29,729 results
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Plasmid#228113PurposepET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoterDepositorInsertKva1 retron, ncRNA with 90 bp rpoB donor
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP E17TAG
Plasmid#68295PurposeE17TAG GFP reporterDepositorInsertE17TAG GFP
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRT498-3xOtUBD
Plasmid#190088PurposeExpresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsert3xOtUBD
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pROP-PP-FBFP
Plasmid#122137PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)DepositorInsertFBFP
UseTagsExpressionBacterialMutationPromoterJ23101Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNM58
Plasmid#128409PurposeSystem with AIP and xylose inducible agrCA sensorDepositorInsertsargCA
gfpmut2
UseTagsExpressionBacterialMutationPromoterP3 and PxylRAvailable sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis-hMTMR1
Plasmid#100723PurposeExpression of human MTMR1 PH-PTP domainDepositorInsertMTMR1 (MTMR1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 95-607PromoterT7Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREDusk-AmpR-MCS
Plasmid#188974PurposeFor red light-repressed bacterial expression of gene of interest (AmpR)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Reb_206 aka PAS629
Plasmid#71234PurposeExpresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherryDepositorInsertReb locus
UseTagsmNeonGreen to second copy RebB, soluble mCherry a…ExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA6c-RFP
Plasmid#35290DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLlacO-1Available sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
SARS_pNIC-CTB10H
Plasmid#153056PurposeExpression construct for producing Avi tagged Seryl-tRNA synthetaseDepositorInsertSARS (SARS1 Human)
UseTagsAviExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
UseTagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PAmCherry1
Plasmid#31931DepositorInsertPAmCherry1
UseTagsHisExpressionBacterialMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp6-His
Plasmid#11823DepositorInsertCaspase 6 (CASP6 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-R-GECO1
Plasmid#32465DepositorInsertR-GECO1.0
UseLab constructedTags6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…ExpressionBacterialMutationSubstitutions relative to the mApple-derived anal…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
Stat2 pBluescript SK (-)
Plasmid#8705DepositorInsertStat2 (STAT2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDDYFP-A
Plasmid#40288DepositorInsertddYFP-A
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMART-SOS-GFPuv
Plasmid#102283PurposeFluorescent sensor of E. coli SOS responseDepositorInsertcda-GFPuv
UseTagsExpressionBacterialMutationPromotercda SOS promoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_OriM
Plasmid#110097PurposeAnalogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn1 pcDNA3
Plasmid#36263DepositorInsertDynamin 1 (Dnm1 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-mGSDMD
Plasmid#111560PurposeExpress mouse GSDMD in E.coliDepositorInsertGasdermin D (Gsdmd Mouse)
UseTagsHis, SUMOExpressionBacterialMutationPromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Gamma crystallin TEV LIC cloning vector (1X)
Plasmid#29661DepositorTypeEmpty backboneUseTags6xHis, Gamma-crystallin, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_05
Plasmid#194666PurposeProtein production of the split HaloTag based calcium recorder Caprola_05 in bacteriaDepositorInsertCaprola_05
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWUKI 1+2
Plasmid#79879PurposeEncodes Cas1+2 and a CRISPR arrayDepositorInsertsCas1
Cas2
CRISPR Array
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-XBP1u-FLuc
Plasmid#31239DepositorInsertFLAG-XBP1u-FLuc (XBP1 Human)
UseTagsFlag and FlucExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SAR1-no stop
Plasmid#67345PurposehSar1 without stop codon in Gateway Entry vectorDepositorInsertSAR1 (SAR1A Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET His6 StrepII TEV LIC cloning vector (2HR-T)
Plasmid#29718DepositorTypeEmpty backboneUseTags6xHis, Strep Ii, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
Pet28a-His-tag-BS2
Plasmid#220347PurposeE. coli expression vector to purify BS2DepositorInsertBS2
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET_RGG-RGG-RGG
Plasmid#124938PurposeExpression of RGG-RGG-RGGDepositorInsertRGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_YBX3-Halo
Plasmid#175308PurposeProduction of HaloTag fusion proteinDepositorInsertYBX3 (YBX3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM643
Plasmid#112533PurposepZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
UseTagsExpressionBacterialMutationA17PPromoterJ23107, PL(HrtO), and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-143
Plasmid#118559PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCM18
Plasmid#78565Purpose60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmidDepositorTypeEmpty backboneUseTagsluxCDABEExpressionBacterialMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE165
Plasmid#118513Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET14b – lambda exonuclease
Plasmid#104531Purposebacterial expression of lambda exonucleaseDepositorInsertλ exonuclease
UseTagsHis6 followed by a thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)/MBP-His
Plasmid#38006DepositorInsertMBP (Maltose binding protein)
UseTags6xHISExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalpha
Plasmid#160229Purposeplaces LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal platesDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
TS246_pNKI-2-13_HALO-DPN7
Plasmid#200720Purposeproducing Methyl Tracer HALO protein in bacteriaDepositorInsertHALO-DPN7
UseTags6xHisExpressionBacterialMutationcodon optimizedPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLZL2386
Plasmid#48682PurposepET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)DepositorInsertPFV Integrase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaE (pESC124)
Plasmid#50390PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertDPCK
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmGFP
Plasmid#186542PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmidDepositorInsertLSSmGFP
UseTags6xHisExpressionBacterialMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
As0
Plasmid#123158PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS67, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2-gfp
Plasmid#109290PurposeExpresses high levels of gfp in mycobacteriaDepositorInsertGFP
UseTagsExpressionBacterialMutationWTPromoterKanamycinAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512_Mut235
Plasmid#179278PurposeExpresses Br512_Mut235 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialMutationPromoterT7Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTlpA-Wasabi-NF
Plasmid#86100PurposeNonfluorescent variant of mWasabi reporter gene gated by wild type TlpADepositorInsertsTlpA
mWasabi
UseTags6xHisExpressionBacterialMutationD341A and S71T, G73APromoterTlpA and TlpA, LacIAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recAmu-sacB
Plasmid#158717PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.DepositorInsertrecA
UseTagsExpressionBacterialMutationR61CPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLZ12Km2-P23R:TA:fflucRT
Plasmid#88901PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + Red-shifted firefly luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC101-ΔPS-PM,CS-RBSBBa_B0034-geneVI
Plasmid#134356PurposeAccessory plasmidDepositorInsertGene VI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pMXs-Runx1
Plasmid#131609Purposeexpression of mouse Runx1 in mammalian cellsDepositorInsertRunx1 (Runx1 Mouse)
UseRetroviralTagsExpressionBacterialMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CTHF
Plasmid#26105PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsTEV - His6 - FlagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only