32,156 results
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Plasmid#50388PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertPPAT
Tags6xHis-tagExpressionBacterialPromoterT7Available SinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEST-OR2-OR1-Pr-UTR1-deGFP-T500
Plasmid#40019DepositorInsertdeGFP
ExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available SinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-pBAD
Plasmid#54856PurposeLocalization: Protein Expression Vector , Excitation: 554, Emission: 581DepositorTypeEmpty backboneTags6xHis and tdTomatoExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
ExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLumi-LgBiT
Plasmid#188319PurposeExpression of LgBiT via sigma 70 promoter in E. coliDepositorInsertLgBiT
UseLuciferase and Synthetic BiologyExpressionBacterialPromotersigma 70 promoterAvailable SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET259-pUC57 24xMS2V6
Plasmid#104391PurposeMS2V6 cloningDepositorInsert24xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJWV102-PL-dCas9
Plasmid#85588PurposeIntegrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9spDepositorInsertdCas9sp
UseCRISPRExpressionBacterialPromoterPLspAvailable SinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (A7)
Plasmid#174079PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-mTagBFP2-lenti
Plasmid#115797PurposeExpresses mTagBFP in human cells, contained in a lentiviral vector.DepositorInsertmTagBFP
UseLentiviralExpressionBacterial and MammalianPromoterEF-1a promoterAvailable SinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET GFP LIC cloning vector (2GFP-T)
Plasmid#29716DepositorTypeEmpty backboneTags6xHis, SiriusGFP, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
TagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Rx3(A7)
Plasmid#35525DepositorInsertHis6-Rx3(A7)
TagsHis6ExpressionBacterialMutationChanged G2 to PPromoterT7Available SinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD_FLAG-GFP(Y39TAG)-6His
Plasmid#223510PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
Tags6xHis and FLAGExpressionBacterialMutationY39TAGAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScaf-7560.1
Plasmid#111406PurposepScaf phagemid with insert for generating DNA origami scaffold 7560 bases in lengthDepositorInsertpScaf-7560.1
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin K48C
Plasmid#86588PurposeBacterial expression of Ubiquitin K48C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
TagsHis6-MBPExpressionBacterialMutationTEV site; K48CPromoterTacAvailable SinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDF-SeAvs3
Plasmid#188805PurposeCodon optimized SeAvs3 with C-terminal His tagDepositorInsertSeAvs3
TagsHisExpressionBacterialPromoterT7Available SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin K63C
Plasmid#86589PurposeBacterial expression of Ubiquitin K63C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
TagsHis6-MBPExpressionBacterialMutationTEV site; K63CPromoterTacAvailable SinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
GSK3 beta pGEX
Plasmid#15898DepositorAvailable SinceNov. 1, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDusk
Plasmid#43795PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBad-EBFP2
Plasmid#14891PurposeBacterial expression of EBFP2 blue fluorescent proteinDepositorHas ServiceCloning Grade DNAInsertEBFP2
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pT2/BH
Plasmid#26556DepositorInsertpT2/BH
ExpressionBacterialAvailable SinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
TagsHis-tag followed by thrombin cleavage siteExpressionBacterialPromoterT7Available SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
TagsmClover3ExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD
Plasmid#171235PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)DepositorInsert2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSP90AB1
Plasmid#177659PurposeRecombinant protein synthesis of human HSP90AB1DepositorAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSP3545
Plasmid#46888PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…ExpressionBacterialPromoterPnisAAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
His6-SpyCatcher-mi3
Plasmid#113043PurposeExpresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertHis6-SpyCatcher-mi3
TagsHis-tagExpressionBacterialPromoterT7Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet-I
Plasmid#104007PurposePlasmid encoding mScarlet-IDepositorInsertmScarlet-I
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
V48 ELP
Plasmid#68395PurposeElastin-like polypeptide (VPGxG) with 48 repeats of valine guest residueDepositorInsertV48 ELP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_XVE
Plasmid#136970PurposeGoldengate/Greengate module for generation of beta-estradiole inducible genes.DepositorInsertXVE
ExpressionBacterialMutationBsaI site domestication 834 T > A,Available SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-ATAD2-plx303
Plasmid#188635PurposeATAD2 overexpressionDepositorAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-CRM197-4xDQNAT
Plasmid#128403PurposePeriplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertDiphtheria toxin
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationInactivating G52E mutationPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA15_G-alpha
Plasmid#109782PurposeProtein expression and purification of GNA15_G-alphaDepositorInsertGNA15_G-alpha (GNA15 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
UIMLx2
Plasmid#174888PurposeUIMLx2 ubiquitin probe for K48 ubiquitylated proteinsDepositorInsertMet4 UIML
TagsAvi-6xHis-Smt3ExpressionBacterialAvailable SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS9_10xHis_MBP_TEV_dCas9
Plasmid#244831PurposeBacterial expression of catalytically inactive Cas9 (dCas9) for protein purificationDepositorInsertdCas9 (WT)
UseCRISPRTags10xHis-MBPExpressionBacterialMutationD10A/H840APromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJG103
Plasmid#158533PurposewrmScarlet11 x3 tandem repeatsDepositorInsertwrmScarlet11 x3 tandem repeats
ExpressionBacterialAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PIP4K2AA
Plasmid#42494PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-DogCatcher
Plasmid#171929PurposeExpress Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.DepositorInsertCys-DogCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMW363
Plasmid#238208PurposeExpresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpRDepositorInsertTaTasR
TagsTwinStrepIIExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only