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32,069 results

Showing: 751 - 780 of 32069 results

  8. pBAD24-sfGFPx2

    Plasmid
    #51559
    Purpose
    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
    Depositor
    Sankar Adhya, Francisco Malagon
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    superfolder GFP
    Tags
    GLESTCRHASLAVLADERRFSA and MARARA
    Expression
    Bacterial
    Mutation
    Contains additonal aa in the N and C terminus (in…
    Available Since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51559-plasmid-map-sequence-id-78831

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  10. MUT

    Plasmid
    #38872
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MUT (Mut Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  15. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  16. CD44I-c001

    Plasmid
    #175448
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175448-plasmid-map-sequence-id-353140

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  21. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  24. psfTn5

    Plasmid
    #79107
    Purpose
    bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations
    Depositor
    Sten Linnarsson
    Insert
    transposase 5
    Tags
    His
    Expression
    Bacterial
    Mutation
    E54K and L372P
    Available Since
    March 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    79107-plasmid-map-sequence-id-418885

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Showing: 751 - 780 of 32069 results