Skip to main content
Addgene
Showing: 3801 - 3840 of 29646 results

  9. pGEX6p1-GST-Rnf31

    Plasmid
    #63134
    Purpose
    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells
    Depositor
    Titia Sixma
    Article
    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:
    Insert
    Rnf31 (RNF31 Human, Synthetic)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Synthetic based on human protein sequence
    Promoter
    tac
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pTO_CHMP3_LAP

    Plasmid
    #101849
    Purpose
    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101849-plasmid-map-sequence-id-200249

    Enlarge

  17. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Use
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

    Enlarge

  18. pET-Sac-Abeta(MC1-40)

    Plasmid
    #127152
    Purpose
    Expresses N-terminal cysteine Abeta(1-40)
    Depositor
    James Nowick
    Insert
    Abeta(MC1-40) (APP Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127152-plasmid-map-sequence-id-247836

    Enlarge

  19. pET28a MDHC_YEAST

    Plasmid
    #204232
    Purpose
    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHC_YEAST (MDH2 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204232-plasmid-map-sequence-id-413337

    Enlarge

  24. p189

    Plasmid
    #65207
    Purpose
    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid
    #172505
    Purpose
    Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172505-plasmid-map-sequence-id-344925

    Enlarge

  28. pST44-AP-1 core

    Plasmid
    #184809
    Purpose
    Bacterial expression of clathrin adaptor AP-1 core complex
    Depositor
    James Hurley
    Article
    Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10
    Insert
    Hs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
    Use
    Tags
    GST tag and His6
    Expression
    Bacterial
    Mutation
    AP1S3: S148C; AP1B1: K359R, E476K
    Promoter
    T7
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    184809-plasmid-map-sequence-id-365328

    Enlarge

  29. pPGC-K

    Plasmid
    #174581
    Purpose
    pOPIN-GG expression vector compatible with C-terminal tagging, Kanamycin resistant
    Depositor
    Mark Banfield
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174581-plasmid-map-sequence-id-344698

    Enlarge

  32. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. pTmpKmH207in

    Plasmid
    #176230
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176230-plasmid-map-sequence-id-347005

    Enlarge

  38. pTmpKmH207out

    Plasmid
    #176231
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176231-plasmid-map-sequence-id-347006

    Enlarge

  39. pTmpKmA1O4in

    Plasmid
    #176232
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176232-plasmid-map-sequence-id-347007

    Enlarge

  40. pTmpKmA1O4out

    Plasmid
    #176233
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176233-plasmid-map-sequence-id-347008

    Enlarge

Showing: 3801 - 3840 of 29646 results