Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,059 results

Showing: 2301 - 2350 of 31059 results

  5. PKMYT1

    Plasmid
    #39061
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PKMYT1 (PKMYT1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

    Enlarge

  17. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120813-plasmid-map-sequence-id-235308

    Enlarge

  20. pSNAP-tag (T7) Vector

    Plasmid
    #101137
    Purpose
    Expression of protein of interest with SNAP-Tag in bacterial cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Tags
    SNAP-tag (SNAP26b)
    Expression
    Bacterial
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101137-plasmid-map-sequence-id-194162

    Enlarge

  24. pOPINE-3C-mCherry

    Plasmid
    #214060
    Purpose
    Expression of fluorescent fusion proteins
    Depositor
    Ray Owens
    Insert
    mCherry
    Tags
    Hexahistidine
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214060-plasmid-map-sequence-id-422614

    Enlarge

  27. pET30a-Tn5

    Plasmid
    #127917
    Purpose
    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.
    Depositor
    Silin Zhong
    Article
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Tn5
    Tags
    Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127917-plasmid-map-sequence-id-254855

    Enlarge

  29. pGEX-2T PTP-1B

    Plasmid
    #8602
    Depositor
    Ben Neel
    Insert
    PTP-1B (PTPN1 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    8602-plasmid-map-sequence-id-151853

    Enlarge

  34. p006kanGFP

    Plasmid
    #58534
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Expression
    Bacterial
    Promoter
    T5 between two lac operators
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58534-plasmid-map-sequence-id-90573

    Enlarge

  35. pHBintT

    Plasmid
    #48134
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48134-plasmid-map-sequence-id-69938

    Enlarge

  36. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

    Enlarge

  37. 6xHis-MBP-huMb2Cas12a

    Plasmid
    #174676
    Purpose
    Expresses 6xHis-MBP tagged Mb2Cas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huMb2Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174676-plasmid-map-sequence-id-346137

    Enlarge

  43. pNB23

    Plasmid
    #103058
    Purpose
    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp
    Depositor
    Dirk Bumann
    Article
    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-
    Insert
    GFP-OVA
    Expression
    Bacterial
    Promoter
    hydrogen peroxide-inducible promoter katGp
    Available Since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103058-plasmid-map-sequence-id-199054

    Enlarge

  44. pPbU6_hdhfr/yfcu_Cas9

    Plasmid
    #216423
    Purpose
    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.
    Depositor
    Ellen Bushell
    Article
    Jonsdottir et al bioRxiv 2024.04.20.590404
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Expression
    Bacterial
    Available Since
    April 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216423-plasmid-map-sequence-id-431289

    Enlarge

  49. pET11b-MIF

    Plasmid
    #149446
    Purpose
    Expression of WT MIF in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149446-plasmid-map-sequence-id-311585

    Enlarge

Showing: 2301 - 2350 of 31059 results