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32,656 results

Showing: 2301 - 2350 of 32656 results

  3. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Promoter
    J23100 promoter
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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  5. T4 Lysozyme WT*

    Plasmid
    #18111
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Expression
    Bacterial
    Mutation
    No cysteines: C54T, C97A
    Available Since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18111-plasmid-map-sequence-id-174143

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  6. MUT

    Plasmid
    #38872
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MUT (Mut Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  12. eSrtA(2A-9) pET29b

    Plasmid
    #75145
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity
    Depositor
    David Liu
    Article
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S102C A104H E105D K138P K152I N160K K162H T164N K…
    Promoter
    T7
    Available Since
    July 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    75145-plasmid-map-sequence-id-466935

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  13. pZP06C

    Plasmid
    #232322
    Purpose
    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.
    Depositor
    Chenggang Wu
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    rpsJ
    Available Since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232322-plasmid-map-sequence-id-462278

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  24. HA-AR-LBD

    Plasmid
    #171239
    Purpose
    Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171239-plasmid-map-sequence-id-336014

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  27. Pet28a_Human_H3.3

    Plasmid
    #42632
    Depositor
    Joe Landry
    Insert
    Human H3.3 (H3-3A Human)
    Tags
    Untagged
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42632-plasmid-map-sequence-id-57747

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  28. pET28a SnoopTag-MBP

    Plasmid
    #72323
    Purpose
    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-MBP
    Tags
    N-terminal His6 tag. MBP is fused at the C-termin…
    Expression
    Bacterial
    Available Since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72323-plasmid-map-sequence-id-137256

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  30. Ydf NanoLuc

    Plasmid
    #225656
    Purpose
    Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NanoLuciferase
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Promoter
    PydfJ115
    Available Since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225656-plasmid-map-sequence-id-455061

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  32. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

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  33. pETM-60-NusA-PfAgo_F

    Plasmid
    #146270
    Purpose
    Bacterial Expression of PfAgo
    Depositor
    Elisa Izaurralde
    Insert
    PfAgo
    Expression
    Bacterial
    Mutation
    one non silent mutation A907G (I303V) and one sil…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146270-plasmid-map-sequence-id-468111

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  35. pET30a-His6-ABP-LOXCATmut

    Plasmid
    #139972
    Purpose
    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139972-plasmid-map-sequence-id-311162

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  37. pMM643

    Plasmid
    #112533
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    luxCDABE
    Expression
    Bacterial
    Mutation
    A17P
    Promoter
    J23107, PL(HrtO), and ProD
    Available Since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112533-plasmid-map-sequence-id-216001

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  41. pBZCas13b

    Plasmid
    #89898
    Purpose
    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89898-plasmid-map-sequence-id-178222

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  44. pNQ071

    Plasmid
    #242082
    Purpose
    Expresses E. coli RNAP (gene inserts: rpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ)
    Depositor
    Olivier Duss
    Article
    Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:
    Insert
    rpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ
    Tags
    His6
    Expression
    Bacterial
    Mutation
    ybbR-peptide tag inserted between E15 and E16 in …
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242082-plasmid-map-sequence-id-486429

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  47. pDOC-K-glmS-GFPrev

    Plasmid
    #158060
    Purpose
    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Insert
    Green Fluorescent Protein optimised for excitation with UV light
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    acpP
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158060-plasmid-map-sequence-id-309440

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  48. 4QL1

    Plasmid
    #61942
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5:APC041-G05:C36415 (WDR5 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 2301 - 2350 of 32656 results