32,656 results
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Plasmid#212156PurposePlasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.DepositorInsertsecondary alcohol dehydrogenase
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pMB1o1_Red
Plasmid#243862PurposeGoldenBraid2.0 expression vector.DepositorInsertmScarlet-I
ExpressionBacterialAvailable SinceFeb. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBR322-sgRNA-pvc18-20
Plasmid#243747PurposePVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNADepositorInsertvegfa sgRNA-pvc18-20
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMGA
Plasmid#139931PurposeM. magneticum/E. coli shuttle vector (pMGT and p15A ori)DepositorInsertsfGFP
ExpressionBacterialPromoterPJ23119Available SinceMarch 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1
Plasmid#78462PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; Q2513R (please see depositor commen…PromoterT7Available SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1-Strep-II
Plasmid#224209PurposeMembrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.DepositorInsertMSP1D1-Strep-II
TagsStrep-II TagExpressionBacterialMutationSubstitution of 7xHis tag found in pMSP1D1 for a …PromoterT7Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE1
Plasmid#73018PurposeExpresses BE1 with N-terminal His6 tag in E. ColiDepositorInsertBE1
TagsHis6 tagExpressionBacterialPromoterT7Available SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Km
Plasmid#45938Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVHed05
Plasmid#82351PurposeContains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetRDepositorInsertsUseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBP-ch-TOG-His6
Plasmid#69108PurposeFor bacterial expression of recombinant human ch-TOG tagged with MBP and His.DepositorInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
TagsHis6 and MBPExpressionBacterialAvailable SinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCF1148
Plasmid#222330PurposeFluorescent reporter plasmid for VeillonellaDepositorInsertbs2 gene with Veillonella parvula SKV38 mdh promoter
UseE. coli-veillonella shuttle plasmidExpressionBacterialPromoterVeillonella parvula SKV38 mdh promoterAvailable SinceOct. 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMP2
Plasmid#79874PurposeBacillus subtilis sgRNA expression vector; integrates into amyEDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable SinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAureus-TnCAM
Plasmid#199807Purposevector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureusDepositorInserttransposon cassette
ExpressionBacterialAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Spike-VLP
Plasmid#173922PurposeExpresses virus-like particles based upon the original SARS-Cov-2DepositorInsertsTagsno tags, native proteinExpressionBacterial and MammalianPromoter22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…Available SinceApril 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTW386c
Plasmid#237321Purposeexpresses O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2DepositorInsertO. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2
ExpressionBacterialAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTOX11
Plasmid#127456PurposeEncodes MqsR toxin. Gent-RDepositorInsertmqsR toxin
ExpressionBacterialPromoterpRhaAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX_sgRNA_entry (CJT32)
Plasmid#181782PurposeExpresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialAvailable SinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE-30-HyPer3
Plasmid#42132PurposeGenetically encoded H2O2 probe for ratiometric and fluorescence lifetime imagingDepositorInsertHYPER 3
Tags6HisExpressionBacterialPromoterT5Available SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
PYCR1
Plasmid#38842PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-gfpmut3
Plasmid#65037PurposeZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteriaDepositorInsertgfpmut3
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterENO1pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_8
Plasmid#59948PurposePT7 GFP reporterDepositorInsertsfGFP
ExpressionBacterialMutationN/APromoterPT7Available SinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas1+2
Plasmid#72676PurposeExpress Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.DepositorInsertCas1, Cas2
ExpressionBacterialAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gi-c001
Plasmid#173078PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_20bpLinker
Plasmid#157787PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_20bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF_LuxR
Plasmid#140039PurposeConstutitive expression of LuxR protein.DepositorInsertpLuxL and LuxR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB070.iodo.5
Plasmid#99397PurposeExpression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanineDepositorInsertPN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
ExpressionBacterialPromoterPN25, Ptet, and PproKAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfpC
Plasmid#111616PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
ExpressionBacterialAvailable SinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pP2-P1(S')
Plasmid#196334PurposePlasmid expressing chimeric P2-P1(S') tail fiberDepositorInsertchimeric tail fiber P2-P1(S') and chaperone protein U'
ExpressionBacterialPromoterP2 late promoterAvailable SinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-FleBt
Plasmid#149302Purpose"Enhances the yields of soluble target proteins in E. coli expression system", "Enable quick and efficient protein purification with high purity and stability"DepositorTypeEmpty backboneTagsFleBt and HRV 3C protease recognition siteExpressionBacterialPromoterT7Available SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS1623
Plasmid#239833PurposeWT OrufIscB E. coli expression for recombinant purificationDepositorInsertOrufIscB
Tags14xHis-TwinStrep-bdSUMOExpressionBacterialPromoterT7Available SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJUMP19-Ptet_P
Plasmid#127005PurposeBasic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetRDepositorInsertPart Ptet_P
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-tetA-Ptet-Rtet
Plasmid#189855PurposeCre-inducible activation of tetA for tetracycline resistance.DepositorInsertPtet-loxP-TT-loxP-Rtet-tetA
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterPtetAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmGFP
Plasmid#186542PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmidDepositorInsertLSSmGFP
Tags6xHisExpressionBacterialMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoteraraBAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO PPM1H
Plasmid#207333PurposeBacterial expression of His SUMO PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP1189
Plasmid#119257Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_rfp
Plasmid#194155PurposeRed Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
ExpressionBacterialPromoterPoxyR, PoxySAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-mCherry
Plasmid#213862PurposeBinary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused mCherry-UnaG
ExpressionBacterial and PlantAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-GST-Tev-Af1521-myc
Plasmid#196239PurposeN-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminusDepositorInsertN-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
TagsGST tag and myc tagExpressionBacterialAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-GreenPy1Highest
Plasmid#239049PurposeBacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1HighestDepositorInsertGreenPy1Highest
ExpressionBacterialAvailable SinceJune 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA
Plasmid#190790Purpose[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.DepositorInsertsCas9
mazF-LVA
UseCRISPR and Synthetic BiologyTagsLVAExpressionBacterialMutationsilent mutations of BpiI-sites in original Cas9 g…PromoterPnrsB and Ptrc-[E*](104)Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pQE81L-KOFP7
Plasmid#160330PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.DepositorInsertKOFP7
Tags6xHis tagExpressionBacterialPromoterT5Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-HaloTag7-SnoopLigase
Plasmid#105627PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7DepositorInsertHaloTag7-SnoopLigase
ExpressionBacterialPromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KEAP1_KELCH
Plasmid#178476PurposeBacterial expression of human domain with His-tag and MBP tagDepositorAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-NUSA-TEV-GG
Plasmid#215798PurposeCloning of target gene with N terminal His6 NUSA tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits