Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

Your Selections

Bacterial Expression

29,744 results

Showing: 5901 - 5950 of 29744 results

  1. RAB5B

    Plasmid
    #25251
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB5B:A15-G191 (RAB5B Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids A15-G191
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. pEF1alpha-mClover3-bGHpA

    Plasmid
    #172286
    Purpose
    Constitutive expression of mClover3
    Depositor
    Patrik Verstreken
    Insert
    mClover3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    EF1alpha
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172286-plasmid-map-sequence-id-340305

    Enlarge

  4. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid
    #192349
    Purpose
    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    Use
    Tags
    6X-His-Sumo
    Expression
    Bacterial
    Mutation
    Cysteine 145 of the active site is substituted wi…
    Promoter
    T7 lac
    Available since
    June 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    192349-plasmid-map-sequence-id-381344

    Enlarge

  8. pLPE1

    Plasmid
    #209981
    Purpose
    Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    E. coli shuttle part (pMB1_ampR_B0017)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209981-plasmid-map-sequence-id-419436

    Enlarge

  9. pLPE2

    Plasmid
    #209982
    Purpose
    Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    E. coli shuttle part (ampR_B0017)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209982-plasmid-map-sequence-id-419966

    Enlarge

  16. pLPP5

    Plasmid
    #209967
    Purpose
    Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    Promoter (P_sppQ)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209967-plasmid-map-sequence-id-419962

    Enlarge

  17. pLPP6

    Plasmid
    #209968
    Purpose
    Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    Promoter Dropout (sfGFP)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209968-plasmid-map-sequence-id-419374

    Enlarge

  27. pet15b_ack

    Plasmid
    #220229
    Purpose
    Bacterial expression of AcK in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Acetate kinase (ackA )
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220229-plasmid-map-sequence-id-436823

    Enlarge

  37. pJYP1

    Plasmid
    #202406
    Purpose
    Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)
    Depositor
    Remy Loris
    Article
    Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    fdeA
    Available since
    Aug. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202406-plasmid-map-sequence-id-403395

    Enlarge

  41. pID1072

    Plasmid
    #164869
    Purpose
    The pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.
    Depositor
    Keith Shearwin
    Article
    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612
    Insert
    phiKO2 cB repressor
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    164869-plasmid-map-sequence-id-360033

    Enlarge

  42. pUS252y

    Plasmid
    #191834
    Purpose
    Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.
    Depositor
    Nicholas Coleman
    Insert
    fuYFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191834-plasmid-map-sequence-id-382126

    Enlarge

  44. ARF1 (ARF1A-c001)

    Plasmid
    #110258
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ARF1 (ARF1 Human)
    Use
    Tags
    StrepII-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110258-plasmid-map-sequence-id-212329

    Enlarge

  48. AURKB

    Plasmid
    #39119
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AURKB (AURKB Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5901 - 5950 of 29744 results