32,126 results
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Plasmid#139934PurposeIPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLacI-Ptac-sfGFP
ExpressionBacterialAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_hGH
Plasmid#182410PurposeBacterial expression of human growth hormoneDepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATG-1929
Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
ExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR230
Plasmid#79546PurposeBxbI attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG22-pBAD-TetR
Plasmid#102451PurposeExpresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.DepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDonorCm(Ptr)
Plasmid#175577PurposeDNA donor for transpositionDepositorInsertMini-Tn, Cm cassette
ExpressionBacterialMutationNOAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-134
Plasmid#118520PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter-mCherry
Plasmid#65008PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterUser-definedAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBz15_Strep_Rubisco
Plasmid#140839Purposewild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backboneDepositorInsertForm IA Rubisco; CbbL, CbbS
TagsStrep IIExpressionBacterialPromoterT7Available SinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RANBP2_Ran-BP2
Plasmid#109839PurposeProtein expression and purification of RANBP2_Ran-BP2DepositorInsertRANBP2_Ran-BP2 (RANBP2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
ExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 TRAF
Plasmid#227226PurposeExpresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationaa 62-205PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTHDC1-FL
Plasmid#177121PurposeBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTHDC1 Human)
TagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialPromoterT7Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ208
Plasmid#162681PurposeLidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
TagsHisExpressionBacterialMutationReplacement of W185-F191 with G251-T472 from MHET…PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-eyfp
Plasmid#65039PurposeZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteriaDepositorInserteyfp
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOSV809
Plasmid#126602Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
hsp70:Arl13b-mKate2
Plasmid#105964Purposeheat shock inducible transgenesis, primary cillium markerDepositorAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SPA2G231V
Plasmid#42079DepositorInsertSurfactant Protein -A2 (SFTPA2 Human)
UseLentiviral; PlentiTagsV5ExpressionBacterial and MammalianMutationChanged Glycine 231 to ValinePromoterCMVAvailable SinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPEPY
Plasmid#122633PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 WT
Plasmid#67607Purposebacterial expression of wild type human PRMT9DepositorAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I
Plasmid#208183Purposemistranslation GFP and mScarlet-I positive controlDepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialPromoterPtet+dnaK P1Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SW5
Plasmid#40108DepositorInsertBistable switch using dynamic degradation
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMYCO1_SpdCas9_pmcDA1
Plasmid#204615PurposeCRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoidesDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLC-255
Plasmid#124292PurposeExpression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
ExpressionBacterialAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEPX-Plac
Plasmid#122632PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2
Plasmid#196250PurposePlasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119DepositorInsertattL1-GlpT-scGFP-attL2
ExpressionBacterialPromoterJ23119 promoterAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-2
Plasmid#172768PurposeBacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOGG037
Plasmid#113995PurposeSC module. Super-folder GFP green fluorescence reporterDepositorInsertSuper-folder GFP
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP
Plasmid#134357PurposeReporter plasmidDepositorInsertsGFP
mCherry
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUB7
Plasmid#169698PurposeTemplate plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags6xHis tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPro18
Plasmid#17806DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM22C
Plasmid#135488PurposeAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hphDepositorInserthph SpunH2A/Bpr LifeAct-tdTomato
TagsLifeActExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEK3395
Plasmid#234079PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertYukonOFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
Plasmid#198450PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).DepositorInsertPVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
ExpressionBacterialAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 stat
Plasmid#112886PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertstat
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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MacroH2A1.1-c002
Plasmid#173087PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
TagsmCeruleanExpressionBacterialMutationQ162-N369, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS hBAF53a
Plasmid#17879DepositorInsertBAF53a (ACTL6A Human)
ExpressionBacterialAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA5-Halo
Plasmid#175326PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
6975 pPROEX-HTb-SIS1
Plasmid#1233DepositorAvailable SinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only