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Bacterial Expression

29,747 results

Showing: 6551 - 6600 of 29747 results

  4. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  7. pCK301

    Plasmid
    #87767
    Purpose
    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Insert
    PrhaBAD-sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87767-plasmid-map-sequence-id-177279

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  9. pCoofy44

    Plasmid
    #55185
    Purpose
    Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…
    Expression
    Bacterial
    Mutation
    Promoter
    pPH (Polyhedrin promoter)
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55185-plasmid-map-sequence-id-85559

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  10. pNHEJ-Cas12a-recX

    Plasmid
    #158714
    Purpose
    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    recX
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pmyc
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    158714-plasmid-map-sequence-id-321929

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  14. pPBC-LG3-tdT

    Plasmid
    #62810
    Purpose
    pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    62810-plasmid-map-sequence-id-123118

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  19. SUMO-KTag

    Plasmid
    #51723
    Purpose
    Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SUMO-KTag
    Use
    Tags
    His6 Tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. KIF3A406

    Plasmid
    #129768
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    129768-plasmid-map-sequence-id-271499

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  21. pJJS005

    Plasmid
    #188328
    Purpose
    PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    mIAA7::mNeonGreen::Linker
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188328-plasmid-map-sequence-id-370751

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  23. pET-A3A(N57Q)-BE3

    Plasmid
    #158157
    Purpose
    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS
    Depositor
    Keith Joung
    Article
    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10
    Insert
    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    Use
    Tags
    6xHis tag on N-terminus
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    158157-plasmid-map-sequence-id-309498

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  34. WT-eGFP

    Plasmid
    #215168
    Purpose
    Encodes the enhanced green fluorescent protein
    Depositor
    Bhuvana Shanbhag, Travis Beddoe
    Insert
    WT-eGFP
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215168-plasmid-map-sequence-id-430164

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  36. pLS2965

    Plasmid
    #122534
    Purpose
    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_aman-2::CemOrange2 (aman-2 Synthetic, Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122534-plasmid-map-sequence-id-238120

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  42. 5WCI (MYST1)

    Plasmid
    #101269
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST1 (KAT8 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101269-plasmid-map-sequence-id-193502

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  48. G-SMARCA4-2

    Plasmid
    #166519
    Purpose
    scFv of a human scaffold targeting SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4 isoform B. Antigen coverage aa 1451-1580 of 1647, Bromodomain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166519-plasmid-map-sequence-id-329058

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  49. pZmUbi-5'UTR

    Plasmid
    #154057
    Purpose
    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    Promoter and 5' UTR Ubiquitin (Zea mays)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    Sept. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154057-plasmid-map-sequence-id-313121

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Showing: 6551 - 6600 of 29747 results