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32,656 results

Showing: 551 - 600 of 32656 results

  2. pHIS-GB1-BP1

    Plasmid
    #133427
    Purpose
    Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    SREBF1 (SREBF1 Human)
    Expression
    Bacterial
    Mutation
    SREBP1a amino acids 17-42 fused to GB1 solubilit…
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133427-plasmid-map-sequence-id-267726

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  5. Clomeleon

    Plasmid
    #203723
    Purpose
    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration
    Depositor
    George Augustine
    Article
    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1
    Insert
    cyan fluorescent protein linked yellow florescent protein
    Expression
    Bacterial and Mammalian
    Mutation
    Cyan Fluorescent Protein, Topaz Fluorescent Prote…
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203723-plasmid-map-sequence-id-403632

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  6. RGB-S reporter

    Plasmid
    #207841
    Purpose
    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli
    Depositor
    Christof Niemeyer
    Article
    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(
    Inserts
    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    Expression
    Bacterial
    Promoter
    PosmY, PsulA, and grpE
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207841-plasmid-map-sequence-id-426759

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  8. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  24. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Tags
    6xHis-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129593-plasmid-map-sequence-id-254271

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  32. pMAL-c2X_MBP-CLmN-H6

    Plasmid
    #234487
    Purpose
    Maltose binding protein N-terminally fused to His-tagged CLmN split intein
    Depositor
    Henning Mootz
    Article
    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:
    Insert
    N-terminal Fragment of the CLm Split Intein
    Tags
    Hexahistidine Tag and Maltose Binding Protein (MB…
    Expression
    Bacterial
    Mutation
    T69K, F75H, M118N within the N-terminal fragment …
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    234487-plasmid-map-sequence-id-471039

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  33. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  34. pFV2-HA

    Plasmid
    #187082
    Purpose
    Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)
    Depositor
    Steven Vik
    Article
    Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1
    Insert
    atpBEFHAGDC
    Tags
    6-His beta subunit (atpD) and HA tag subunit a (a…
    Expression
    Bacterial
    Mutation
    All Cys codons changed to Ala.
    Available Since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    187082-plasmid-map-sequence-id-372620

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  45. pDB-His-MBP-SARM1

    Plasmid
    #195762
    Purpose
    Purification of SARM1 from E.coli
    Depositor
    Hao Wu
    Insert
    SARM1 (SARM1 Human)
    Tags
    HIS-MBP-TEV
    Expression
    Bacterial
    Mutation
    truncation of first 19 residues
    Promoter
    T7
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195762-plasmid-map-sequence-id-384751

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  49. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid
    #198468
    Purpose
    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.
    Depositor
    Viviana Risca
    Article
    Soroczynski et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    10His-pG-Tn5 (E54K, L372P)
    Tags
    10His-pG
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    Consensus φ10 T7 Promoter
    Available Since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198468-plasmid-map-sequence-id-390256

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Showing: 551 - 600 of 32656 results