Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,059 results

Showing: 3681 - 3720 of 31059 results

  3. pET28a Gx-mNG-NL

    Plasmid
    #169106
    Purpose
    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - mNeonGreen - NanoLuc
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169106-plasmid-map-sequence-id-335361

    Enlarge

  9. pDule-Acd82

    Plasmid
    #174099
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174099-plasmid-map-sequence-id-345716

    Enlarge

  15. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204956-plasmid-map-sequence-id-415598

    Enlarge

  19. HA-AR-LBD

    Plasmid
    #171239
    Purpose
    Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171239-plasmid-map-sequence-id-336014

    Enlarge

  26. pET21a IHF

    Plasmid
    #26641
    Depositor
    Phoebe Rice
    Insert
    IHF
    Expression
    Bacterial
    Available Since
    Oct. 22, 2010
    Availability
    Academic Institutions and Nonprofits only

  30. pTarget

    Plasmid
    #214052
    Purpose
    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Target sequence
    Use
    Target rna plasmid
    Expression
    Bacterial
    Promoter
    trc promoter
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214052-plasmid-map-sequence-id-428822

    Enlarge

  31. pNon-target

    Plasmid
    #214053
    Purpose
    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Non-target sequence
    Use
    Non-target rna plasmid
    Expression
    Bacterial
    Promoter
    trc promoter
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214053-plasmid-map-sequence-id-428839

    Enlarge

  32. pGLO-Magenta-AZA

    Plasmid
    #220784
    Purpose
    Expresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Angela Leu
    Insert
    magenta chromoprotein
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220784-plasmid-map-sequence-id-437914

    Enlarge

Showing: 3681 - 3720 of 31059 results