Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Mammalian Expression

76,325 results

Showing: 7151 - 7200 of 76325 results

  1. PA-mCherry-Alpha-5-Integrin-12

    Plasmid
    #62790
    Purpose
    Localization: Focal Adhesions, Excitation:400 / 504, Emission:515 / 517
    Depositor
    Michael Davidson
    Insert
    Alpha5Integrin (ITGAV Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. LRP4-Flag/pCDNA3.1

    Plasmid
    #197291
    Purpose
    Plasmid expressing an antigen targeting LRP4 (extracellular)
    Depositor
    Steve Burden
    Insert
    LRP4 (Lrp4 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197291-plasmid-map-sequence-id-388074

    Enlarge

  8. SSTR2-Tango

    Plasmid
    #66503
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    SSTR2 (SSTR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66503-plasmid-map-sequence-id-345433

    Enlarge

  9. Clover2-H2B-6

    Plasmid
    #56536
    Purpose
    Localization: Nucleus/Histones, Excitation: 505, Emission: 515
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    Clover2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56536-plasmid-map-sequence-id-269078

    Enlarge

  22. OPRM1-Tango

    Plasmid
    #66464
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OPRM1 (OPRM1 Human)
    Use
    Tags
    FLAG and tTA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66464-plasmid-map-sequence-id-157903

    Enlarge

  25. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Use
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102643-plasmid-map-sequence-id-196911

    Enlarge

  30. pcDNA3-PRB

    Plasmid
    #89130
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418
    Depositor
    Elizabeth Wilson
    Article
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Insert
    human progesterone receptor-B (PGR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89130-plasmid-map-sequence-id-176077

    Enlarge

  31. GABA BR2-flag/pRK5

    Plasmid
    #197323
    Purpose
    Plasmid expressing an antigen targeting the GABA(B)R2 metabotropic GABA receptor
    Depositor
    Stephen Moss
    Insert
    GABA B R2 (Gabbr2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    197323-plasmid-map-sequence-id-392892

    Enlarge

  40. Plenti-ARE-HSVTK-luc

    Plasmid
    #161789
    Purpose
    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers
    Depositor
    Hans-Guido Wendel
    Article
    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi
    Inserts
    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and Murine Gclc ARE
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161789-plasmid-map-sequence-id-324302

    Enlarge

  41. pcDNA3.1-OGT_opt

    Plasmid
    #190858
    Purpose
    For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells.
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190858-plasmid-map-sequence-id-379243

    Enlarge

  43. pCAGG-NFIX1

    Plasmid
    #112702
    Purpose
    Expression of NFIX1 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I X1 (Nfix Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112702-plasmid-map-sequence-id-212396

    Enlarge

  45. pcDNA3.1.Gα12-LgB115

    Plasmid
    #134363
    Purpose
    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα12-LgB115 (GNA12 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134363-plasmid-map-sequence-id-306384

    Enlarge

Showing: 7151 - 7200 of 76325 results