Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRM049 His-TEV-Cas9 triNLS 2C
Plasmid#196245PurposeExpression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF3
Plasmid#228777PurposeProtein expression plasmid for YTH domain of YTHDF3 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046959
Plasmid#145722PurposeBacterial Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEXndoS
Plasmid#44655DepositorAvailable SinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Cre-6xHis
Plasmid#62939PurposeExpression of Cre-6xHis in bacterial of cellsDepositorInsertCre recombinase
Tags6xHis tagExpressionBacterialPromoterT7Available SinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
Plasmid#200277PurposeCharacterisation of the sialic acid-responsive circuit with fluorescent reporterDepositorInsertsCadC
NanR
ExpressionBacterialAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IRP1-Halo
Plasmid#175305PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIV297
Plasmid#204820PurposeAMA1 plasmid with amdS selection markerDepositorInsertamdS
UseFungal expressionExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans trpC promoterAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNGFP-BC
Plasmid#161956PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneTagsGFPuv His8ExpressionBacterialPromoterT7Available SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCGFP-BC
Plasmid#161959PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneTagsGFPuv His8ExpressionBacterialPromoterT7Available SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB-Tl-ADPHK
Plasmid#237469PurposeExpression of His-tagged Thermococcus litoralis ADP hexokinaseDepositorInsertADP hexokinase
TagsC-terminal 10 His-tag and N-terminal strep tagExpressionBacterialPromoterT7Available SinceJune 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETCK3 TGP-E
Plasmid#236255PurposeExpresses TGP-E, a thermostable fluorescent proteinDepositorInsertThermal Green Protein Q66E (TGP-E)
TagsHis tagExpressionBacterialPromoterlac operonAvailable SinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2WWE
Plasmid#234210PurposeHeterologous protein expression of PI3K-C2-gamma in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1
Plasmid#184556PurposeKnockdown of murine KRAS gene by targeting the promoter region via CRISPRi systemDepositorInsertKirsten rat sarcoma virus (Kras Mouse)
UseCRISPR, Lentiviral, and Mouse TargetingTagsFlagExpressionBacterialAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving
Plasmid#204811PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
ExpressionBacterialAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
BPK2014-Cas12i-Max
Plasmid#188276Purposeprokaryotic expressionDepositorInsertCas12i-Max
UseCRISPRExpressionBacterialMutationnonePromoterT7Available SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUS250
Plasmid#198322PurposeCumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoproteinDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromotercumate-inducibleAvailable SinceApril 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZE-PalkB-GFP
Plasmid#44381DepositorInsertsGFP
PalkB
UseSynthetic BiologyExpressionBacterialPromoterPalkBAvailable SinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pspCas9-Klenow-ST2-com-vector
Plasmid#176235PurposeVector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse cyclin D2
Plasmid#86791Purposebacterial expression of mouse cyclin D2DepositorInsertcyclin D2 (Ccnd2 Mouse)
ExpressionBacterialAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTW357b
Plasmid#227930PurposeLow-stringency accessory plasmid for selectionDepositorInsertsgIII (recoded)
T7c
ExpressionBacterialMutationR632SAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO PPM1H
Plasmid#207333PurposeBacterial expression of His SUMO PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2attB-FST344-HA-CPL
Plasmid#131460PurposeEncodes human Follistatin proteinDepositorAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMyCA
Plasmid#84268PurposeA versatile vector for recombinant mycobacterial protein productionDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAceteamidaseAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG019 (AVAST type 3)
Plasmid#157897PurposeExpresses the AVAST type 3 defense systemDepositorInsertAVAST type 3 (STAND ATPase with N-terminal DUF4297)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
ExpressionBacterialAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CTTN-1/1
Plasmid#91550PurposeProtein expression and purification of human SH3 domain construct CTTN-1/1DepositorInsertCTTN-1/1 (CTTN Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CH
Plasmid#26117PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTagsHisExpressionBacterialPromoterT7-lacOAvailable SinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorInsertLasI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-ELP[AV-60]
Plasmid#67012PurposeExpresses malE-ELP[AV-60] in E. coliDepositorInsertmalE-ELP[AV-60]
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only