Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,059 results

Showing: 951 - 1000 of 31059 results

  9. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Available Since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pM2s2AsG

    Plasmid
    #137924
    Purpose
    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    137924-plasmid-map-sequence-id-331367

    Enlarge

  14. pGEX6P1-DEST-His

    Plasmid
    #119763
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    10xHis and GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119763-plasmid-map-sequence-id-234026

    Enlarge

  24. pTP955

    Plasmid
    #104164
    Purpose
    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104164-plasmid-map-sequence-id-322877

    Enlarge

  30. pGRG36-Kn-PA1-GFP

    Plasmid
    #79088
    Purpose
    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
    Depositor
    Frank Rosenzweig
    Article
    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)
    Insert
    GFP
    Use
    Bacterial chromosome integration and expression o…
    Expression
    Bacterial
    Promoter
    PA1
    Available Since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    79088-plasmid-map-sequence-id-182383

    Enlarge

  31. pCWU50

    Plasmid
    #232318
    Purpose
    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Promoter
    fdx
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232318-plasmid-map-sequence-id-462281

    Enlarge

  36. pEF1-Pls3-sfGFP

    Plasmid
    #205750
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls3 (Phyperspank mutant, IPTG inducible)
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205750-plasmid-map-sequence-id-478438

    Enlarge

  38. OmpF

    Plasmid
    #213135
    Purpose
    Expresses outer membrane protein F
    Depositor
    Sebastian Hiller
    Insert
    OmpF (23-362) (ompF E.coli (K12))
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213135-plasmid-map-sequence-id-422154

    Enlarge

  40. pDx_mScarlet3-H

    Plasmid
    #242970
    Purpose
    Dual expression vector for bacteria and mammalian cells producing mScarlet-3-H red fluorescent protein
    Depositor
    Dorus Gadella
    Insert
    mScarlet3-H
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242970-plasmid-map-sequence-id-492366

    Enlarge

  46. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Article
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Available Since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    170103-plasmid-map-sequence-id-332603

    Enlarge

  47. pCDF-Frb-v1

    Plasmid
    #201923
    Purpose
    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    Expression
    Bacterial
    Promoter
    T7 (modified), T7 (modified, moderate), T7 (modif…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201923-plasmid-map-sequence-id-395412

    Enlarge

Showing: 951 - 1000 of 31059 results