Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,069 results

Showing: 3521 - 3560 of 32069 results

  2. pSW002-Pc-mRuby2

    Plasmid
    #111250
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    mRuby2
    Expression
    Bacterial
    Mutation
    mRuby2 is codon optimized for expression in P. fl…
    Promoter
    Pc
    Available Since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111250-plasmid-map-sequence-id-215255

    Enlarge

  11. pGP-Tn7-Pcym

    Plasmid
    #225280
    Purpose
    Mini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.
    Depositor
    Mark Schembri
    Article
    Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    Pcym cumate (p-isopropylbenzoate)-inducible promo…
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    225280-plasmid-map-sequence-id-457260

    Enlarge

  12. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

    Enlarge

  14. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134342-plasmid-map-sequence-id-306400

    Enlarge

  30. Mut2-RS

    Plasmid
    #182882
    Purpose
    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
    Depositor
    Miriam Amiram
    Article
    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384
    Insert
    Mut2-RS
    Expression
    Bacterial
    Available Since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    182882-plasmid-map-sequence-id-366061

    Enlarge

  38. p11_AaC60αβ/C20

    Plasmid
    #229506
    Purpose
    Expresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Chaperonin 60α
    Chaperonin 60β
    Chaperonin 20
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229506-plasmid-map-sequence-id-456756

    Enlarge

  40. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

    Enlarge

Showing: 3521 - 3560 of 32069 results