31,059 results
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Plasmid#122138PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain imperfect operator sequence (anaerobic purpose)DepositorInsertFBFP
ExpressionBacterialPromoterJ23101Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AK1
Plasmid#39134PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG
Plasmid#155131PurposeExpresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
TagsHisExpressionBacterialMutationinsert codes for GlpG residues 87-276PromoterT7Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q
Plasmid#84352PurposeExpression of the human Huntingtin Exon1 protein containing 43Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pReporter_12
Plasmid#60572PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_12
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG4
Plasmid#70115PurposeTn7-based lux reporter for promoter cloning in bacteriaDepositorTypeEmpty backboneExpressionBacterialPromoternoneAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9-actII-4-NT-S1
Plasmid#110185PurposeRepression of gene expression in Streptomyces by CRISPRiDepositorInsertsdCas9
sgRNA
UseCRISPR; IExpressionBacterialMutationD10A, H840APromoterJ23119 and ermE*pAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-YFP
Plasmid#186563Purposep15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
TagsNoneExpressionBacterial and MammalianMutationNonePromoterPromotorlessAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE147
Plasmid#118499Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmClover3
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
BtuF
Plasmid#92209PurposeExpression of BtuF which is the vitamin B12 binding protein for BtuCD ABC TransporterDepositorInsertBtuF (btuF )
Tags6x His Tag and PelB Signal SequenceExpressionBacterialMutationBtuF signal sequence removed and uses the PelB si…PromoterT7 PromoterAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046852
Plasmid#145584PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM33_Nsp4
Plasmid#156474PurposeBacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tagDepositorInsertNsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-140
Plasmid#118524PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AdML-M3
Plasmid#11244DepositorInsertAdML-M3
Tags3 MS2 hairpinsExpressionBacterialAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-weak
Plasmid#108314PurposeGFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.DepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterpFAB4282Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NFAT5 luciferase
Plasmid#14110DepositorAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
CTV
Plasmid#131590PurposePlasmid for the targeting of chloramphenicol geneDepositorInsertNeomycin resistance gene
ExpressionBacterialAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD Ugi1
Plasmid#177194PurposeGreengate entry vector pGGC000 modified to contain bladderwort insulator 1DepositorInsertUtricularia gibba insulator 1
ExpressionBacterialAvailable SinceJune 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
ETV5_pet28a
Plasmid#131639PurposeBacterial expression of ETS gene ETV5DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16b-MYPT1(1-314)
Plasmid#224893PurposeBacterial expression of MYPT1(1-314)DepositorInsertmyosin phosphatase targeting subunit 1 (PPP1R12A )
ExpressionBacterialAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-MET-IV
Plasmid#218117PurposeMET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-LYS-IV
Plasmid#218118PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL1284
Plasmid#175960PurposeFor expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
Tags6xHis 2xQH and TEV cleavage site on LetAExpressionBacterialPromoteraraBADAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-AR-S256A
Plasmid#171227PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser256Ala (AR-S256A)DepositorInserthuman androgen receptor Ser256Ala mutant (AR Human)
ExpressionBacterial and MammalianMutationAR-Ser256Ala (AR-S256A)PromoterCMVAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-AR-S308A
Plasmid#171229PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser308Ala (AR-S308A)DepositorInserthuman androgen receptor Ser308Ala mutant (AR Human)
ExpressionBacterial and MammalianMutationAR-Ser308Ala (AR-S308A)PromoterCMVAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-AR-S90A
Plasmid#171225PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)DepositorInserthuman androgen receptor Ser90Ala mutant (AR Human)
ExpressionBacterial and MammalianMutationAR-Ser90Ala (AR-S90A)PromoterCMVAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PTBP1-Halo
Plasmid#175302PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
NylCA_pET28a
Plasmid#215418PurposeExpression construct for NylCA gene, codon optimized for expression in E. coliDepositorInsertNylCA
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2_pET28a
Plasmid#215420PurposeExpression construct for NylCp2 gene, codon optimized for expression in E. coliDepositorInsertNylCp2
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLas eGFP
Plasmid#193630PurposeInducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).DepositorInserteGFP
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMYCO1_SpdCas9_pmcDA1
Plasmid#204615PurposeCRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoidesDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-APEX2-F
Plasmid#160083PurposeBacterial expression of C-terminally FLAG-tagged APEX2 peroxidaseDepositorInsertAPEX2 peroxidase
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-62 pComb3xss anti-cFos nanobody
Plasmid#145835PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C1-62 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pKM427
Plasmid#210788PurposeL5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transferDepositorInsertDefective hygromycin resistant gene
ExpressionBacterialMutationChanged hygromycin resistant gene W190 codon (TGG…Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPalkBAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHA1
Plasmid#85709PurposeThis plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningExpressionBacterialAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-3
Plasmid#172769PurposeBacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-3
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV1F(Spec)
Plasmid#205086PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
ExpressionBacterialAvailable SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHK316
Plasmid#235474PurposeInducible gene VIII by Ptet promoterDepositorInsertphage M13 geneVIII
ExpressionBacterialAvailable SinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAROTENE5X
Plasmid#208816PurposeEncodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cellDepositorInsertFive copies of JUB1 binding site within the synthetic promoter
UseSynthetic BiologyExpressionBacterialPromoterJUB1 TF-responsive promoterAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH72
Plasmid#202487PurposeExpresses the nanobody VHH72 with T7 inductionDepositorInsertnanobody VHH72
Tags6XHisExpressionBacterialPromoterT7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-LCC
Plasmid#218686PurposeEncodes codon optimized LCC enzyme for bacterial expressionDepositorInsertLCC
Tags10xHisExpressionBacterialAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCgVchcrRNA
Plasmid#203815PurposeExpression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertCRISPR(BsaI)
UseCRISPRExpressionBacterialAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB256
Plasmid#170039PurposeEncodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Bac2a
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
His_Naa80d in pTYB12
Plasmid#188455PurposeExpress in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.DepositorAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-HBB-nLuc
Plasmid#175432PurposeUsed to generate IVT RNA for translation extractsDepositorInsertHBB-nLuc
UseLuciferaseExpressionBacterialPromoterT7Available SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mNeonGreen
Plasmid#191743PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and mNeonGreenExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJKR-L-tetR
Plasmid#62562PurposeTetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635
Plasmid#91918PurposeExpresses LbfCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLbfCas13a and guide expression cassette
ExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only